Microbiology 020 - General Microbiology » Fall 2021 » Lab Quiz 2 Acid Fast Stain

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Question #1
The lung X-ray of a patient shows many lesions that are likely filled with mucous and pus from some type of infection. The patient is coughing and has coughed up blood. The cough has lasted several months prior to the patient coming into the emergency room for help. As part of the workup, you do an acid-fast stain on the patients sputum. You find acid-fast bacteria. You may diagnose the patient with ___________.
A.   Tuberculosis
B.   There is not enough data to make a diagnosis.
C.   COVID-19
D.   Influenza
E.   Common cold
Question #2
What is the primary stain for the acid-fast stain?
A.   Safranin
B.   Iodine
C.   The correct answer is not listed here.
D.   Carbol fuschin
E.   Crystal violet
Question #3
The acid fast stain may be used to positively identify members of the genus ___________.
A.   Staphylococcus
B.   The correct answer is not listed here.
C.   Enterococcus
D.   Bacillus
E.   Mycobacterium
Question #4
Cells that appear pink after an acid-fast stain have ________ in its cell wall.
A.   Mycolic acids
B.   Thin layer of peptidoglycan
C.   No peptidoglycan
D.   Thick layer of peptidoglycan
Question #5
The lung X-ray of a patient shows many lesions that are likely filled with mucous and pus from some type of infection. The patient is coughing and has coughed up blood. The cough has lasted several months prior to the patient coming into the emergency room for help. As part of the workup, you do an acid-fast stain on the patients sputum. You find acid-fast bacteria. You may diagnose the patient with ___________.
A.   There is not enough data to make a diagnosis.
B.   Tuberculosis
C.   Common cold
D.   COVID-19
E.   Influenza
Question #6
What is the counterstain for the acid-fast stain?
A.   Methylene blue
B.   Carbol fuschin
C.   Malachite green
D.   Iodine
E.   Crystal violet
F.   Safranin
Question #7
What is the de-stain for the Acid-Fast stain?
A.   Acid-alcohol
B.   Water
C.   Alcohol
D.   Iodine
Question #8
Put the following in the correct order. I. Methylene blue II. Carbol fuschin III. Acid-alcohol IV. Several rounds of heating until steaming
A.   II, IV, III, I
B.   II, III, IV, I
C.   I, III, II, IV
D.   I, II, III, IV
Question #9
Which of the following stains requires heating during the staining procedure?
A.   Gram stain
B.   All the stains listed here require heating during the staining procedure.
C.   Acid-fast stain
D.   Simple stain
Question #10
Put the following in the correct order. I. Methylene blue II. Carbol fuschin III. Acid-alcohol IV. Several rounds of heating until steaming
A.   I, III, II, IV
B.   II, III, IV, I
C.   II, IV, III, I
D.   I, II, III, IV

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