Bio 2010 - Microbiology » Summer 2021 » Gram Stain Technique

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Question #1
Which of the following is the Gram's primary stain?
A.   Safranin
B.   Acetone- Alcohol
C.   Crystal violet
D.   Loeffler's methylene blue
E.   Carbolfuchsin
Question #2
What cellular structure cause the different results after Gram staining?
A.   Cell wall
B.   Flagella
C.   Cytoplasm
D.   Mycolic acid
E.   Cell membrane
Question #3
Why is it important that you don’t put too much water to prepare the smear?
A.   Water may carry contaminants
B.   It will take too long to dry the smear
C.   It will cause the cells to swim away
D.   Water can dilute the stain
Question #4
Which of the following are the correct order of chemicals used in Gram staining?
A.   Safranin, crystal violet, iodine, acetone-alcohol
B.   Iodine, Acetone-alcohol, crystal violet, safranin
C.   Crystal violet, iodine, acetone-alcohol, safranin
D.   Crystal violet, iodine, safranin, acetone-alcohol

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