Microbiology 020 - General Microbiology » Winter 2022 » Lecture Exam 2 (Ch 5, 6, 7, 8)

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Question #1
Which of the following will not support viral cultivation?
A.   Live lab animals
B.   Continuous cell cultures
C.   Primary cell cultures
D.   All of thechoices will support viral cultivation.
E.   Embryonated bird eggs
Question #2
Persistent viruses that can reactivate periodically are _____.
A.   syncytia
B.   chronic latent viruses
C.   oncoviruses
D.   inclusion bodies
E.   cytopathic
Question #3
The envelope of enveloped viruses________.
A.   is obtained by viral budding or exocytosis
B.   does not contain spikes
C.   None of the choices are correct
D.   is only composed of host endomembrane
E.   is identical to the host plasma membrane
Question #4
Select the three choices that are the primary purposes of cultivating viruses.
A.   To produce virusfor bioweapons use To produce chemicals such as alcohol and acetone To isolate viruses from clinical specimens
B.   To research the biology of viruses To produce virusfor bioweapons use To produce chemicals such as alcohol and acetone
C.   To produce virusfor bioweapons use To prepare viruses for vaccines To produce chemicals such as alcohol and acetone
D.   To isolate viruses from clinical specimens To research the biology of viruses To prepare viruses for vaccines
Question #5
During lysogeny, an inactive prophage state occurs when the viral DNA is inserted into the ________.
A.   host nucleolus
B.   host DNA
C.   host cytoplasm
D.   host cell membrane
E.   host nucleus
Question #6
Select the three methods that are used to cultivate viruses.
A.   Use of enriched broth media Use of cellculture techniques Use of enrichedagar media
B.   Inoculation ofembryonated eggs, Use of animalinoculation, Use of cellculture techniques
C.   Use of animalinoculation Use of enriched broth media Use of enrichedagar media
D.   Use of enriched broth media, Use of enrichedagar media, Inoculation ofembryonated eggs
Question #7
A(n) _______ is the protein shell around the nucleic acid core of a virus.
A.   spike
B.   monolayer
C.   capsomere
D.   envelope
E.   capsid
Question #8
The process of dissolving the envelope and capsid to release the viral nucleic acid is _______.
A.   synthesis
B.   adsorption
C.   assembly
D.   penetration
E.   uncoating
Question #9
Which of the following is not a typical capsid shape?
A.   Icosahedron
B.   Helical
C.   Tetrahedral
D.   Complex
E.   All of thechoices are capsid shapes.
Question #10
A negative-sense RNA virus ________.
A.   must synthesize a positive RNA copy of its genome
B.   is ready for immediate translation
C.   always codes for RNA polymerase
D.   must synthesize a negative RNA copy of its genome
E.   is a special form of tRNA used by viruses
Question #11
Viruses that cause infection resulting in alternating periods of activity with symptoms and inactivity without symptoms are called _____.
A.   viroids
B.   latent
C.   prions
D.   oncogenic
E.   delta agents
Question #12
Viral tissue specificities are called _____.
A.   uncoating
B.   virions
C.   tropisms
D.   receptacles
E.   ranges
Question #13
Viruses that infect bacteria are specifically called _______.
A.   All of thechoices infect bacteria.
B.   prions
C.   bacteriophages
D.   viroids
E.   satellite viruses
Question #14
Infectious protein particles are called _______.
A.   prions
B.   phages
C.   oncogenic viruses
D.   spikes
E.   viroids
Question #15
Which of the following is incorrect about prophages?
A.   Replicated with host DNA and passed on to progeny
B.   Formed when viral DNA enters the bacterial chromosome
C.   Occur when temperate phages enter host cells
D.   Present when the virus is in lysogeny
E.   Cause lysis of host cells
Question #16
Host range is limited by________.
A.   age of the host cell
B.   All of the choices are correct
C.   type of host cell receptors on cell membrane
D.   type of nucleic acid in the virus
E.   size of the host cell
Question #17
Mammalian viruses capable of starting tumors are _____.
