Bio 2010 - Microbiology » Summer 2021 » Aseptic Transfer Technique

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Question #1
How should you sterilize materials that will make contact with your culture media?
A.   more than one of the above is correct
B.   wash them in the sink
C.   by using the Bunsen burner and flaming them
D.   you don't need to sterilize materials that will make contact with your experiment
E.   wiping it down with a tissue
F.   none of the above is correct
Question #2
What is the name of the instrument that we MUST use during the aseptic microorganism transfer during our lab activities?
A.   flame loop
B.   pipettor
C.   finger
D.   flame wand
Question #3
Which of the following would be the BEST time to label test tubes and Petri plates?
A.   before you use them
B.   after using them
C.   more than one of the above is correct
D.   while you are using them
E.   none of the above is correct
Question #4
You and your partner were talking during your aseptic transfer activity. You cannot remember if you flamed your flame loop before you opened your first test tube. What should you do in order to avoid contamination?
A.   get a different flame loop
B.   flame it again just to be sure.
C.   nothing, bacteria cannot grow on the metal loop
D.   nothing, the last person flamed it
Question #5
Once inoculated with bacteria, how should agar Petri plates be incubated?
A.   right side up
B.   sideways
C.   upside down
D.   more than one of the above is correct
E.   none of the above is correct

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