Microbiology 020 - General Microbiology » Fall 2020 » Simple and Negative Stain

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Question #1
Smears are fixed by heat.
A.   FALSE
B.   TRUE
Question #2
Smear preparation is the same for liquid and solid cultures.
A.   TRUE
B.   FALSE
Question #3
________ is used for negative staining, _______ while is used for simple staining.
A.   acidic dye, basic dye
B.   basic dye, acidic dye,
C.   basic dye, neutral dye,
D.   neutral dye, acidic dye,
Question #4
Which of the following is (are) basic dyes ? Choose all that apply.,,
A.   crystal violet
B.   methylene blue
C.   nigrosine
D.   safranin
Question #5
What is the purpose of fixing the smear?
A.   to prevent it from discoloration
B.   to prevent it from washing off
C.   to prevent it from breaking
Question #6
What is the shape and arrangement of Staphylococcus aureus?
A.   triangular and lined together
B.   triangular and clumped together
C.   spherical and lined together
D.   spherical and clumped together
Question #7
Quality control staff in a sterilization unit of a hospital used a simple stain to determine whether bacteria present in sterilized materials. A simple stain of sterile saline used for respiratory therapy revealed the presence of bacteria. Is the saline contaminated?
A.   NO
B.   YES

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