Bio 2010 - Microbiology » Summer 2021 » Aseptic Transfer Technique (C)

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Question #1
Which of the following best describes contamination?
A.   more than one of the above is correct
B.   presence of oxygen
C.   presence of microorganisms
D.   oxygen free
E.   presence of carbon dioxide
F.   none of the above is correct
Question #2
Which of the following would be the BEST time to label test tubes and Petri plates?
A.   while you are using them
B.   after using them
C.   before you use them
D.   more than one of the above is correct
E.   none of the above is correct
Question #3
You and your partner were talking during your aseptic transfer activity. You accidentally touch your hand with the flame loop while waiting for it to cool down. Fortunately, it the loop was cool to the touch and you did not burn yourself. What should you do in order to avoid contaminating the sterile media tube in your right hand?
A.   nothing, bacteria cannot grow on the metal loop after it cools down
B.   get a different flame loop
C.   start over by re-flaming the loop
D.   nothing, I showered that morning, my hand should be sterile
Question #4
Once inoculated with bacteria, how should agar Petri plates be incubated?
A.   sideways
B.   upside down
C.   more than one of the above is correct
D.   right side up
E.   none of the above is correct
Question #5
Which of the following best describes sterile media?
A.   more than one of the above is correct
B.   media free from nutrients
C.   media free from living organisms
D.   media with low pH
E.   none of the above is correct

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