Bio 2010 - Microbiology » Summer 2021 » Aseptic Transfer Technique (C)

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Question #1
Which of the following best describes contamination?
A.   presence of oxygen
B.   presence of carbon dioxide
C.   oxygen free
D.   more than one of the above is correct
E.   presence of microorganisms
F.   none of the above is correct
Question #2
Which of the following would be the BEST time to label test tubes and Petri plates?
A.   more than one of the above is correct
B.   while you are using them
C.   before you use them
D.   after using them
E.   none of the above is correct
Question #3
You and your partner were talking during your aseptic transfer activity. You accidentally touch your hand with the flame loop while waiting for it to cool down. Fortunately, it the loop was cool to the touch and you did not burn yourself. What should you do in order to avoid contaminating the sterile media tube in your right hand?
A.   start over by re-flaming the loop
B.   nothing, I showered that morning, my hand should be sterile
C.   get a different flame loop
D.   nothing, bacteria cannot grow on the metal loop after it cools down
Question #4
Once inoculated with bacteria, how should agar Petri plates be incubated?
A.   right side up
B.   sideways
C.   upside down
D.   more than one of the above is correct
E.   none of the above is correct
Question #5
Which of the following best describes sterile media?
A.   media free from living organisms
B.   media free from nutrients
C.   media with low pH
D.   more than one of the above is correct
E.   none of the above is correct

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