Bio 2010 - Microbiology » Summer 2021 » Pour Plate Technique

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Question #1
Which of the following best describes why we do not put the lid on the bench top when pouring our agar?
A.   more than one of the above is correct
B.   the lid cold become contaminated by microorganisms on the desk
C.   it takes up too much space and we may knock it onto the ground
D.   the lifting the lid at approximately 45° limits agar surface contaminants
E.   none of the above is correct
Question #2
Which of the following is agar's melting temperature?
A.   40°C
B.   100°C
C.   45°C
D.   50°C
E.   none of the above is correct
Question #3
In our discussions, we described how to make a proper label and placing the label on the bottom of our pour plate petri dish. Which of the following BEST describes why we place labels on the bottom of the Petri dish?
A.   this is preference, it really does not matter if the label is on the plate bottom or top
B.   more than one of the above is correct
C.   if the plates are dropped and scatter on the floor, the label remains with the agar side of the plastic plate
D.   it makes it harder for the bacteria to escape from the plate
E.   none of the above is correct
Question #4
Suppose you obtained your tube with melted agar, brought it back to your desk as a melted liquid, and subsequently forgot about the tube. When you remember, you look and discover the agar in the tube has solidified. Which of the following BEST describes why the agar changed from liquid to solid?
A.   the technician did not turn the water bath on
B.   the agar temperature dropped from 50°C to 40°C (or below)
C.   more than one of the above is correct
D.   your lab partner switched out the tubes
E.   It was old agar and it did not perform as expected
F.   none of the above is correct
Question #5
Which of the following temperatures does agar solidify?
A.   40°C
B.   100°C
C.   45°C
D.   50°C
E.   none of the above is correct

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