Microbiology 020 - General Microbiology » Fall 2021 » Microbial Nutrition and Growth 1

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Question #1
Which descriptors are likely to have applied to the earliest microbes on the planet?
A.   Thermophilic only
B.   Chemoheterotrophic only
C.   Chemoautotrophic only
D.   Chemoautotrophic, Thermophilic, Chemoheterotrophic
Question #2
Match the correct essential nutrient to its best description based on your understanding of the roles of these nutrients : Phosphate
A.   This nutrient is a major part of all organic compounds in the cell as well as water, the major component of the cell
B.   A cell is unable to produce ATP and its cell membrane has lost its integrity because of a deficiency of this nutrient
C.   A cell is unable to synthesize proteins and nucleic acids due to a deficiency in this essential nutrient required for their structure
Question #3
Match the correct essential nutrient to its best description based on your understanding of the roles of these nutrients : Nitrogen
A.   A cell is unable to produce ATP and its cell membrane has lost its integrity because of a deficiency of this nutrient
B.   A fundamental component of organic molecules consumed by and produced by the cell
C.   A cell is unable to synthesize proteins and nucleic acids due to a deficiency in this essential nutrient required for their structure
Question #4
Match the correct essential nutrient to its best description based on your understanding of the roles of these nutrients : Sulfur
A.   This nutrient is a major part of all organic compounds in the cell as well as water, the major component of the cell
B.   A cell's proteins are misshapen or unstable because of its lack of the amino acids cysteine and methionine (composed in part by this nutrient)
C.   A fundamental component of organic molecules consumed by and produced by the cell
Question #5
Match the correct essential nutrient to its best description based on your understanding of the roles of these nutrients : Oxygen
A.   A cell requires this nutrient for pH maintenance and the bonds between DNA strands
B.   A fundamental component of organic molecules consumed by and produced by the cell
C.   This nutrient is a major part of all organic compounds in the cell as well as water, the major component of the cell
Question #6
Match the correct essential nutrient to its best description based on your understanding of the roles of these nutrients : Hydrogen
A.   A cell is unable to produce ATP and its cell membrane has lost its integrity because of a deficiency of this nutrient
B.   A cell requires this nutrient for pH maintenance and the bonds between DNA strands
C.   A fundamental component of organic molecules consumed by and produced by the cell
Question #7
Match the correct essential nutrient to its best description based on your understanding of the roles of these nutrients : Carbon
A.   A cell requires this nutrient for pH maintenance and the bonds between DNA strands
B.   A fundamental component of organic molecules consumed by and produced by the cell
C.   A cell is unable to produce ATP and its cell membrane has lost its integrity because of a deficiency of this nutrient
Question #8
Match the environment with the effects of a cell placed in that environment to test your understanding of the tonicity terms : Hypertonic environment
A.   A cell in this environment will lose water and shrink as water moves out of the cell by osmosis.
B.   A cell in this environment will swell as water moves in by osmosis, and may lyse if it does not have a cell wall.
C.   A cell in this environment will neither shrink nor swell as there is no net movement of water into or out of the cell by osmosis.
Question #9
Match the environment with the effects of a cell placed in that environment to test your understanding of the tonicity terms : Hypotonic environment
A.   A cell in this environment will swell as water moves in by osmosis, and may lyse if it does not have a cell wall.
B.   A cell in this environment will lose water and shrink as water moves out of the cell by osmosis.
C.   A cell in this environment will neither shrink nor swell as there is no net movement of water into or out of the cell by osmosis.
Question #10
Match the environment with the effects of a cell placed in that environment to test your understanding of the tonicity terms : Isotonic environment
A.   A cell in this environment will swell as water moves in by osmosis, and may lyse if it does not have a cell wall.
B.   A cell in this environment will neither shrink nor swell as there is no net movement of water into or out of the cell by osmosis.
