Bio 2010 - Microbiology » Spring 2021 » Streak for Isolation Technique

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Question #1
Where should you label your Petri plate with information about the culture?
A.   on the bottom of the plate.
B.   on the inside of the lid.
C.   on the top of the lid.
D.   on the side of the bottom (agar-containing) portion of the plate.
Question #2
After each streak on the plate with the inoculating loop, what is done to the loop?
A.   re-introduced to stock culture
B.   discarded
C.   wiped with disinfectant
D.   flamed until loop glows
Question #3
Imagine that you forgot to flame the loop before streaking the inoculum from the first sector/quadrant into the second sector/quadrant. What is the most likely consequence of this error?
A.   too much bacterial growth outside the first sector/quadrant.
B.   too little bacterial growth outside the first sector/quadrant.
C.   contamination of the broth culture.
D.   contamination of the Petri plate culture.
Question #4
What does the streak technique enable us to achieve?
A.   visualization of virus plaques
B.   development of antibacterial microbes
C.   direct spread and dilution of bacteria from a single colony
D.   isolation of bacterial colonies
Question #5
Which of the following observations would suggest that a plate was inoculated with a pure culture?
A.   isolated colonies are all white in color, but some colonies are noticeably larger than others.
B.   bacterial growth is apparent in all four quadrants.
C.   bacterial growth is apparent along the streaks connecting each quadrant.
D.   isolated colonies have uniform color and same size.

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