Bio 2010 - Microbiology » Summer 2021 » Aseptic Transfer Technique

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Question #1
How should you sterilize materials that will make contact with your culture media?
A.   you don't need to sterilize materials that will make contact with your experiment
B.   wiping it down with a tissue
C.   more than one of the above is correct
D.   by using the Bunsen burner and flaming them
E.   wash them in the sink
F.   none of the above is correct
Question #2
What is the name of the instrument that we MUST use during the aseptic microorganism transfer during our lab activities?
A.   flame loop
B.   pipettor
C.   flame wand
D.   finger
Question #3
Which of the following would be the BEST time to label test tubes and Petri plates?
A.   more than one of the above is correct
B.   before you use them
C.   while you are using them
D.   after using them
E.   none of the above is correct
Question #4
You and your partner were talking during your aseptic transfer activity. You cannot remember if you flamed your flame loop before you opened your first test tube. What should you do in order to avoid contamination?
A.   nothing, the last person flamed it
B.   nothing, bacteria cannot grow on the metal loop
C.   flame it again just to be sure.
D.   get a different flame loop
Question #5
Once inoculated with bacteria, how should agar Petri plates be incubated?
A.   sideways
B.   right side up
C.   more than one of the above is correct
D.   upside down
E.   none of the above is correct

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