Microbiology 020 - General Microbiology » Winter 2022 » Lecture Exam 2 (Ch 5, 6, 7, 8)

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Question #1
Which of the following will not support viral cultivation?
A.   Continuous cell cultures
B.   All of thechoices will support viral cultivation.
C.   Embryonated bird eggs
D.   Primary cell cultures
E.   Live lab animals
Question #2
Persistent viruses that can reactivate periodically are _____.
A.   inclusion bodies
B.   syncytia
C.   chronic latent viruses
D.   cytopathic
E.   oncoviruses
Question #3
The envelope of enveloped viruses________.
A.   is only composed of host endomembrane
B.   None of the choices are correct
C.   does not contain spikes
D.   is obtained by viral budding or exocytosis
E.   is identical to the host plasma membrane
Question #4
Select the three choices that are the primary purposes of cultivating viruses.
A.   To research the biology of viruses To produce virusfor bioweapons use To produce chemicals such as alcohol and acetone
B.   To isolate viruses from clinical specimens To research the biology of viruses To prepare viruses for vaccines
C.   To produce virusfor bioweapons use To prepare viruses for vaccines To produce chemicals such as alcohol and acetone
D.   To produce virusfor bioweapons use To produce chemicals such as alcohol and acetone To isolate viruses from clinical specimens
Question #5
During lysogeny, an inactive prophage state occurs when the viral DNA is inserted into the ________.
A.   host DNA
B.   host cytoplasm
C.   host nucleolus
D.   host cell membrane
E.   host nucleus
Question #6
Select the three methods that are used to cultivate viruses.
A.   Use of animalinoculation Use of enriched broth media Use of enrichedagar media
B.   Use of enriched broth media Use of cellculture techniques Use of enrichedagar media
C.   Use of enriched broth media, Use of enrichedagar media, Inoculation ofembryonated eggs
D.   Inoculation ofembryonated eggs, Use of animalinoculation, Use of cellculture techniques
Question #7
A(n) _______ is the protein shell around the nucleic acid core of a virus.
A.   monolayer
B.   spike
C.   capsid
D.   capsomere
E.   envelope
Question #8
The process of dissolving the envelope and capsid to release the viral nucleic acid is _______.
A.   penetration
B.   uncoating
C.   synthesis
D.   assembly
E.   adsorption
Question #9
Which of the following is not a typical capsid shape?
A.   Icosahedron
B.   All of thechoices are capsid shapes.
C.   Helical
D.   Tetrahedral
E.   Complex
Question #10
A negative-sense RNA virus ________.
A.   must synthesize a positive RNA copy of its genome
B.   is a special form of tRNA used by viruses
C.   is ready for immediate translation
D.   always codes for RNA polymerase
E.   must synthesize a negative RNA copy of its genome
Question #11
Viruses that cause infection resulting in alternating periods of activity with symptoms and inactivity without symptoms are called _____.
A.   prions
B.   viroids
C.   oncogenic
D.   latent
E.   delta agents
Question #12
Viral tissue specificities are called _____.
A.   virions
B.   uncoating
C.   ranges
D.   receptacles
E.   tropisms
Question #13
Viruses that infect bacteria are specifically called _______.
A.   prions
B.   satellite viruses
C.   bacteriophages
D.   All of thechoices infect bacteria.
E.   viroids
Question #14
Infectious protein particles are called _______.
A.   spikes
B.   prions
C.   phages
D.   oncogenic viruses
E.   viroids
Question #15
Which of the following is incorrect about prophages?
A.   Formed when viral DNA enters the bacterial chromosome
B.   Replicated with host DNA and passed on to progeny
C.   Occur when temperate phages enter host cells
D.   Cause lysis of host cells
E.   Present when the virus is in lysogeny
Question #16
Host range is limited by________.
A.   type of host cell receptors on cell membrane
B.   age of the host cell
C.   type of nucleic acid in the virus
D.   size of the host cell
E.   All of the choices are correct
Question #17
Mammalian viruses capable of starting tumors are _____.
