Bio 2010 - Microbiology » Summer 2021 » Aseptic Transfer Technique

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Question #1
How should you sterilize materials that will make contact with your culture media?
A.   wash them in the sink
B.   more than one of the above is correct
C.   you don't need to sterilize materials that will make contact with your experiment
D.   by using the Bunsen burner and flaming them
E.   wiping it down with a tissue
F.   none of the above is correct
Question #2
What is the name of the instrument that we MUST use during the aseptic microorganism transfer during our lab activities?
A.   pipettor
B.   finger
C.   flame loop
D.   flame wand
Question #3
Which of the following would be the BEST time to label test tubes and Petri plates?
A.   after using them
B.   more than one of the above is correct
C.   while you are using them
D.   before you use them
E.   none of the above is correct
Question #4
You and your partner were talking during your aseptic transfer activity. You cannot remember if you flamed your flame loop before you opened your first test tube. What should you do in order to avoid contamination?
A.   nothing, bacteria cannot grow on the metal loop
B.   get a different flame loop
C.   nothing, the last person flamed it
D.   flame it again just to be sure.
Question #5
Once inoculated with bacteria, how should agar Petri plates be incubated?
A.   more than one of the above is correct
B.   right side up
C.   sideways
D.   upside down
E.   none of the above is correct

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