Microbiology 020 - General Microbiology » Fall 2021 » Acid Fast Staining Lab

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Question #1
Bacteria that have a waxy lipid cell wall made of _____ acid are called acid-fast bacteria.
A.   hydrophilic
B.   mycolic
Question #2
This waxy lipid cell wall prevents the entry of _____ material such as antibiotic drugs and disinfectants.
A.   hydrophilic
B.   mycolic
Question #3
Two common genera of bacteria that are acid-fast: _____ and _____.
A.   Mycobacteria and Nocardia
B.   mycolic and hydrophilic
Question #4
The acid-fast staining technique is called the _______ method.
A.   Ziehl-Nielsen
B.   cording
Question #5
The primary stain in this technique is _____ which stains the acid-fast bacteria to be ______. This stain requires ____ heat to allow it absorbed by the cell wall more readily.
A.   carbolfuchsin…red-colored…steam…
B.   nocardia…..blue colored… steam
Question #6
The counterstain in this technique is ______ which stains the non-acid-fast bacteria to be ______.
A.   methylene red….red-colored
B.   methylene blue…...blue-colored
Question #7
Acid-fast bacteria tend to clump together due their sticky waxy-lipid cell wall, a phenomenon called _____.
A.   Ziehl-Nielsen
B.   cording

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