Bio 2010 - Microbiology » Summer 2021 » Pour Plate Technique (A)

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Question #1
Which of the best describes the purpose of the Pour Plate technique?
A.   to pour melted agar onto the top short-Petri dish wall
B.   to pour melted agar into slanted test tubes
C.   to pour melted agar onto microscope slides
D.   to pour melted agar onto the bottom wide-Petri dish wall
Question #2
In our discussions, we described how to make a proper label and placing the label on the bottom of our pour plate petri dish. Which of the following BEST describes why we place labels on the bottom of the Petri dish?
A.   if the plates are dropped and scatter on the floor, the label remains with the agar side of the plastic plate
B.   it makes it harder for the bacteria to escape from the plate
C.   this is preference, it really does not matter if the label is on the plate bottom or top
D.   more than one of the above is correct
Question #3
After our lab technician makes agar in test tubes, she places the agar tubes in a hot water bath, at 50°C. Which of the following describes why she leaves them in the hot water bath at this temperature?
A.   it is the pouring temperature
B.   more than one of the above is correct
C.   it is the holding temperature
D.   it is the melting temperature
E.   it is the temperature to solidify
Question #4
Suppose you obtained your tube with melted agar, brought it back to your desk, and subsequently forgot about the tube. When you remember, you look and discover the agar in the tube has solidified. Your lab partner says, just put it in a beaker of hot water, it will melt again. You try this with no success. The agar did not melt. Which of the following best explains why the agar did not melt?
A.   the water temperature was not hot enough
B.   the water temperature was not cool enough
C.   agar cannot be re-melted
D.   cooling the agar it destroyed it
Question #5
Which of the following is agar's pouring temperature?
A.   50°C
B.   40°C
C.   100°C
D.   45°C

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