Bio 2010 - Microbiology » Spring 2021 » Chapter 4 Quiz
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Question #1
A microscope that exposes specimens to ultraviolet light and forms an image with the light emitted at a different wavelength is called a __________ microscope.
A.
scanning electron
B.
phase contrast
C.
fluorescent
D.
darkfield
Question #2
Phase Contrast microscopy is different than bright field microscopy because:
A.
Continuously changes the phase of the incident light from the condenser to improve contrast in the specimen.
B.
Uses filters in the objective to give contrast to parts of the cell with different thicknesses
C.
Uses special lenses to change the color of light passing through them.
D.
Uses special lenses to distinguish between solid and liquid phases of the cell
Question #3
Which of the following has the BEST resolution?
A.
dark field microscopy
B.
bright field microscopy
C.
Phase contrast
D.
differential interference contrast (DIC)
E.
none of the above
Question #4
Light of ____ wavelength typically will result in ____ resolving power.
A.
any, poor
B.
shorter, better
C.
longer, better
D.
shorter, worse
Question #5
Which of the following microscope types has the best contrast?
A.
differential interference contrast (DIC)
B.
phase contrast
C.
dark field miscroscopy
D.
bright field miscroscopy
Question #6
A compound light microscope allows clear visualization of objects no smaller than a:
A.
four leaf clover
B.
typical virus
C.
human cell
D.
DNA molecule
E.
small bacterium
Question #7
The kind of electron microscope which is used to study internal structure of cells is:
A.
transmission electron microscope
B.
scanning electron microscope
C.
light microscope
D.
compound microscope
Question #8
The transmission electron microscope has the greatest resolving power because it uses an electron beam to view the sample instead of a light beam. The electron beam is used because:
A.
Electrons have longer wavelengths than light waves
B.
Light waves are less visible.
C.
Electrons have shorter wavelengths than light waves
D.
Electrons are less invasive
E.
Electrons do not penetrate the sample.
Question #9
You want to determine the location of a specific protein in a cell, using a color stain. Which of the following is the BEST type of microscopy to use?
A.
bright field microscopy
B.
fluorescent microscopy
C.
dark field microscopy
D.
electron microscopy
E.
phase contrast microsocpy
Question #10
Which of the following is used to visualize live cells?
A.
Phase Contrast
B.
TEM
C.
Phase Contrast, SEM and TEM
D.
SEM
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