Bio 2010 - Microbiology » Spring 2021 » Streak for Isolation Technique

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Question #1
Where should you label your Petri plate with information about the culture?
A.   on the top of the lid.
B.   on the inside of the lid.
C.   on the bottom of the plate.
D.   on the side of the bottom (agar-containing) portion of the plate.
Question #2
After each streak on the plate with the inoculating loop, what is done to the loop?
A.   re-introduced to stock culture
B.   wiped with disinfectant
C.   flamed until loop glows
D.   discarded
Question #3
Imagine that you forgot to flame the loop before streaking the inoculum from the first sector/quadrant into the second sector/quadrant. What is the most likely consequence of this error?
A.   contamination of the Petri plate culture.
B.   too much bacterial growth outside the first sector/quadrant.
C.   too little bacterial growth outside the first sector/quadrant.
D.   contamination of the broth culture.
Question #4
What does the streak technique enable us to achieve?
A.   development of antibacterial microbes
B.   direct spread and dilution of bacteria from a single colony
C.   visualization of virus plaques
D.   isolation of bacterial colonies
Question #5
Which of the following observations would suggest that a plate was inoculated with a pure culture?
A.   bacterial growth is apparent in all four quadrants.
B.   isolated colonies have uniform color and same size.
C.   isolated colonies are all white in color, but some colonies are noticeably larger than others.
D.   bacterial growth is apparent along the streaks connecting each quadrant.

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