A.   syncytia
B.   inclusion bodies
C.   oncoviruses
D.   chronic latent viruses
E.   cytopathic
Question #18
Viral nucleic acids include which of the following?
A.   All of the choices are correct
B.   Single-stranded RNA
C.   Double-stranded DNA
D.   Double-stranded RNA
E.   Single-stranded DNA
Question #19
Lysogeny refers to ________.
A.   None of the choices are correct
B.   latent state of herpes infections
C.   viral genome inserting into bacterial host chromosome
D.   virion exiting host cell
E.   altering the host range of a virus
Question #20
When whole cells or large molecules in solution are engulfed by a cell, this endocytosis is specifically termed _______.
A.   pinocytosis
B.   facilitated transport
C.   facilitated diffusion
D.   exocytosis
E.   phagocytosis
Question #21
What type of media is used to demonstrate oxygen requirements of microbes?
A.   Sulfite polymyxin sulfadiazine
B.   None of the choices are correct.
C.   Sodium chloride
D.   Blood agar
E.   Thioglycollate
Question #22
Which of the following is not a major element of a microbial cell?
A.   Nitrogen
B.   Hydrogen
C.   Carbon
D.   Copper
E.   Oxygen
Question #23
Nutrient absorption is mediated by the________.
A.   nuclear membrane
B.   cell membrane
C.   proteins in the periplasmic space
D.   cell wall
E.   peptidoglycan layer
Question #24
An important mineral ion that is a component of chloroplasts and stabilizer of membranes and ribosomes is _______.
A.   zinc
B.   iron
C.   magnesium
D.   potassium
E.   calcium
Question #25
Select all of the cellular transport mechanisms that require energy.
A.   Simple diffusion and Bulk transport (phagocytosis, pinocytosis)
B.   Facilitated diffusion and Simple diffusion
C.   Facilitated diffusion and Carrier-mediated active transport
D.   Carrier-mediated active transport and Bulk transport (phagocytosis, pinocytosis)
Question #26
Facilitated diffusion is limited by________.
A.   osmotic pressure
B.   substrate concentration
C.   size of the pores in the membrane
D.   the size of the cell
E.   carrier proteins in the membrane
Question #27
Human pathogens fall into the group termed _______.
A.   halophiles
B.   psychrophiles
C.   thermophiles
D.   mesophiles
E.   acidophiles
Question #28
The movement of molecules from an area of high concentration to an area of lower concentration is called _______.
A.   diffusion
B.   facilitated diffusion
C.   active transport
D.   endocytosis
E.   osmosis
Question #29
The toxic superoxide ion is converted to harmless oxygen by two enzymes, ________.
A.   catalase and oxidase
B.   superoxide dismutase and hydrogen peroxidases
C.   superoxide dismutase and oxidase
D.   superoxide dismutase and catalase
E.   catalase and hydrogen peroxidases
Question #30
The term obligate refers to________.
A.   existing in a very narrow niche
B.   the ability to exist in a wide range of conditions
C.   using chemicals for energy production
D.   using oxygen for metabolism
E.   using light for energy production
Question #31
What compound is the most abundant in a cell?
A.   NH 3
B.   CO 2
C.   H 2O
D.   Glucose
E.   CH 4
Question #32
All of the following could find a location in or on body tissues suitable for growth except _______.
A.   psychrophiles
B.   mesophiles
C.   capnophiles
D.   anaerobes
E.   facultative anaerobes
Question #33
Bacterial biofilms stimulate the immune system differently than free (planktonic) bacteria.
A.   False
B.   True
Question #34
Aerobic respiration is an example of _______.
A.   photoautotrophy
B.   photosynthesis
C.   photoheterotrophy
D.   methanoheterophy
E.   chemoheterotrophy
Question #35
Most of the dry weight of a microbial cell is from _______.
A.   minerals
B.   water
C.   salts
D.   inorganic compounds
E.   organic compounds
Question #36
An organism that cannot tolerate an oxygen environment is a(n) _______.
A.   microaerophile
B.   obligate anaerobe
C.   facultative anaerobe
D.   aerotolerant anaerobe
E.   obligate aerobe
Question #37
Each of the following are denaturing agents except ________.
