Bio 2010 - Microbiology » Summer 2021 » Aseptic Transfer Technique

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Question #1
How should you sterilize materials that will make contact with your culture media?
A.   you don't need to sterilize materials that will make contact with your experiment
B.   more than one of the above is correct
C.   wash them in the sink
D.   by using the Bunsen burner and flaming them
E.   wiping it down with a tissue
F.   none of the above is correct
Question #2
What is the name of the instrument that we MUST use during the aseptic microorganism transfer during our lab activities?
A.   pipettor
B.   flame loop
C.   finger
D.   flame wand
Question #3
Which of the following would be the BEST time to label test tubes and Petri plates?
A.   before you use them
B.   while you are using them
C.   more than one of the above is correct
D.   after using them
E.   none of the above is correct
Question #4
You and your partner were talking during your aseptic transfer activity. You cannot remember if you flamed your flame loop before you opened your first test tube. What should you do in order to avoid contamination?
A.   nothing, bacteria cannot grow on the metal loop
B.   get a different flame loop
C.   nothing, the last person flamed it
D.   flame it again just to be sure.
Question #5
Once inoculated with bacteria, how should agar Petri plates be incubated?
A.   upside down
B.   more than one of the above is correct
C.   right side up
D.   sideways
E.   none of the above is correct

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