Chem 40005 - Clinical Chemistry » Summer 2021 » Module 2 Quiz

Need help with your exam preparation?

Question #1
A commonly used electrode also used as an internal reference electrode in ion-selective electrode measurements is which one of the following?
A.   Silver/silver chloride
B.   Gas permeable
C.   Mercury vapor
D.   Platinum
Question #2
The light source in an atomic absorption spectrophotometer is typically a
A.   prism.
B.   hollow cathode tube.
C.   laser light.
D.   tungsten light.
Question #3
Incorporation of _____, a neutral antibiotic, into a polyvinyl chloride membrane allows for the manufacture of an ion-selective electrode that is highly selective for potassium.
A.   tetracycline
B.   nonactin
C.   erythromycin
D.   valinomycin
Question #4
What is the importance of the ionic strength of the buffer used in gel electrophoresis?
A.   A heat-labile protein will become denatured if the ionic strength of the buffer is too high.
B.   Increased ionic strength will lead to a backward electrophoretic pattern.
C.   Staining of the protein fractions will not occur if the ionic strength in the buffer is too high.
D.   No migration will occur if a buffer’s ionic strength is too high.
Question #5
What stains are used to stain a serum protein electrophoresis gel?
A.   Crystal Violet and Sudan Black
B.   Coomassie Blue and ethidium bromide
C.   Oil Red O and Amido Black
D.   Amido Black and Coomassie Brilliant Blue
Question #6
Which two optical techniques are combined operationally in a flow cytometer?
A.   Spectrophotometry and fluorescence polarization techniques
B.   Fluorescence polarization and laser-induced fluorometry
C.   Atomic absorption spectrophotometry and light scattering techniques
D.   Laser-induced fluorometry and light scattering techniques
Question #7
A separation technique that can detect proteins in a complex mixture with the use of antibodies directed against a protein of interest is
A.   capillary electrophoresis.
B.   Western blotting.
C.   Northern blotting.
D.   electrokinetic chromatography.
Question #8
An electrochemical technique used to measure chloride in serum or plasma is
A.   coulometry.
B.   conductometry.
C.   amperometry.
D.   voltammetry.
Question #9
When selecting a photometric technique that is more sensitive to a low concentration of analyte, a laboratory director compares absorbance measuring devices against those that measure fluorescence intensity. Which technique is considered more sensitive in its measurements and why?
A.   Absorbance measurements are more sensitive because the flame can break down complex compounds into elements that are specifically measured.
B.   Fluorescence intensity measurements are more sensitive because the fluorophores used are very specific to the analytes they bind to and will bind to small analytes easily.
C.   Fluorescence intensity measurements are more sensitive because of the use of intense light sources, signal filtering, and sensitive light emission photometers.
D.   Absorbance measurements are more sensitive because of the ability of the monochromator to isolate very specific parts of the spectrum.
Question #10
In capillary electrophoresis, hydrodynamic sample injection
A.   involves sample injection into the capillary by applying a voltage.
B.   is best for analytes that have a higher electrophoretic mobility.
C.   is sometimes considered to be a “biased” type of sample injection.
D.   involves sample injection into the capillary by applying a positive pressure to the sample inlet.
Question #11
The isoelectric point (pI) of a protein is the pH at which the protein
A.   has a positive charge.
B.   has a negative charge.
C.   has no net charge.
D.   migrates best in electrophoresis.
Question #12
A biosensor that uses both enzyme detection and an ion-specific electrode could be examining
A.   cholesterol using cholesterol oxidase with production of fluorescent cations.
B.   glucose using glucose oxidase and the production of biocarbonate ions.
C.   urea using urease and the production of ammonium ions.
D.   bilirubin using bilirubin oxidase and the production of hydrogen ions.
Question #13
Measurement of pH involves which kind of electrode?
A.   Hydrogen redox electrode
B.   Hydrogen ion-selective electrode
C.   Voltammetric hydrogen electrode
D.   Gas permeable PCO2 electrode
Question #14
Upon receiving a serum sample for protein electrophoresis, which you perform using agarose gel, you note that the specimen is hemolyzed. How would hemolysis affect the banding pattern of this type of electrophoresis?
A.   There would be an increased β-globulin band.
B.   There would be no effect on any of the protein bands.
C.   There would be an increased α1-globulin band.
D.   There would be an increase in the γ-globulin bands.
Question #15
Which one of the following optical techniques is not considered an emission technique?
A.   Fluorometry
B.   Luminometry
C.   Nephelometry
D.   Phosphorimetry
Question #16
The main advantage of capillary electrophoresis over standard gel electrophoresis is
A.   the small sample size required.
B.   the increased amount of time required for separation.
C.   the ability to use a variety of buffers in the system.
D.   more efficient heat dissipation for better separation.
Question #17
Potentiometric measurements are based on galvanic electrochemical cells. These types of cells consist of
A.   two electron or metallic conducting electrodes that are connected by an electrolyte solution that conducts ions.
B.   a biologic recognition element and a physicochemical transducer, often an electrochemical or optical device.
C.   a polarizable working electrode to which an external voltage is applied with the resulting cathodic or anodic current of the cell being monitored.
D.   two inert metal electrodes to which an electrical potential is applied.
Question #18
A molecule is said to fluoresce when it absorbs light at one wavelength and emits light of
A.   the same wavelength.
B.   a shorter wavelength.
C.   a lower energy.
D.   a higher energy.
Question #19
A physician is concerned about an emphysema patient’s PO2 level and wants to determine it in this patient’s blood. Which one of the following would be best used to determine PO2?
A.   Ion-selective electrode
B.   Amperometric method
C.   Coulometric method
D.   Conductivity-based measurement
Question #20
What would the concentration of albumin be if the total protein value was 8.5 g/dL and the densitometer measured the albumin fraction as 55% of the total?
A.   0.065 g/dL
B.   Albumin values cannot be calculated using densitometry.
C.   4.68 g/dL
D.   15.4 g/dL

Need help with your exam preparation?