Chem 221 - Biochemistry for Science Majors » Fall 2021 » Working with Proteins

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Question #1
What type of sample does scientist in the video start with?
A.   Frozen heart
B.   Cell pellet
C.   Chicken breast
D.   Crude extract
Question #2
What is the FIRST method used in the video to create the crude extract?
A.   Blender
B.   Homogenization
C.   Sonication
Question #3
What is the second method used in the video to create the crude extract?
A.   Sonication
B.   Homogenization
C.   Blender
Question #4
How does the scientist prevent denaturation during protein extraction?
A.   Mixes the sample multiple times
B.   Keeps sample on ice
C.   Boils the sample
D.   Works really fast
Question #5
What is the speed used to centrifuge the balanced tubes?
A.   Lowest speed
B.   Highest speed
C.   Medium speed
Question #6
What is the brand of the centrifuge rotor?
A.   Smith
B.   Biorad
C.   Sorvall
Question #7
Centrifugation time is
A.   90 minutes
B.   40 minutes
C.   20 minutes
D.   60 minutes
Question #8
After centrifugation, supernatant is filtered.
A.   FALSE
B.   TRUE
Question #9
Look up the meaning of FPLC (we did not discussed it in class). The most common FPLC is
A.   Ion exchange
B.   Affinity chromatography
Question #10
To load sample in the SDS PAGE apparatus
A.   A micropipette is used
B.   A Mohr pipette is used
Question #11
The color of the sample loaded in the SDS PAGE wells is
A.   Blue
B.   Pink
C.   Green
Question #12
Observe the gel. Do the bands show proteins with about the same weight or different weights?
A.   Same
B.   Different

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