C.   A cell in this environment will lose water and shrink as water moves out of the cell by osmosis.
Question #11
Match the term with the appropriate description to test your understanding of terminology regarding temperature preferences of microorganisms : psychrophile
A.   Describes an organism that has an optimum growth temperature between 15°C and 30°C, but is able to grow slowly at colder temperatures
B.   Describes an organism that grows optimally at temperatures ranging from 80°C to 120°C
C.   Describes an organism that grows optimally at temperatures ranging from 0°C to 15°C
Question #12
Match the term with the appropriate description to test your understanding of terminology regarding temperature preferences of microorganisms : psychrotolerant
A.   Describes an organism that grows optimally at temperatures ranging from 20°C to 40°C
B.   Describes an organism that has an optimum growth temperature between 15°C and 30°C, but is able to grow slowly at colder temperatures
C.   Describes an organism that grows optimally at temperatures ranging from 0°C to 15°C
Question #13
Match the term with the appropriate description to test your understanding of terminology regarding temperature preferences of microorganisms : mesophile
A.   Describes an organism that grows optimally at temperatures ranging from 0°C to 15°C
B.   Describes an organism that grows optimally at temperatures ranging from 20°C to 40°C
C.   Describes an organism that has an optimum growth temperature between 15°C and 30°C, but is able to grow slowly at colder temperatures
Question #14
Match the term with the appropriate description to test your understanding of terminology regarding temperature preferences of microorganisms : thermophile
A.   Describes an organism that grows optimally at temperatures ranging from 45°C to 80°C
B.   Describes an organism that grows optimally at temperatures ranging from 20°C to 40°C
C.   Describes an organism that grows optimally at temperatures ranging from 0°C to 15°C
Question #15
Match the term with the appropriate description to test your understanding of terminology regarding temperature preferences of microorganisms : Hyper––thermophile
A.   Describes an organism that grows optimally at temperatures ranging from 80°C to 120°C
B.   Describes an organism that grows optimally at temperatures ranging from 45°C to 80°C
C.   Describes an organism that grows optimally at temperatures ranging from 0°C to 15°C
Question #16
Match the term with the appropriate description to test your understanding of the diversity of oxygen requirements among microorganisms : Obligate aerobe
A.   Describes an organism that requires oxygen for growth and has the enzymes to remove toxic by-products of oxygen metabolism
B.   Describes an organism that is unable to use oxygen and lacks the enzymes to detoxify oxygen, thus requiring the absence of oxygen for growth
C.   Describes an organism that does not use oxygen, but can grow to a limited extent in the presence of oxygen
Question #17
Match the term with the appropriate description to test your understanding of the diversity of oxygen requirements among microorganisms : Facultative anaerobe
A.   Describes an organism that cannot grow at normal atmospheric oxygen concentrations, but does require a small amount of oxygen for growth
B.   Describes an organism that requires oxygen for growth and has the enzymes to remove toxic by-products of oxygen metabolism
C.   Describes an organism that can grow in the presence of oxygen and can detoxify it, but can also grow in the absence of oxygen
Question #18
Match the term with the appropriate description to test your understanding of the diversity of oxygen requirements among microorganisms : Microaerophile
A.   Describes an organism that cannot grow at normal atmospheric oxygen concentrations, but does require a small amount of oxygen for growth
B.   Describes an organism that can grow in the presence of oxygen and can detoxify it, but can also grow in the absence of oxygen
C.   Describes an organism that requires oxygen for growth and has the enzymes to remove toxic by-products of oxygen metabolism
Question #19
Match the term with the appropriate description to test your understanding of the diversity of oxygen requirements among microorganisms : Obligate anaerobe
A.   Describes an organism that is unable to use oxygen and lacks the enzymes to detoxify oxygen, thus requiring the absence of oxygen for growth
B.   Describes an organism that cannot grow at normal atmospheric oxygen concentrations, but does require a small amount of oxygen for growth
C.   Describes an organism that requires oxygen for growth and has the enzymes to remove toxic by-products of oxygen metabolism
