Bio 2010 - Microbiology » Summer 2021 » Pour Plate Technique (B)

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Question #1
Suppose you obtained your tube with melted agar, brought it back to your desk as a melted liquid, and subsequently forgot about the tube. When you remember, you look and discover the agar in the tube has solidified. Which of the following BEST describes why the agar changed from liquid to solid?
A.   It was old agar and it did not perform as expected
B.   your lab partner switched out the tubes
C.   the agar temperature dropped from 50°C to 40°C (or below)
D.   more than one of the above is correct
E.   the technician did not turn the water bath on
F.   none of the above is correct
Question #2
Suppose you obtained your tube with melted agar, brought it back to your desk, and subsequently forgot about the tube. When you remember, you look and discover the agar in the tube has solidified. Your lab partner says, just put it in a beaker of hot water, it will melt again. You try this with no success. The agar did not melt. Which of the following best explains why the agar did not melt?
A.   the water temperature was not hot enough
B.   more than one of the above is correct
C.   cooling the agar it destroyed it
D.   the water temperature was not cool enough
E.   agar cannot be re-melted
F.   none of the above is correct
Question #3
Which of the following is agar's melting temperature?
A.   50°C
B.   40°C
C.   100°C
D.   45°C
E.   none of the above is correct
Question #4
Which of the following is agar's holding temperature?
A.   45°C
B.   50°C
C.   40°C
D.   100°C
E.   none of the above is correct
Question #5
Which of the best describes the purpose of the Pour Plate technique?
A.   to pour melted agar onto microscope slides
B.   to pour melted agar onto the bottom wide-Petri dish wall
C.   to pour melted agar onto the top short-Petri dish wall
D.   to pour melted agar into slanted test tubes

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