Biol 303 - Intermediate Cell Biology » Fall 2021 » Post Quiz Journal Article 1

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Question #1
What technique was used in addition to western blot analysis to further prove that ABCB6 was located in the lysosomes of K562 cells?
A.   Flow cytometry
B.   Confocal Microscopy
C.   Exosome Isolation
D.   Differential centrifugation
Question #2
What happens to the level of hemoglobin as related to the amount of ABCB6 when differentiation was chemically induced in K562 cells?
A.   The level of hemoglobin increased as did the amount of ABCB6.
B.   The increased level of hemoglobin correlated to a decrease in ABCB6 protein.
C.   The level of hemoglobin remained the same as the control and did not correlate with the increased amount of ABCB6.
D.   The level of hemoglobin decreased, as did the amount of ABCB6.
Question #3
In the paper, confocal microscopy showed ABCB6 to be colocalized with which marker of exosomes?
A.   Tfr
B.   Tsg 101
C.   Spectrin
D.   Flotillin
E.   Alix
Question #4
Differential centrifugation produces different cellular fractions based on the length of time the cell lysate is centrifuged.
A.   TRUE
B.   FALSE
Question #5
The authors of this paper (Kiss et al.) proposed that ABCB6, unlike the three other ABC transporters (7, 8 and 10), was not localized to mitochondria within the cell.
A.   TRUE
B.   FALSE
Question #6
The purpose of figure 3A was to
A.   Test four different ABCB6 antibodies; some of which were commerically made and others custom made and show that porin was not located in the membranes of red blood cell ghosts.
B.   Provide evidence that overexpression of HA-tagged ABCB6 in K562 cells was not identified using the ABCB6 antibodies and show that porin was not located in the membranes of red blood cell ghosts.
C.   Show that treatment of RBC and K562 cells overexpressing ABCB6 with PNGase F proved ABCB6 is expressed as a N-glycosylated protein.
D.   Provide evidence that overexpression of HA-tagged ABCB6 in K562 cells was not identified using the ABCB6 antibodies and test four different ABCB6 antibodies; some of which were commerically made and others custom made.
Question #7
The majority of ABCB6 expression was identified in exosomes from mature red blood cells, rather than from reticulocytes.
A.   FALSE
B.   TRUE
Question #8
What role does B-actin play in this paper (used in figure 5)?
A.   Its expression is upregulated during erythroid differentiation.
B.   It is not influenced by erythroid differentiation and is used as a loading control for the western blots.
C.   It colocalizes with ABCB6 and increases with increasing expression of ABCB6.
D.   It is used as to measure increased expression of PPIX-fluorescence.
Question #9
In figure 5 they induced erythroid differentiation. What does that do to the expression of ABCB6?
A.   It upregulates ABCB6 protein expression.
B.   It downregulates ABCB6 protein expression.
C.   ABCB6 protein expression is not affected by erythroid differentiation.
D.   ABCB6 expression is correlated with PPIX fluorescence.
Question #10
Colocalization as identified by confocal microscopy is represented by a different color. For example, if red and green fluorescence colocalizes, the resulting color is yellow.
A.   FALSE
B.   TRUE
Question #11
Why is it important to known where ABCB6 is located in the cell?
A.   Knowing the location of ABCB6 in the cell would show the location of all the other ABC family members.
B.   The location proves that ABCB6 plays a role in transporting solutes across mitochondrial membranes.
C.   Localization of ABCB6 to a specific region of the RBC shows it is important in heme biosynthesis.
D.   Understanding where the ABCB6 transporter is located leads to answering the question of what its function is.
Question #12
In this journal article, the authors provide multiple pieces of evidence to prove their hypothesis. Which of the statements below was NOT proved by the data in the paper?
A.   ABCB6 is glycosylated, and glycosylated proteins are very rarely found in mitochondria.
B.   ABCB6 colocalizes with markers for the endolysosomal compartment.
C.   ABCB6 is expressed in plasma membranes of erythrocytes.
D.   Silencing of ABCB6 using shRNA interfered with the ability of K562 cells to undergo differentiation.

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