Bio 2010 - Microbiology » Summer 2021 » Chapter 4 Quiz

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Question #1
A compound light microscope allows clear visualization of objects no smaller than a:
A.   four leaf clover
B.   small bacterium
C.   human cell
D.   typical virus
E.   DNA molecule
Question #2
The kind of electron microscope which is used to study internal structure of cells is:
A.   light microscope
B.   compound microscope
C.   scanning electron microscope
D.   transmission electron microscope
Question #3
Which of the following has the BEST resolution?
A.   Phase contrast
B.   bright field microscopy
C.   differential interference contrast (DIC)
D.   dark field microscopy
E.   none of the above
Question #4
Light of ____ wavelength typically will result in ____ resolving power.
A.   shorter, worse
B.   shorter, better
C.   any, poor
D.   longer, better
Question #5
The transmission electron microscope has the greatest resolving power because it uses an electron beam to view the sample instead of a light beam. The electron beam is used because:
A.   Electrons have shorter wavelengths than light waves
B.   Electrons do not penetrate the sample.
C.   Electrons are less invasive
D.   Light waves are less visible.
Question #6
You want to determine the location of a specific protein in a cell, using a color stain. Which of the following is the BEST type of microscopy to use?
A.   bright field microscopy
B.   phase contrast microsocpy
C.   fluorescent microscopy
D.   dark field microscopy
E.   electron microscopy
Question #7
Phase Contrast microscopy is different than bright field microscopy because:
A.   Uses filters in the objective to give contrast to parts of the cell with different thicknesses
B.   Continuously changes the phase of the incident light from the condenser to improve contrast in the specimen.
C.   Uses special lenses to change the color of light passing through them.
D.   Uses special lenses to distinguish between solid and liquid phases of the cell
Question #8
Which of the following is used to visualize live cells?
A.   TEM
B.   SEM
C.   Phase Contrast
D.   SEM, TEM, and Phase Contrast
Question #9
Which of the following microscope types has the best contrast?
A.   bright field miscroscopy
B.   phase contrast
C.   differential interference contrast (DIC)
D.   dark field miscroscopy
Question #10
A microscope that exposes specimens to ultraviolet light and forms an image with the light emitted at a different wavelength is called a __________ microscope.
A.   phase contrast
B.   darkfield
C.   fluorescent
D.   scanning electron

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