Bio 2010 - Microbiology » Spring 2021 » Streak for Isolation Technique

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Question #1
Where should you label your Petri plate with information about the culture?
A.   on the inside of the lid.
B.   on the bottom of the plate.
C.   on the side of the bottom (agar-containing) portion of the plate.
D.   on the top of the lid.
Question #2
After each streak on the plate with the inoculating loop, what is done to the loop?
A.   discarded
B.   flamed until loop glows
C.   re-introduced to stock culture
D.   wiped with disinfectant
Question #3
Imagine that you forgot to flame the loop before streaking the inoculum from the first sector/quadrant into the second sector/quadrant. What is the most likely consequence of this error?
A.   contamination of the Petri plate culture.
B.   contamination of the broth culture.
C.   too little bacterial growth outside the first sector/quadrant.
D.   too much bacterial growth outside the first sector/quadrant.
Question #4
What does the streak technique enable us to achieve?
A.   direct spread and dilution of bacteria from a single colony
B.   visualization of virus plaques
C.   isolation of bacterial colonies
D.   development of antibacterial microbes
Question #5
Which of the following observations would suggest that a plate was inoculated with a pure culture?
A.   bacterial growth is apparent along the streaks connecting each quadrant.
B.   isolated colonies have uniform color and same size.
C.   bacterial growth is apparent in all four quadrants.
D.   isolated colonies are all white in color, but some colonies are noticeably larger than others.

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