A.   low pH
B.   low temperature
C.   high pH
D.   All of the choices are correct.
E.   high temperature
Question #38
FAD, NAD, and coenzyme A are all _______ carriers.
A.   hydrogen andelectron
B.   ATP
C.   hydrogen
D.   electron
E.   None of the choices are correct.
Question #39
Exergonic reactions ________.
A.   include synthesis of large carbohydrates
B.   occur when ADP binds to inorganic phosphate to form ATP
C.   do not occur in anaerobic cellular respiration
D.   release energy
E.   only occur in heterotrophs
Question #40
The cell's metabolic reactions involve the participation of ________ that lower the activation energy needed for the initiation of a reaction.
A.   ATP
B.   enzymes
C.   coenzymes
D.   vitamins
E.   cofactors
Question #41
In a bacterial cell, the genes that code for enzymes that catalyze the hydrolysis of an unusual carbohydrate source would be turned off unless that carbohydrate was the only fuel source available in the media, in which case________.
A.   the enzymes would be denatured
B.   the genes coding for the hydrolytic enzymes would be constitutive
C.   the genes coding for the hydrolytic enzymes would be induced
D.   the genes coding for the hydrolytic enzymes would be repressed
Question #42
How many carbon dioxide molecules are produced by the complete aerobic breakdown of one glucose molecule?
A.   5
B.   6
C.   3
D.   4
E.   2
Question #43
Which of the following is not true of anaerobic respiration?
A.   Involves glycolysis
B.   Utilizes an electron transport system
C.   Generates some ATP
D.   Uses the same final electron acceptor as aerobic respiration
Question #44
Which of the following is not involved in the step that occurs between glycolysis and the Krebs cycle?
A.   CO2 is released
B.   Pyruvic acid accepts electrons from NADH
C.   NADH is formed
D.   Coenzyme A attaches to an acetyl group
Question #45
In bacterial cells, when glucose is completely oxidized by all the pathways of aerobic cellular respiration, what is the maximum number of ATP generated?
A.   38 ATP
B.   24 ATP
C.   42 ATP
D.   2 ATP
E.   3 ATP
Question #46
Enzymes that hydrolyze a peptide bond are called _______.
A.   phosphotransferases
B.   oxidoreductases
C.   peptidases
D.   ligases
E.   aminotransferases
Question #47
When glucose is broken down by glycolysis during bacterial fermentation, how many ATP are generated?
A.   2 ATP
B.   24 ATP
C.   3 ATP
D.   38 ATP
E.   36 ATP
Question #48
Each of the following is true of enzymes except ________.
A.   they can be used over and over
B.   All of the choices are true of enzymes.
C.   they increase the initial energy required for a reaction to start
D.   their active site is specific to the substrate
E.   they may or may not require cofactors
Question #49
The property of organisms to integrate catabolic and anabolic pathways to improve cell efficiency is called _______.
A.   metabolism
B.   anabolism
C.   amphibolism
D.   catabolism
E.   biosynthesis
Question #50
Fatty acids can be converted to acetyl-CoA for entering the Krebs cycle by the process of _______.
A.   amination
B.   gluconeogenesis
C.   deamination
D.   beta oxidation
E.     
F.   phosphorylation
Question #51
Reduction of nitrogen-oxygen ions, such as nitrate, by some bacteria is called _______.
A.   aerobic respiration
B.   denitrification
C.   deamination
D.   fermentation
E.   nitrification
Question #52
During which phase of cellular respiration is the majority of ATP formed?
A.   Glycolysis
B.   Electron transport
C.   All phasesproduce the same number of ATP molecules.
D.   Krebs cycle
E.   Processing of pyruvic acid for the Krebs cycle
Question #53
In addition to electrons, which of the following can also be transferred, exchanging energy in the process?
A.   Hydrogen ion (proton)
B.   Carbon dioxide
C.   Carbon
D.   Glucose
E.   ADP
Question #54
The _______ strand is synthesized continuously while the ________ strand is synthesized in a series of short fragments called Okazaki fragments.
