Bio 2010 - Microbiology » Summer 2021 » Gram Stain Technique

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Question #1
Which of the following is the Gram's primary stain?
A.   Safranin
B.   Crystal violet
C.   Acetone- Alcohol
D.   Carbolfuchsin
E.   Loeffler's methylene blue
Question #2
What cellular structure cause the different results after Gram staining?
A.   Flagella
B.   Cell wall
C.   Cell membrane
D.   Cytoplasm
E.   Mycolic acid
Question #3
Why is it important that you don’t put too much water to prepare the smear?
A.   It will cause the cells to swim away
B.   It will take too long to dry the smear
C.   Water may carry contaminants
D.   Water can dilute the stain
Question #4
Which of the following are the correct order of chemicals used in Gram staining?
A.   Iodine, Acetone-alcohol, crystal violet, safranin
B.   Safranin, crystal violet, iodine, acetone-alcohol
C.   Crystal violet, iodine, safranin, acetone-alcohol
D.   Crystal violet, iodine, acetone-alcohol, safranin

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