Question #20
Match the term with the appropriate description to test your understanding of the diversity of oxygen requirements among microorganisms : Aerotolerant anaerobe
A.   Describes an organism that does not use oxygen, but can grow to a limited extent in the presence of oxygen
B.   Describes an organism that is unable to use oxygen and lacks the enzymes to detoxify oxygen, thus requiring the absence of oxygen for growth
C.   Describes an organism that requires oxygen for growth and has the enzymes to remove toxic by-products of oxygen metabolism
Question #21
Match the phase of the bacterial growth curve with its correct description : Stationary growth phase
A.   Adjustment period during which cells are dividing slowly while they adjust to growth conditions
B.   Phase during which cells are growing very quickly, at their maximum rate of cell division
C.   Phase during which nutrient depletion and accumulation of wastes begin to slow cell growth such that the rate of cell increase equals the rate of cell death
Question #22
Match the phase of the bacterial growth curve with its correct description : Exponential growth phase
A.   Adjustment period during which cells are dividing slowly while they adjust to growth conditions
B.   Phase during which nutrient depletion and accumulation of wastes begin to slow cell growth such that the rate of cell increase equals the rate of cell death
C.   Phase during which cells are growing very quickly, at their maximum rate of cell division
Question #23
Match the phase of the bacterial growth curve with its correct description : Lag phase
A.   Nutrient depletion and waste accumulation prevent cell growth and cells die exponentially
B.   Adjustment period during which cells are dividing slowly while they adjust to growth conditions
C.   Phase during which cells are growing very quickly, at their maximum rate of cell division
Question #24
Match the phase of the bacterial growth curve with its correct description : Death phase
A.   Adjustment period during which cells are dividing slowly while they adjust to growth conditions
B.   Phase during which cells are growing very quickly, at their maximum rate of cell division
C.   Nutrient depletion and waste accumulation prevent cell growth and cells die exponentially
Question #25
Match the method of counting bacteria with the correct descriptive statement : Turbidometry
A.   This method uses a special slide (cytometer) and cells are counted by observing them through a microscope
B.   An automated cell-counting device that requires fluorescent labeling of cells that will be scanned and counted
C.   A spectrophotometer is used to measure the light scattering properties of a bacterial culture. As the number of bacteria increase, more light is scattered and less is transmitted to the detector
Question #26
Match the method of counting bacteria with the correct descriptive statement : Direct cell count
A.   This method uses a special slide (cytometer) and cells are counted by observing them through a microscope
B.   This method uses a special slide (cytometer) and cells are counted by observing them through a microscope
C.   A spectrophotometer is used to measure the light scattering properties of a bacterial culture. As the number of bacteria increase, more light is scattered and less is transmitted to the detector
Question #27
Match the method of counting bacteria with the correct descriptive statement : Coulter counter
A.   An automated cell-counting device that scans a culture as it passes through a tiny pipette
B.   A spectrophotometer is used to measure the light scattering properties of a bacterial culture. As the number of bacteria increase, more light is scattered and less is transmitted to the detector
C.   This method uses a special slide (cytometer) and cells are counted by observing them through a microscope
Question #28
Match the method of counting bacteria with the correct descriptive statement : Flow cytometry
A.   An automated cell-counting device that scans a culture as it passes through a tiny pipette
B.   An automated cell-counting device that requires fluorescent labeling of cells that will be scanned and counted
C.   An automated cell-counting device that scans a culture as it passes through a tiny pipette
Question #29
Both human and bacterial cells divide by mitosis.
A.   FALSE
B.   TRUE
Question #30
Cells must ________ their DNA prior to cell division.
A.   transcribe
B.   hydrolyze
C.   translate
D.   replicate
Question #31
Environmental factors control microbial growth through their influence on enzyme activity.
A.   TRUE
B.   FALSE
Question #32
The FtsZ protein is needed to form the ________.
