Bio 2010 - Microbiology » Fall 2021 » Aseptic Transfer Technique (2)

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Question #1
Which of the following best describes contamination?
A.   presence of oxygen
B.   presence of carbon dioxide
C.   oxygen free
D.   presence of microorganisms
E.   more than one of the above is correct
F.   none of the above is correct
Question #2
How should you sterilize materials that will make contact with your culture media?
A.   wash them in the sink
B.   wiping it down with a tissue
C.   by using the Bunsen burner and flaming them
D.   you don't need to sterilize materials that will make contact with your experiment
E.   more than one of the above is correct
F.   none of the above is correct
Question #3
Where should Petri dish lids be placed during the aseptic transfer technique?
A.   in your hand, facing down
B.   in your hand, facing up
C.   more than one of the above is correct
D.   on the table, facing up
E.   on the table, facing down
F.   none of the above is correct
Question #4
Once inoculated with bacteria, how should agar Petri plates be incubated?
A.   right side up
B.   upside down
C.   more than one of the above is correct
D.   sideways
E.   none of the above is correct
Question #5
Why is it important to flame the test tubes before and after transferring bacteria into fresh, sterile culture media?
A.   clean the tube for use in the future
B.   more than one of the above is correct
C.   to seal the plastic lid on the tube
D.   prevent contaminating the second culture tube
E.   none of the above is correct

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