Microbiology 020 - General Microbiology » Fall 2021 » Lab Quiz 5 Battery Tests

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Question #1
If bacteria in TSIA ferment sugars, _______ turns _______.
A.   Methyl red; red
B.   Phenol red indicator; red
C.   Methyl red; yellow
D.   Phenol red indicator; yellow
E.   Phenol red indicator; black
Question #2
Sulfur reduction in TSIA appears as _______.
A.   Gas formation
B.   Yellow throughout
C.   Black precipitate in the butt
D.   Black precipitate in the slant
Question #3
TSIA stands for ____.
A.   Triple sugar iron agar
B.   Total sucrose inositol agar
C.   Total sugar iron agar
Question #4
After inoculation and incubation, a SIM test appears very cloudy. This indicates _______.
A.   more bacteria should have been inoculated into the test.
B.   the bacterium is motile.
C.   the bacterium produces tryptophanase.
D.   the bacterium is non-motile.
E.   the test was done incorrectly.
Question #5
Is SIM selective, differential, both, or neither?
A.   Selective & differential
B.   Differential only
C.   Selective only
D.   None.
Question #6
How is the SIM test inoculated?
A.   Stab the anaerobic region and streak the aerobic region.
B.   Spread the bacteria over the medium with a sterile swab.
C.   Mix in 1 loopful of bacteria from an overnight broth culture.
D.   Stab the medium in a straight line.
Question #7
A lab scientist has conducted an IMVC test from bacteria isolated from a sick patient. The scientist is likely attempting to determine if the patient is infected by ______.
A.   Coliforms
B.   Micrococcus lutes
C.   E. coli
D.   Bacillus subtilis
Question #8
What reagents are used in order to interpret the VP test?
A.   KOH-creatine only
B.   KOH-naphthol only
C.   Naphthol creatine only
D.   Alpha-naphthol only
E.   KOH-creatine & alpha-naphthol
F.   No additional reagents are needed.

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