Microbiology 020 - General Microbiology » Fall 2021 » Lab Quiz 5 Battery Tests
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Question #1
If bacteria in TSIA ferment sugars, _______ turns _______.
A.
Phenol red indicator; yellow
B.
Methyl red; red
C.
Phenol red indicator; red
D.
Methyl red; yellow
E.
Phenol red indicator; black
Question #2
Sulfur reduction in TSIA appears as _______.
A.
Black precipitate in the butt
B.
Yellow throughout
C.
Black precipitate in the slant
D.
Gas formation
Question #3
TSIA stands for ____.
A.
Total sugar iron agar
B.
Total sucrose inositol agar
C.
Triple sugar iron agar
Question #4
After inoculation and incubation, a SIM test appears very cloudy. This indicates _______.
A.
the test was done incorrectly.
B.
the bacterium produces tryptophanase.
C.
the bacterium is non-motile.
D.
more bacteria should have been inoculated into the test.
E.
the bacterium is motile.
Question #5
Is SIM selective, differential, both, or neither?
A.
Selective & differential
B.
None.
C.
Differential only
D.
Selective only
Question #6
How is the SIM test inoculated?
A.
Stab the medium in a straight line.
B.
Mix in 1 loopful of bacteria from an overnight broth culture.
C.
Spread the bacteria over the medium with a sterile swab.
D.
Stab the anaerobic region and streak the aerobic region.
Question #7
A lab scientist has conducted an IMVC test from bacteria isolated from a sick patient. The scientist is likely attempting to determine if the patient is infected by ______.
A.
Micrococcus lutes
B.
E. coli
C.
Coliforms
D.
Bacillus subtilis
Question #8
What reagents are used in order to interpret the VP test?
A.
KOH-creatine & alpha-naphthol
B.
Alpha-naphthol only
C.
KOH-creatine only
D.
KOH-naphthol only
E.
No additional reagents are needed.
F.
Naphthol creatine only
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