Bio 2010 - Microbiology » Summer 2021 » Aseptic Transfer Technique (B)

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Question #1
Which of the following best describes sterile media?
A.   media free from nutrients
B.   media free from living organisms
C.   media with low pH
D.   more than one of the above is correct
E.   none of the above is correct
Question #2
You and your partner were talking during your aseptic transfer activity. You cannot remember if you flamed your flame loop before you opened your first test tube. What should you do in order to avoid contamination?
A.   flame it again just to be sure.
B.   get a different flame loop
C.   nothing, the last person flamed it
D.   nothing, bacteria cannot grow on the metal loop
Question #3
Why is it important to flame the test tubes before and after transferring bacteria into fresh, sterile culture media?
A.   more than one of the above is correct
B.   to seal the plastic lid on the tube
C.   clean the tube for use in the future
D.   prevent contaminating the second culture tube
E.   none of the above is correct
Question #4
Where should Petri dish lids be placed during the aseptic transfer technique?
A.   on the table, facing up
B.   more than one of the above is correct
C.   on the table, facing down
D.   in your hand, facing down
E.   in your hand, facing up
F.   none of the above is correct
Question #5
What is the goal of aseptic transfer technique?
A.   to allow contamination
B.   to prevent contamination
C.   more than one of the above is correct
D.   to reduce contamination
E.   none of the above is correct

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