Biol 303 - Intermediate Cell Biology » Fall 2021 » Pre Quiz Journal Article 1

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Question #1
The paper has proven which of the following ideas/hypotheses:
A.   ABCB6 can be identified in exosomes of red blood cells and glycosylation of ABCB6 protein directs it to the mitochondria
B.   ABCB6 can be identified in exosomes of red blood cells, ABCB6 protein is found in the plasma membrane of red blood cells and the location of three other ABC proteins (7, 8 and 10), was confirmed to be localized to the mitochondria membrane
C.   ABCB6 appears to be responsible for mitochondria uptake of porphyrins and glycosylation of ABCB6 protein directs it to the mitochondria
Question #2
Which of the following experiments showed how ABCB6 is not involved in heme biosynthesis?
A.   Figure 5 showing porphyrin synthesis and ABCB6 expression in K562 cells
B.   Figure 3 showing ABCB6 expression in mature erythrocytes
C.   Figure S4 detecting ABCB6 in secreted reticulocyte exosomes
D.   Figure 4 showing ABCB6 staining associated with human reticulocytes
Question #3
What was the purpose of using PNGase F?
A.   To prevent transmembrane proteins from embedding in the plasma membrane
B.   To break apart the plasma membrane
C.   To remove N-linked oligosaccharides from glycoproteins
D.   To add N-linked oligosaccharides to glycoproteins
Question #4
What cell components are missing from the red blood cell ghosts
A.   nucleus, hemoglobin and mitochondria
B.   plasma membrane and hemoglobin
C.   plasma membrane and nucleus
Question #5
Most human ABC proteins can be found in multiple areas of the cell including the plasma membrane, lysosomes, and mitochondria.
A.   TRUE
B.   FALSE
Question #6
What do shRNAs do?
A.   They increase gene expression.
B.   They degrade the protein that is being targeted.
C.   They silence gene expression
D.   They base pair with complementary sequences as a probe.

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