Microbiology 020 - General Microbiology » Fall 2021 » Lab Quiz 2 Acid Fast Stain

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Question #1
The lung X-ray of a patient shows many lesions that are likely filled with mucous and pus from some type of infection. The patient is coughing and has coughed up blood. The cough has lasted several months prior to the patient coming into the emergency room for help. As part of the workup, you do an acid-fast stain on the patients sputum. You find acid-fast bacteria. You may diagnose the patient with ___________.
A.   Common cold
B.   Tuberculosis
C.   Influenza
D.   There is not enough data to make a diagnosis.
E.   COVID-19
Question #2
What is the primary stain for the acid-fast stain?
A.   Carbol fuschin
B.   Crystal violet
C.   Safranin
D.   Iodine
E.   The correct answer is not listed here.
Question #3
The acid fast stain may be used to positively identify members of the genus ___________.
A.   Bacillus
B.   Enterococcus
C.   Mycobacterium
D.   The correct answer is not listed here.
E.   Staphylococcus
Question #4
Cells that appear pink after an acid-fast stain have ________ in its cell wall.
A.   No peptidoglycan
B.   Thick layer of peptidoglycan
C.   Mycolic acids
D.   Thin layer of peptidoglycan
Question #5
The lung X-ray of a patient shows many lesions that are likely filled with mucous and pus from some type of infection. The patient is coughing and has coughed up blood. The cough has lasted several months prior to the patient coming into the emergency room for help. As part of the workup, you do an acid-fast stain on the patients sputum. You find acid-fast bacteria. You may diagnose the patient with ___________.
A.   Common cold
B.   COVID-19
C.   Influenza
D.   There is not enough data to make a diagnosis.
E.   Tuberculosis
Question #6
What is the counterstain for the acid-fast stain?
A.   Malachite green
B.   Carbol fuschin
C.   Crystal violet
D.   Methylene blue
E.   Iodine
F.   Safranin
Question #7
What is the de-stain for the Acid-Fast stain?
A.   Alcohol
B.   Iodine
C.   Acid-alcohol
D.   Water
Question #8
Put the following in the correct order. I. Methylene blue II. Carbol fuschin III. Acid-alcohol IV. Several rounds of heating until steaming
A.   II, IV, III, I
B.   I, II, III, IV
C.   I, III, II, IV
D.   II, III, IV, I
Question #9
Which of the following stains requires heating during the staining procedure?
A.   Gram stain
B.   All the stains listed here require heating during the staining procedure.
C.   Simple stain
D.   Acid-fast stain
Question #10
Put the following in the correct order. I. Methylene blue II. Carbol fuschin III. Acid-alcohol IV. Several rounds of heating until steaming
A.   II, IV, III, I
B.   I, III, II, IV
C.   I, II, III, IV
D.   II, III, IV, I

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