Bio 2010 - Microbiology » Summer 2021 » Aseptic Transfer Technique (C)

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Question #1
Which of the following best describes contamination?
A.   presence of carbon dioxide
B.   presence of microorganisms
C.   more than one of the above is correct
D.   presence of oxygen
E.   oxygen free
F.   none of the above is correct
Question #2
Which of the following would be the BEST time to label test tubes and Petri plates?
A.   before you use them
B.   more than one of the above is correct
C.   after using them
D.   while you are using them
E.   none of the above is correct
Question #3
You and your partner were talking during your aseptic transfer activity. You accidentally touch your hand with the flame loop while waiting for it to cool down. Fortunately, it the loop was cool to the touch and you did not burn yourself. What should you do in order to avoid contaminating the sterile media tube in your right hand?
A.   get a different flame loop
B.   start over by re-flaming the loop
C.   nothing, bacteria cannot grow on the metal loop after it cools down
D.   nothing, I showered that morning, my hand should be sterile
Question #4
Once inoculated with bacteria, how should agar Petri plates be incubated?
A.   more than one of the above is correct
B.   upside down
C.   sideways
D.   right side up
E.   none of the above is correct
Question #5
Which of the following best describes sterile media?
A.   media free from living organisms
B.   media free from nutrients
C.   media with low pH
D.   more than one of the above is correct
E.   none of the above is correct

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