Microbiology 020 - General Microbiology » Fall 2020 » Lab Quiz 10

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Question #1
A change in a single nucleotide can result in…
A.   a nonsense mutation
B.   all of these are correct
C.   a silent mutation
D.   a missense mutation
Question #2
DNA exhibits complementary base pairing. Adenine only base pairs with ________ and cytosine only base pairs with ________.
A.   thymine; uracil
B.   thymine; guanine
C.   guanine; uracil
D.   guanine; thymine
Question #3
DNA and RNA are structurally similar, but they have different functions.
A.   FALSE
B.   TRUE
Question #4
If the DNA strand has the sequence AATTCGAC, what would the complementary RNA strand be?
A.   Two of these are correct
B.   UUAAGCUG
C.   TTUUGCTC
D.   AAUUCGCAC
Question #5
__________ is the process used to release and isolate DNA from cells. It will result in a solution in which particles are evenly dispersed.
A.   homogenization
B.   oxidation
C.   emulsification
D.   hydrogenation
Question #6
To avoid damaging the DNA isolate, a glass rod is used and spun in one direction.
A.   FALSE
B.   TRUE
Question #7
What is the purpose of adding detergent during DNA isolation?
A.   To disrupt cell membranes and release DNA into solution
B.   To degrade DNA
C.   To condense DNA
D.   To stabilize DNA
Question #8
What happens if you set a low voltage during electrophoresis?
A.   The migration of the DNA bands will be slow
B.   The DNA will not be activated and cannot be visualized in UV light
C.   The DNA will migrate in the wrong direction.
D.   It can can heat up the buffer and cause the gel to melt
Question #9
The electrophoresis buffer must be added to the chamber ______ loading the wells with samples.
A.   after
B.   before
Question #10
What is the purpose of the plexiglass screen of the UV-table?
A.   None of these are correct
B.   The plexiglass protects against the heat caused by UV-radiation of the gel
C.   The plexiglass is a cover to keep the UV-table clean
D.   The plexiglass screen protects the eyes against UV-radiation

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