Microbiology 020 - General Microbiology » Winter 2022 » Lab Quiz 5-Battery Tests

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Question #1
Which of the following reagents must be added to the TSIA tube after incubation?
A.   None.
B.   Alpha-naphthol
C.   Kovac's reagent
D.   KOH-creatine
Question #2
To inoculate TSIA, one should ______.
A.   Stab the anaerobic region and smear the aerobic region.
B.   Stab the aerobic region.
C.   Pour in 1 mL of bacteria from an overnight broth culture.
D.   Smear the aerobic region.
Question #3
Black precipitate will only occur when ______.
A.   the bacteria are non-motile.
B.   there is no change in the medium.
C.   the medium is alkaline.
D.   the medium is acidic.
Question #4
Is TSIA selective, differential, both, or neither?
A.   Both selective & differential
B.   Differential only
C.   None.
D.   Selective only
Question #5
After adding the appropriate reagent to a SIM tube after incubation, a bright pink layer forms at the top. This means the bacteria produce _____.
A.   Tryptophan
B.   Catalase
C.   Tryptophanase
D.   Amino acids
Question #6
The SIM test is useful for differentiating _______.
A.   S. pyogenes
B.   Neisseria species
C.   Streptococci from staphylococci
D.   Enterobacteriaceae
Question #7
What does SIM stand for?
A.   Sugar Inositol Medium
B.   The correct answer is not listed here.
C.   Sucrose Inositol Medium
D.   Sulfur Indole Motility
Question #8
The IMVC test is useful for differentiating ______.
A.   Streptococci
B.   E. coli
C.   Fungi
D.   Enterobacteriaceae
E.   Staphylococci
Question #9
If the bacteria do mixed acid-fermentation, the methyl red test should appear ___.
A.   Yellow with black precipitate
B.   Red
C.   The correct answer is not listed here.
D.   Yellow
E.   Orange
Question #10
What reagents are used in order to interpret the VP test?
A.   KOH-creatine only
B.   KOH-naphthol only
C.   Naphthol creatine only
D.   No additional reagents are needed.
E.   Alpha-naphthol only
F.   KOH-creatine & alpha-naphthol

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