A.   cytopathic
B.   oncoviruses
C.   syncytia
D.   inclusion bodies
E.   chronic latent viruses
Question #18
Viral nucleic acids include which of the following?
A.   Double-stranded RNA
B.   Double-stranded DNA
C.   All of the choices are correct
D.   Single-stranded DNA
E.   Single-stranded RNA
Question #19
Lysogeny refers to ________.
A.   None of the choices are correct
B.   altering the host range of a virus
C.   virion exiting host cell
D.   viral genome inserting into bacterial host chromosome
E.   latent state of herpes infections
Question #20
When whole cells or large molecules in solution are engulfed by a cell, this endocytosis is specifically termed _______.
A.   pinocytosis
B.   facilitated diffusion
C.   exocytosis
D.   phagocytosis
E.   facilitated transport
Question #21
What type of media is used to demonstrate oxygen requirements of microbes?
A.   Sodium chloride
B.   Thioglycollate
C.   Blood agar
D.   None of the choices are correct.
E.   Sulfite polymyxin sulfadiazine
Question #22
Which of the following is not a major element of a microbial cell?
A.   Hydrogen
B.   Carbon
C.   Copper
D.   Oxygen
E.   Nitrogen
Question #23
Nutrient absorption is mediated by the________.
A.   cell wall
B.   peptidoglycan layer
C.   proteins in the periplasmic space
D.   nuclear membrane
E.   cell membrane
Question #24
An important mineral ion that is a component of chloroplasts and stabilizer of membranes and ribosomes is _______.
A.   zinc
B.   iron
C.   potassium
D.   calcium
E.   magnesium
Question #25
Select all of the cellular transport mechanisms that require energy.
A.   Facilitated diffusion and Carrier-mediated active transport
B.   Facilitated diffusion and Simple diffusion
C.   Simple diffusion and Bulk transport (phagocytosis, pinocytosis)
D.   Carrier-mediated active transport and Bulk transport (phagocytosis, pinocytosis)
Question #26
Facilitated diffusion is limited by________.
A.   the size of the cell
B.   size of the pores in the membrane
C.   carrier proteins in the membrane
D.   osmotic pressure
E.   substrate concentration
Question #27
Human pathogens fall into the group termed _______.
A.   halophiles
B.   psychrophiles
C.   acidophiles
D.   mesophiles
E.   thermophiles
Question #28
The movement of molecules from an area of high concentration to an area of lower concentration is called _______.
A.   diffusion
B.   endocytosis
C.   facilitated diffusion
D.   osmosis
E.   active transport
Question #29
The toxic superoxide ion is converted to harmless oxygen by two enzymes, ________.
A.   superoxide dismutase and hydrogen peroxidases
B.   superoxide dismutase and catalase
C.   catalase and hydrogen peroxidases
D.   superoxide dismutase and oxidase
E.   catalase and oxidase
Question #30
The term obligate refers to________.
A.   existing in a very narrow niche
B.   using chemicals for energy production
C.   using oxygen for metabolism
D.   using light for energy production
E.   the ability to exist in a wide range of conditions
Question #31
What compound is the most abundant in a cell?
A.   CH 4
B.   CO 2
C.   NH 3
D.   H 2O
E.   Glucose
Question #32
All of the following could find a location in or on body tissues suitable for growth except _______.
A.   anaerobes
B.   mesophiles
C.   psychrophiles
D.   facultative anaerobes
E.   capnophiles
Question #33
Bacterial biofilms stimulate the immune system differently than free (planktonic) bacteria.
A.   False
B.   True
Question #34
Aerobic respiration is an example of _______.
A.   photoautotrophy
B.   photosynthesis
C.   photoheterotrophy
D.   chemoheterotrophy
E.   methanoheterophy
Question #35
Most of the dry weight of a microbial cell is from _______.
A.   minerals
B.   inorganic compounds
C.   salts
D.   organic compounds
E.   water
Question #36
An organism that cannot tolerate an oxygen environment is a(n) _______.
