Bio 2010 - Microbiology » Fall 2021 » Lab Quiz 1 (2)

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Question #1
Why is it important to flame the test tubes before and after transferring bacteria into fresh, sterile culture media?
A.   clean the tube for use in the future
B.   more than one of the above is correct
C.   prevent contaminating the second culture tube
D.   to seal the plastic lid on the tube
E.   none of the above is correct
Question #2
Choose the term below, that BEST completes the following statement. As magnification increases, the contrast:
A.   increases
B.   decreases
C.   stays the same
Question #3
After making and incubating your Streak-for-Isolation plate a student notices that they have confluent growth in EVERY sector/quadrant. Which of the following most likely explains what happened?
A.   the tryptic soy agar plate was contaminated prior to making the streak
B.   they left the top of the Petri open too long during making the streak
C.   the bacteria spread through each sector during incubation
D.   they returned the flame loop to the broth between every sector/quadrant
Question #4
Which of the following choices is the BEST definition of depth-of-field?
A.   the image that one sees when looking through the oculars
B.   the distance between the end of the microscope objective lens and the specimen slide
C.   the ability to focus above or below a stained specimen
D.   the ability to distinguish two small objects as separate

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