Microbiology 020 - General Microbiology » Fall 2021 » Pure Culture Techniques Lab
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Question #1
Always sterilize the inoculating loop at the ___ of the blue cone in the flame.
A.
bottom
B.
tip
Question #2
The technique begins with streaking of the culture at the upper left corner of the agar plate, about ___ times.
A.
ten
B.
five
Question #3
In subsequent streaks, always turn the agar plate about ___degrees and streak the culture to a new area.
A.
90
B.
45
Question #4
At the end, there should be ___ areas of streaks on the plate. Area I should have the ___ amount of bacteria, while Area IV should have the ___ amount of bacteria.
A.
four…most…least…
B.
three…...least… most
Question #5
This technique requires 3 test tubes of ____ agar and 3 ____ petri plates.
A.
empty… molten…
B.
molten…empty…
Question #6
After transferring bacteria from the culture to tube I, we should ____ the test tube to mix the bacteria with the agar evenly.
A.
stir
B.
roll
Question #7
After the agar from the 3 test tubes is poured into the petri plates, plate I should have the _____ amount of bacteria, while plate III should have the _____ amount.
A.
most diluted… most concentrated…
B.
most concentrated… most diluted…
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