Microbiology 020 - General Microbiology » Fall 2021 » Capsular Staining Lab

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Question #1
Capsules are made of ______ and _____.
A.   polysaccharides and proteins
B.   sucrose… lipids…
Question #2
Name two functions of capsules.
A.   promotes dessication and promotes phagocytosis
B.   prevents dessication and prevents phagocytosis
Question #3
Name two pathogens that have capsules.
A.   Mycobacteria and Nocardia
B.   Streptococcus pneumoniae and Escherichia coli
Question #4
Because capsules have a high water content and cannot be stained, this technique will stain the background instead, a method called ___ staining. This technique also deletes the step of ___ fixing after air-drying because it could distort the shape of the capsules.
A.   acid.… moisture…
B.   capsule… heat…
Question #5
The first dye in this technique is _____ which is ___ (acidic/basic), and it stains the background to be ____.
A.   Congo Red I solution… acidic… red-colored.
B.   Maneval's stain … basic… blue -colored.
Question #6
The second dye in this technique is _____ which is ___ , and it will change the background to be _____.
A.   Maneval's stain … basic… blue -colored.
B.   Congo Red I solution… acidic… red-colored.
Question #7
The final appearance of the slide will be a _________ background, bacteria stained to be _____, and the capsules are ___.
A.   grayish blue-colored … purple_-colored… white.
B.   grayish blue-colored … red-colored… blue.

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