Microbiology 020 - General Microbiology » Fall 2021 » Capsular Staining Lab
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Question #1
Capsules are made of ______ and _____.
A.
polysaccharides and proteins
B.
sucrose… lipids…
Question #2
Name two functions of capsules.
A.
promotes dessication and promotes phagocytosis
B.
prevents dessication and prevents phagocytosis
Question #3
Name two pathogens that have capsules.
A.
Mycobacteria and Nocardia
B.
Streptococcus pneumoniae and Escherichia coli
Question #4
Because capsules have a high water content and cannot be stained, this technique will stain the background instead, a method called ___ staining. This technique also deletes the step of ___ fixing after air-drying because it could distort the shape of the capsules.
A.
acid.… moisture…
B.
capsule… heat…
Question #5
The first dye in this technique is _____ which is ___ (acidic/basic), and it stains the background to be ____.
A.
Congo Red I solution… acidic… red-colored.
B.
Maneval's stain … basic… blue -colored.
Question #6
The second dye in this technique is _____ which is ___ , and it will change the background to be _____.
A.
Maneval's stain … basic… blue -colored.
B.
Congo Red I solution… acidic… red-colored.
Question #7
The final appearance of the slide will be a _________ background, bacteria stained to be _____, and the capsules are ___.
A.
grayish blue-colored … purple_-colored… white.
B.
grayish blue-colored … red-colored… blue.
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