Bio 2010 - Microbiology » Summer 2021 » Streak for Isolation Technique (C)

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Question #1
Which of the following observations would suggest that a plate was inoculated with a pure culture?
A.   bacterial growth is apparent along the streaks connecting each quadrant.
B.   bacterial growth is apparent in all four quadrants.
C.   isolated colonies have uniform color and same size.
D.   isolated colonies are all white in color, but some colonies are noticeably larger than others.
Question #2
A student in our class makes their first Streak-for-Isolation plate and has it returned to them after incubation. Looking at it, you notice that there is bacterial growth in the first two quadrants/sectors, but not in the last two quadrants/sectors. Which of the following is the most likely explanation for this observation?
A.   they forgot to flame their loop before making the next sector/quadrant
B.   they did not make contact in the second sector/quadrant while making the third sector/quadrant.
C.   they did not incubate the agar plate long enough
D.   they did not incubate the agar plate at the ideal temperature
Question #3
Where should you label your Petri plate with information about the culture?
A.   on the inside of the lid.
B.   on the side of the bottom (agar-containing) portion of the plate.
C.   on the bottom of the plate.
D.   on the top of the lid.
Question #4
Which of the following sectors/quadrants of our Streak-for-Isolation plate should have CONFLUENT bacterial growth?
A.   I
B.   II
C.   III
D.   0
Question #5
What does the streak technique enable us to achieve?
A.   visualization of virus plaques
B.   development of antibacterial microbes
C.   direct spread and dilution of bacteria from a single colony
D.   isolation of bacterial colonies

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