Bio 2010 - Microbiology » Spring 2021 » Gram Stain Technique

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Question #1
Which of the following is the Gram's destain or bleaching reagent?
A.   Loeffler's methylene blue
B.   Crystal violet
C.   Carbolfuchsin
D.   Safranin
E.   Acetone- Alcohol
Question #2
Why is it important that you don’t put too much water to prepare the smear?
A.   Water may carry contaminants
B.   It will cause the cells to swim away
C.   It will take too long to dry the smear
D.   Water can dilute the stain
Question #3
You and your group partner were engaged in a conversation while performing your Gram stain. You realized you might have applied acetone alcohol on your smear for 1 minute. What would you do?
A.   Start all over again because your Gram-positive cells may have lost its primary stain
B.   Add crystal violet before adding safranin, which will fix the problem
C.   Mix safranin with crystal violet
D.   You did the right thing, continue with the next step
Question #4
Which of the following are the correct order of chemicals used in Gram staining?
A.   Iodine, Acetone-alcohol, crystal violet, safranin
B.   Crystal violet, iodine, acetone-alcohol, safranin
C.   Safranin, crystal violet, iodine, acetone-alcohol
D.   Crystal violet, iodine, safranin, acetone-alcohol

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