A.   original; primary
B.   primary; secondary
C.   lagging; leading
D.   leading; lagging
Question #55
Mastery of synthetic biology will allow humans to ________.
A.   construct complex molecules in situ that will target cancerous cells in tissues
B.   design alternative forms of energy production
C.   All of the choices are possibilities from synthetic biology
D.   synthesize chemicals in cells that are missing due to a genetic anomaly
E.   make new organisms using only chemicals as the starting materials
Question #56
Transcription occurs in the nucleus or at the nucleoid.
A.   False
B.   True
Question #57
The emerging field of synthetic biology, while offering promising breakthroughs in medicine and energy production, is not without concern. Apprehension by ethicists is understandable since ________.
A.   the ability to create life from scratch raises a multitude of issues
B.   oil and gas prices will increase as a result of the decreased demand for fossil fuels
C.   medical insurance costs will likely skyrocket
D.   mutations in manufactured cells will cause problems for existing organisms
Question #58
The specific sequence of three bases in mRNA that encodes a particular amino acid is the _______ whereas the complementary sequence of three bases in tRNA that binds to the mRNA is best known as the _______.
A.   codon; anticodon
B.   codon; DNA triplet
C.   anticodon; codon
D.   gene; complement
Question #59
Eukaryotic chromosomes differ from bacterial chromosomes because only eukaryotes have ________.
A.   chromosomes in a nucleus
B.   All of the choices are correct
C.   several to many chromosomes
D.   histone proteins
E.   elongated, not circular, chromosomes
Question #60
When DNA is heated to 94 oC, the two strands will separate.
A.   True
B.   False
Question #61
Select the statement that best describes the purpose of gel electrophoresis.
A.   Gelelectrophoresis separates DNA fragments based on size.
B.   Gelelectrophoresis enables the amplification of specific DNA sequences.
C.   Gelelectrophoresis joins different segments of DNA.
D.   Gelelectrophoresis identifies specific segments of DNA.
E.   Gelelectrophoresis is able to cut DNA in very specific locations.
Question #62
Induced mutations result from errors in DNA replication.
A.   False
B.   True
Question #63
The lagging strand of DNA is made in small segments called Okazaki fragments, each one requiring a primer to begin synthesis, whereas the leading strand, synthesized continuously, does not require a primer to initiate the daughter strand.
A.   True
B.   False
Question #64
Synthetic biology is an emerging field of science whereby researchers are creating new molecules and organisms from an array of chemicals, rather than as a result of cellular reproduction.
A.   False
B.   True
Question #65
Repressible operons are normally turned on in the cell.
A.   False
B.   True
Question #66
Reverse transcriptase is used to make cDNA from an RNA template.
A.   False
B.   True
Question #67
The expression of genetic traits is the _______.
A.   proteome
B.   phenotype
C.   genome
D.   proteotype
E.   genotype
Question #68
Each nucleotide is composed of ________.
A.   two phosphates, one nitrogenous base, and one sugar
B.   one phosphate, one nitrogenous base, and two sugars
C.   two phosphates, one nitrogenous base, and two sugars
D.   one phosphate, one nitrogenous base, and one sugar
E.   one phosphate, two nitrogenous bases, and one sugar
Question #69
When bacteriophages invade bacterial cells, they leave behind DNA that is recognized by the bacteria as foreign and can be eliminated in the event of another attack, reminiscent of adaptive immunity. This natural process can be adapted in the lab so that a combination of guide DNA and nucleases facilitate the cutting of DNA at a precise location in any organism. Here we are describing ________.
A.   CRISPR
B.   metagenomics
C.   synthetic biology
D.   gene therapy
E.   recombinant DNA technology
Question #70
DNA replication proceeds in one direction around the bacterial chromosome.
A.   True
B.   False
Question #71
Choose the statement that best describes the role of restriction enzymes in genetic engineering.
A.   Restrictionenzymes are used to replicate specific sequences of DNA.
B.   Restrictionenzymes are used to cut DNA at specific locations.
C.   Restriction enzymesare used to join different segments of DNA.
D.   Restriction enzymes are used to purify DNA.
E.   Restrictionenzymes are used to sequence long stretches of DNA.

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