Bio 2010 - Microbiology » Spring 2021 » Gram Stain Technique

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Question #1
Which of the following is the Gram's destain or bleaching reagent?
A.   Acetone- Alcohol
B.   Crystal violet
C.   Carbolfuchsin
D.   Loeffler's methylene blue
E.   Safranin
Question #2
Why is it important that you don’t put too much water to prepare the smear?
A.   It will cause the cells to swim away
B.   Water may carry contaminants
C.   It will take too long to dry the smear
D.   Water can dilute the stain
Question #3
You and your group partner were engaged in a conversation while performing your Gram stain. You realized you might have applied acetone alcohol on your smear for 1 minute. What would you do?
A.   You did the right thing, continue with the next step
B.   Start all over again because your Gram-positive cells may have lost its primary stain
C.   Mix safranin with crystal violet
D.   Add crystal violet before adding safranin, which will fix the problem
Question #4
Which of the following are the correct order of chemicals used in Gram staining?
A.   Crystal violet, iodine, acetone-alcohol, safranin
B.   Iodine, Acetone-alcohol, crystal violet, safranin
C.   Crystal violet, iodine, safranin, acetone-alcohol
D.   Safranin, crystal violet, iodine, acetone-alcohol

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