Bio 2010 - Microbiology » Fall 2021 » Aseptic Transfer Technique (2)

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Question #1
Which of the following best describes contamination?
A.   presence of oxygen
B.   presence of microorganisms
C.   more than one of the above is correct
D.   oxygen free
E.   presence of carbon dioxide
F.   none of the above is correct
Question #2
How should you sterilize materials that will make contact with your culture media?
A.   wash them in the sink
B.   by using the Bunsen burner and flaming them
C.   more than one of the above is correct
D.   wiping it down with a tissue
E.   you don't need to sterilize materials that will make contact with your experiment
F.   none of the above is correct
Question #3
Where should Petri dish lids be placed during the aseptic transfer technique?
A.   in your hand, facing down
B.   in your hand, facing up
C.   more than one of the above is correct
D.   on the table, facing up
E.   on the table, facing down
F.   none of the above is correct
Question #4
Once inoculated with bacteria, how should agar Petri plates be incubated?
A.   sideways
B.   right side up
C.   more than one of the above is correct
D.   upside down
E.   none of the above is correct
Question #5
Why is it important to flame the test tubes before and after transferring bacteria into fresh, sterile culture media?
A.   to seal the plastic lid on the tube
B.   more than one of the above is correct
C.   prevent contaminating the second culture tube
D.   clean the tube for use in the future
E.   none of the above is correct

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