A.   obligate aerobe
B.   obligate anaerobe
C.   microaerophile
D.   aerotolerant anaerobe
E.   facultative anaerobe
Question #37
Each of the following are denaturing agents except ________.
A.   high temperature
B.   All of the choices are correct.
C.   low temperature
D.   low pH
E.   high pH
Question #38
FAD, NAD, and coenzyme A are all _______ carriers.
A.   ATP
B.   electron
C.   None of the choices are correct.
D.   hydrogen andelectron
E.   hydrogen
Question #39
Exergonic reactions ________.
A.   include synthesis of large carbohydrates
B.   do not occur in anaerobic cellular respiration
C.   release energy
D.   only occur in heterotrophs
E.   occur when ADP binds to inorganic phosphate to form ATP
Question #40
The cell's metabolic reactions involve the participation of ________ that lower the activation energy needed for the initiation of a reaction.
A.   cofactors
B.   coenzymes
C.   enzymes
D.   vitamins
E.   ATP
Question #41
In a bacterial cell, the genes that code for enzymes that catalyze the hydrolysis of an unusual carbohydrate source would be turned off unless that carbohydrate was the only fuel source available in the media, in which case________.
A.   the genes coding for the hydrolytic enzymes would be repressed
B.   the genes coding for the hydrolytic enzymes would be induced
C.   the enzymes would be denatured
D.   the genes coding for the hydrolytic enzymes would be constitutive
Question #42
How many carbon dioxide molecules are produced by the complete aerobic breakdown of one glucose molecule?
A.   5
B.   3
C.   6
D.   4
E.   2
Question #43
Which of the following is not true of anaerobic respiration?
A.   Uses the same final electron acceptor as aerobic respiration
B.   Involves glycolysis
C.   Utilizes an electron transport system
D.   Generates some ATP
Question #44
Which of the following is not involved in the step that occurs between glycolysis and the Krebs cycle?
A.   Pyruvic acid accepts electrons from NADH
B.   CO2 is released
C.   NADH is formed
D.   Coenzyme A attaches to an acetyl group
Question #45
In bacterial cells, when glucose is completely oxidized by all the pathways of aerobic cellular respiration, what is the maximum number of ATP generated?
A.   2 ATP
B.   24 ATP
C.   38 ATP
D.   3 ATP
E.   42 ATP
Question #46
  
A.   phosphotransferases
B.   peptidases
C.   aminotransferases
D.   oxidoreductases
E.   ligases
Question #47
When glucose is broken down by glycolysis during bacterial fermentation, how many ATP are generated?
A.   3 ATP
B.   2 ATP
C.   38 ATP
D.   36 ATP
E.   24 ATP
Question #48
Each of the following is true of enzymes except ________.
A.   their active site is specific to the substrate
B.   All of the choices are true of enzymes.
C.   they can be used over and over
D.   they may or may not require cofactors
E.   they increase the initial energy required for a reaction to start
Question #49
The property of organisms to integrate catabolic and anabolic pathways to improve cell efficiency is called _______.
A.   amphibolism
B.   metabolism
C.   biosynthesis
D.   catabolism
E.   anabolism
Question #50
Fatty acids can be converted to acetyl-CoA for entering the Krebs cycle by the process of _______.
A.   deamination
B.     
C.   beta oxidation
D.   gluconeogenesis
E.   phosphorylation
F.   amination
Question #51
Reduction of nitrogen-oxygen ions, such as nitrate, by some bacteria is called _______.
A.   deamination
B.   denitrification
C.   nitrification
D.   aerobic respiration
E.   fermentation
Question #52
During which phase of cellular respiration is the majority of ATP formed?
A.   Electron transport
B.   Glycolysis
C.   Processing of pyruvic acid for the Krebs cycle
D.   Krebs cycle
E.   All phasesproduce the same number of ATP molecules.
Question #53
In addition to electrons, which of the following can also be transferred, exchanging energy in the process?
A.   Carbon dioxide
B.   Hydrogen ion (proton)
C.   ADP
D.   Glucose
E.   Carbon
Question #54
The _______ strand is synthesized continuously while the ________ strand is synthesized in a series of short fragments called Okazaki fragments.
