Microbiology 020 - General Microbiology » Fall 2020 » Lab Quiz 10

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Question #1
A change in a single nucleotide can result in…
A.   all of these are correct
B.   a missense mutation
C.   a silent mutation
D.   a nonsense mutation
Question #2
DNA exhibits complementary base pairing. Adenine only base pairs with ________ and cytosine only base pairs with ________.
A.   thymine; guanine
B.   guanine; uracil
C.   guanine; thymine
D.   thymine; uracil
Question #3
DNA and RNA are structurally similar, but they have different functions.
A.   FALSE
B.   TRUE
Question #4
If the DNA strand has the sequence AATTCGAC, what would the complementary RNA strand be?
A.   UUAAGCUG
B.   AAUUCGCAC
C.   TTUUGCTC
D.   Two of these are correct
Question #5
__________ is the process used to release and isolate DNA from cells. It will result in a solution in which particles are evenly dispersed.
A.   homogenization
B.   oxidation
C.   hydrogenation
D.   emulsification
Question #6
To avoid damaging the DNA isolate, a glass rod is used and spun in one direction.
A.   FALSE
B.   TRUE
Question #7
What is the purpose of adding detergent during DNA isolation?
A.   To degrade DNA
B.   To disrupt cell membranes and release DNA into solution
C.   To stabilize DNA
D.   To condense DNA
Question #8
What happens if you set a low voltage during electrophoresis?
A.   It can can heat up the buffer and cause the gel to melt
B.   The migration of the DNA bands will be slow
C.   The DNA will migrate in the wrong direction.
D.   The DNA will not be activated and cannot be visualized in UV light
Question #9
The electrophoresis buffer must be added to the chamber ______ loading the wells with samples.
A.   after
B.   before
Question #10
What is the purpose of the plexiglass screen of the UV-table?
A.   None of these are correct
B.   The plexiglass screen protects the eyes against UV-radiation
C.   The plexiglass protects against the heat caused by UV-radiation of the gel
D.   The plexiglass is a cover to keep the UV-table clean

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