The process of passing a mobile phase through a chromatography column is called which one of the following?

The pH at which a protein carries a net zero charge is termed which of the following?

A characteristic feature of any form of chromatography is the …

The molecular weight of an unspecified protein, at physiological conditions, is 70,000 Dalton. The SDS-polyacrylamide gel electrophoresis (SDS PAGE) of the protein yields a single band corresponding to molecular weight of 70,000 Dalton. However, in the presence of the reducing agent, β-mercaptoethanol, the SDS PAGE shows two bands, corresponding to molecular weights of 30,000 and 20,000 Dalton. From these data, describe the native protein in terms of the number of subunits present, their molecular weight, stoichiometry of subunits, and the kinds of bonding existing between the subunits.

The basis of the technique of chromatography for separating components of a mixture is

In a crude extract, globular proteins are usually NOT dissolved in the buffer used for extraction and can be collected by centrifugation.

What is the purpose of the gel in SDS-PAGE?

Which of the following amino acids would be first to elute at pH 7 from an anion-exchange column?

Sodium dodecyl sulfate (SDS) gel electrophoresis is often used for

Salting out process involves

A distracted student lets a SDS PAGE gel run for too long. When the student realizes her mistake, it is too late and only four out of the five bands that she is supposed to see on the gel appear. Out of the five proteins listed below, which one has probably run out of the gel?

When separating proteins using gel filtration column chromatography, the first proteins to be eluted will be ________________.

Separation of proteins by gel filtration column chromatography take advantage of differences in

Dialysis can be used instead of gel filtration chromatography as a desalting device.