Bio 2010 - Microbiology » Spring 2021 » Chapter 4 Quiz

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Question #1
A microscope that exposes specimens to ultraviolet light and forms an image with the light emitted at a different wavelength is called a __________ microscope.
A.   scanning electron
B.   darkfield
C.   fluorescent
D.   phase contrast
Question #2
Phase Contrast microscopy is different than bright field microscopy because:
A.   Uses special lenses to distinguish between solid and liquid phases of the cell
B.   Uses special lenses to change the color of light passing through them.
C.   Continuously changes the phase of the incident light from the condenser to improve contrast in the specimen.
D.   Uses filters in the objective to give contrast to parts of the cell with different thicknesses
Question #3
Which of the following has the BEST resolution?
A.   Phase contrast
B.   bright field microscopy
C.   dark field microscopy
D.   differential interference contrast (DIC)
E.   none of the above
Question #4
Light of ____ wavelength typically will result in ____ resolving power.
A.   shorter, better
B.   longer, better
C.   any, poor
D.   shorter, worse
Question #5
Which of the following microscope types has the best contrast?
A.   bright field miscroscopy
B.   differential interference contrast (DIC)
C.   phase contrast
D.   dark field miscroscopy
Question #6
A compound light microscope allows clear visualization of objects no smaller than a:
A.   four leaf clover
B.   small bacterium
C.   human cell
D.   DNA molecule
E.   typical virus
Question #7
The kind of electron microscope which is used to study internal structure of cells is:
A.   light microscope
B.   scanning electron microscope
C.   transmission electron microscope
D.   compound microscope
Question #8
The transmission electron microscope has the greatest resolving power because it uses an electron beam to view the sample instead of a light beam. The electron beam is used because:
A.   Light waves are less visible.
B.   Electrons have longer wavelengths than light waves
C.   Electrons are less invasive
D.   Electrons do not penetrate the sample.
E.   Electrons have shorter wavelengths than light waves
Question #9
You want to determine the location of a specific protein in a cell, using a color stain. Which of the following is the BEST type of microscopy to use?
A.   dark field microscopy
B.   fluorescent microscopy
C.   phase contrast microsocpy
D.   bright field microscopy
E.   electron microscopy
Question #10
Which of the following is used to visualize live cells?
A.   Phase Contrast
B.   SEM
C.   Phase Contrast, SEM and TEM
D.   TEM

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