Bio 2010 - Microbiology » Fall 2021 » Aseptic Transfer Technique (2)

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Question #1
Which of the following best describes contamination?
A.   presence of oxygen
B.   more than one of the above is correct
C.   oxygen free
D.   presence of carbon dioxide
E.   presence of microorganisms
F.   none of the above is correct
Question #2
How should you sterilize materials that will make contact with your culture media?
A.   by using the Bunsen burner and flaming them
B.   wiping it down with a tissue
C.   you don't need to sterilize materials that will make contact with your experiment
D.   more than one of the above is correct
E.   wash them in the sink
F.   none of the above is correct
Question #3
Where should Petri dish lids be placed during the aseptic transfer technique?
A.   on the table, facing up
B.   in your hand, facing down
C.   in your hand, facing up
D.   on the table, facing down
E.   more than one of the above is correct
F.   none of the above is correct
Question #4
Once inoculated with bacteria, how should agar Petri plates be incubated?
A.   upside down
B.   right side up
C.   sideways
D.   more than one of the above is correct
E.   none of the above is correct
Question #5
Why is it important to flame the test tubes before and after transferring bacteria into fresh, sterile culture media?
A.   more than one of the above is correct
B.   prevent contaminating the second culture tube
C.   to seal the plastic lid on the tube
D.   clean the tube for use in the future
E.   none of the above is correct

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