Bio 2010 - Microbiology » Summer 2021 » Streak For Isolation Technique (B)

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Question #1
Which of the following sectors/quadrants of our Streak-for-Isolation plate should have ISOLATED COLONIES growing in it?
A.   II
B.   III
C.   0
D.   I
Question #2
Which of the following would be considered a next step application for any isolated colonies on your streak-for-isolation plate?
A.   inoculation
B.   inspection
C.   more than one of the above is correct
D.   incubation inspection
E.   identification
F.   none of the above is correct
Question #3
Which of the following observations would suggest that a plate was inoculated with a pure culture?
A.   bacterial growth is apparent in all four quadrants.
B.   isolated colonies have uniform color and same size.
C.   isolated colonies are all white in color, but some colonies are noticeably larger than others.
D.   bacterial growth is apparent along the streaks connecting each quadrant.
Question #4
After each streak on the plate with the inoculating loop, what is done to the loop?
A.   wiped with disinfectant
B.   re-introduced to stock culture
C.   flamed until loop glows
D.   discarded
Question #5
A student in our class makes their first Streak-for-Isolation plate and has it returned to them after incubation. Looking at it, you notice that there is bacterial growth in the first two quadrants/sectors, but not in the last two quadrants/sectors. Which of the following is the most likely explanation for this observation?
A.   they forgot to flame their loop before making the next sector/quadrant
B.   they did not incubate the agar plate at the ideal temperature
C.   they did not make contact in the second sector/quadrant while making the third sector/quadrant.
D.   they did not incubate the agar plate long enough

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