Microbiology 020 - General Microbiology » Fall 2021 » Lab Quiz 5 Battery Tests

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Question #1
If bacteria in TSIA ferment sugars, _______ turns _______.
A.   Phenol red indicator; black
B.   Methyl red; yellow
C.   Phenol red indicator; red
D.   Phenol red indicator; yellow
E.   Methyl red; red
Question #2
Sulfur reduction in TSIA appears as _______.
A.   Black precipitate in the slant
B.   Gas formation
C.   Black precipitate in the butt
D.   Yellow throughout
Question #3
TSIA stands for ____.
A.   Triple sugar iron agar
B.   Total sucrose inositol agar
C.   Total sugar iron agar
Question #4
After inoculation and incubation, a SIM test appears very cloudy. This indicates _______.
A.   the test was done incorrectly.
B.   the bacterium is motile.
C.   more bacteria should have been inoculated into the test.
D.   the bacterium is non-motile.
E.   the bacterium produces tryptophanase.
Question #5
Is SIM selective, differential, both, or neither?
A.   None.
B.   Selective & differential
C.   Selective only
D.   Differential only
Question #6
How is the SIM test inoculated?
A.   Mix in 1 loopful of bacteria from an overnight broth culture.
B.   Stab the anaerobic region and streak the aerobic region.
C.   Stab the medium in a straight line.
D.   Spread the bacteria over the medium with a sterile swab.
Question #7
A lab scientist has conducted an IMVC test from bacteria isolated from a sick patient. The scientist is likely attempting to determine if the patient is infected by ______.
A.   E. coli
B.   Coliforms
C.   Bacillus subtilis
D.   Micrococcus lutes
Question #8
What reagents are used in order to interpret the VP test?
A.   Alpha-naphthol only
B.   KOH-creatine & alpha-naphthol
C.   KOH-naphthol only
D.   No additional reagents are needed.
E.   Naphthol creatine only
F.   KOH-creatine only

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