A.   ribosomes
B.   All of the choices are correct.
C.   septum
D.   replicated DNA
Question #33
The energy source for contraction of the FtsZ ring during cell division comes from ________.
A.   the hydrolysis of GTP
B.   the synthesis of G
C.   the hydrolysis of gyrase
D.   the hydrolysis of ATP
Question #34
Which enzyme is NOT involved in DNA replication?
A.   Helicase
B.   RNA polymerase
C.   Gyrase
D.   DNA polymerase
Question #35
Cells cannot begin a new round of replication until they have completely finished dividing.
A.   TRUE
B.   FALSE
Question #36
Most cultured bacteria tend to multiply by ________.
A.   fragmentation
B.   sexual reproduction
C.   binary fission
D.   budding
Question #37
Cell division is triggered exclusively when cells attain a threshold mass.
A.   FALSE
B.   TRUE
Question #38
DNA helicase is responsible for adding nucleotides to the growing end of the DNA.
A.   TRUE
B.   FALSE
Question #39
In the United States, the CDC estimates that ________ people suffer each year from some form of foodborne infection.
A.   roughly 100,000
B.   between 1.5 and 2 billion
C.   several million
D.   several thousand
Question #40
The presence of microbes in food can be ________.
A.   neutral
B.   harmful
C.   beneficia
D.   All of the choices are correct.
Question #41
Individual bacteria (such as Salmonella) ________.
A.   can grow at a broad range of temperatures from -5°C to 80°C
B.   grow at different temperatures based on the availability of oxygen
C.   grow best at an optimum temperature and a range above and below it
D.   grow at different temperatures based on the availability of nutrients
Question #42
The type of food-borne infection known as an intoxication is caused by ________.
A.   growth of the microbe in the intestinal tract and intestinal lining
B.   the buildup of oxidative by-products such as O- in the food
C.   toxins produced by the microbe in the intestinal tract
D.   toxins produced by microbes as they grow in the food
Question #43
  
A.   budding
B.   endospores
C.   sexual reproduction
D.   binary fission
Question #44
After six generations, how many bacteria would have formed from the reproduction of one bacterium?
A.   64
B.   12
C.   6
D.   32
Question #45
Bacteria that grow in oxygenated environments are referred to as ________.
A.   anaerobes
B.   aerobes
C.   capnophiles
D.   methanogens
Question #46
Bacteria that thrive in environments with a pH of greater than 7 are classified as ________.
A.   alkalinophiles
B.   acidophiles
C.   neutrophiles
Question #47
What is the effect of heat on a population of bacteria?
A.   Cells begin to die at a temperature slightly above the maximum.
B.   Cells survive but do not divide at temperatures above the maximum.
C.   Cells divide more rapidly as the temperature increases above the optimum.
D.   Cells gradually die as temperatures rise above the minimum.
Question #48
What is the effect of cold on a population of bacteria?
A.   Cells divide more rapidly as the temperature decreases below the optimum.
B.   Cells survive but do not divide at temperatures below the minimum.
C.   Cells begin to rapidly die at a temperature just below the optimum.
Question #49
What food product is least likely to contain viable vegetative pathogens?
A.   Chicken cooked to 170°F
B.   Food stored at 60°F
C.   Food stored in a refrigerator
D.   Food frozen to 0°C
Question #50
If conditions are favorable, the generation time of bacteria _______.
A.   can be as short as 12 hours
B.   increase over that seen in unfavourable conditions
C.   is remarkably stable, showing little change
D.   can be as short as 30 minutes
Question #51
The environmental factor that influences bacterial growth the most is _______.
A.   nutrients
B.   pH
C.   temperature
D.   oxygen
Question #52
  
A.   the low temperature where they physically die and the high temperature where they stop multiplying
B.   the low temperature where microbes stop multiplying and the high temperature where they stop multiplying
C.   the low temperature where microbes physically die and the high temperature where they physically die
D.   the low temperature where microbes stop multiplying and the high temperature where they physically die

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