Bio 2010 - Microbiology » Fall 2021 » Aseptic Transfer Technique (2)

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Question #1
Which of the following best describes contamination?
A.   oxygen free
B.   more than one of the above is correct
C.   presence of oxygen
D.   presence of microorganisms
E.   presence of carbon dioxide
F.   none of the above is correct
Question #2
How should you sterilize materials that will make contact with your culture media?
A.   more than one of the above is correct
B.   you don't need to sterilize materials that will make contact with your experiment
C.   wiping it down with a tissue
D.   wash them in the sink
E.   by using the Bunsen burner and flaming them
F.   none of the above is correct
Question #3
Where should Petri dish lids be placed during the aseptic transfer technique?
A.   more than one of the above is correct
B.   in your hand, facing up
C.   on the table, facing up
D.   on the table, facing down
E.   in your hand, facing down
F.   none of the above is correct
Question #4
Once inoculated with bacteria, how should agar Petri plates be incubated?
A.   upside down
B.   more than one of the above is correct
C.   right side up
D.   sideways
E.   none of the above is correct
Question #5
Why is it important to flame the test tubes before and after transferring bacteria into fresh, sterile culture media?
A.   prevent contaminating the second culture tube
B.   more than one of the above is correct
C.   clean the tube for use in the future
D.   to seal the plastic lid on the tube
E.   none of the above is correct

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