Bio 2010 - Microbiology » Summer 2021 » Aseptic Transfer Technique (B)

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Question #1
Which of the following best describes sterile media?
A.   more than one of the above is correct
B.   media free from living organisms
C.   media free from nutrients
D.   media with low pH
E.   none of the above is correct
Question #2
You and your partner were talking during your aseptic transfer activity. You cannot remember if you flamed your flame loop before you opened your first test tube. What should you do in order to avoid contamination?
A.   nothing, bacteria cannot grow on the metal loop
B.   get a different flame loop
C.   flame it again just to be sure.
D.   nothing, the last person flamed it
Question #3
Why is it important to flame the test tubes before and after transferring bacteria into fresh, sterile culture media?
A.   to seal the plastic lid on the tube
B.   prevent contaminating the second culture tube
C.   more than one of the above is correct
D.   clean the tube for use in the future
E.   none of the above is correct
Question #4
Where should Petri dish lids be placed during the aseptic transfer technique?
A.   on the table, facing down
B.   in your hand, facing up
C.   more than one of the above is correct
D.   on the table, facing up
E.   in your hand, facing down
F.   none of the above is correct
Question #5
What is the goal of aseptic transfer technique?
A.   to allow contamination
B.   to prevent contamination
C.   more than one of the above is correct
D.   to reduce contamination
E.   none of the above is correct

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