Bio 2010 - Microbiology » Spring 2021 » Streak for Isolation Technique

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Question #1
Where should you label your Petri plate with information about the culture?
A.   on the side of the bottom (agar-containing) portion of the plate.
B.   on the bottom of the plate.
C.   on the top of the lid.
D.   on the inside of the lid.
Question #2
After each streak on the plate with the inoculating loop, what is done to the loop?
A.   wiped with disinfectant
B.   flamed until loop glows
C.   re-introduced to stock culture
D.   discarded
Question #3
Imagine that you forgot to flame the loop before streaking the inoculum from the first sector/quadrant into the second sector/quadrant. What is the most likely consequence of this error?
A.   contamination of the broth culture.
B.   too much bacterial growth outside the first sector/quadrant.
C.   contamination of the Petri plate culture.
D.   too little bacterial growth outside the first sector/quadrant.
Question #4
What does the streak technique enable us to achieve?
A.   direct spread and dilution of bacteria from a single colony
B.   isolation of bacterial colonies
C.   development of antibacterial microbes
D.   visualization of virus plaques
Question #5
Which of the following observations would suggest that a plate was inoculated with a pure culture?
A.   isolated colonies are all white in color, but some colonies are noticeably larger than others.
B.   bacterial growth is apparent along the streaks connecting each quadrant.
C.   bacterial growth is apparent in all four quadrants.
D.   isolated colonies have uniform color and same size.

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