A.   leading; lagging
B.   primary; secondary
C.   lagging; leading
D.   original; primary
Question #55
Mastery of synthetic biology will allow humans to ________.
A.   make new organisms using only chemicals as the starting materials
B.   All of the choices are possibilities from synthetic biology
C.   synthesize chemicals in cells that are missing due to a genetic anomaly
D.   construct complex molecules in situ that will target cancerous cells in tissues
E.   design alternative forms of energy production
Question #56
Transcription occurs in the nucleus or at the nucleoid.
A.   False
B.   True
Question #57
The emerging field of synthetic biology, while offering promising breakthroughs in medicine and energy production, is not without concern. Apprehension by ethicists is understandable since ________.
A.   mutations in manufactured cells will cause problems for existing organisms
B.   medical insurance costs will likely skyrocket
C.   oil and gas prices will increase as a result of the decreased demand for fossil fuels
D.   the ability to create life from scratch raises a multitude of issues
Question #58
The specific sequence of three bases in mRNA that encodes a particular amino acid is the _______ whereas the complementary sequence of three bases in tRNA that binds to the mRNA is best known as the _______.
A.   codon; DNA triplet
B.   anticodon; codon
C.   gene; complement
D.   codon; anticodon
Question #59
Eukaryotic chromosomes differ from bacterial chromosomes because only eukaryotes have ________.
A.   chromosomes in a nucleus
B.   several to many chromosomes
C.   All of the choices are correct
D.   elongated, not circular, chromosomes
E.   histone proteins
Question #60
When DNA is heated to 94 oC, the two strands will separate.
A.   False
B.   True
Question #61
Select the statement that best describes the purpose of gel electrophoresis.
A.   Gelelectrophoresis is able to cut DNA in very specific locations.
B.   Gelelectrophoresis identifies specific segments of DNA.
C.   Gelelectrophoresis separates DNA fragments based on size.
D.   Gelelectrophoresis joins different segments of DNA.
E.   Gelelectrophoresis enables the amplification of specific DNA sequences.
Question #62
Induced mutations result from errors in DNA replication.
A.   False
B.   True
Question #63
The lagging strand of DNA is made in small segments called Okazaki fragments, each one requiring a primer to begin synthesis, whereas the leading strand, synthesized continuously, does not require a primer to initiate the daughter strand.
A.   True
B.   False
Question #64
Synthetic biology is an emerging field of science whereby researchers are creating new molecules and organisms from an array of chemicals, rather than as a result of cellular reproduction.
A.   True
B.   False
Question #65
Repressible operons are normally turned on in the cell.
A.   True
B.   False
Question #66
Reverse transcriptase is used to make cDNA from an RNA template.
A.   False
B.   True
Question #67
The expression of genetic traits is the _______.
A.   phenotype
B.   genotype
C.   proteome
D.   proteotype
E.   genome
Question #68
Each nucleotide is composed of ________.
A.   one phosphate, two nitrogenous bases, and one sugar
B.   two phosphates, one nitrogenous base, and two sugars
C.   two phosphates, one nitrogenous base, and one sugar
D.   one phosphate, one nitrogenous base, and two sugars
E.   one phosphate, one nitrogenous base, and one sugar
Question #69
When bacteriophages invade bacterial cells, they leave behind DNA that is recognized by the bacteria as foreign and can be eliminated in the event of another attack, reminiscent of adaptive immunity. This natural process can be adapted in the lab so that a combination of guide DNA and nucleases facilitate the cutting of DNA at a precise location in any organism. Here we are describing ________.
A.   synthetic biology
B.   metagenomics
C.   gene therapy
D.   recombinant DNA technology
E.   CRISPR
Question #70
DNA replication proceeds in one direction around the bacterial chromosome.
A.   True
B.   False
Question #71
Choose the statement that best describes the role of restriction enzymes in genetic engineering.
A.   Restrictionenzymes are used to replicate specific sequences of DNA.
B.   Restriction enzymes are used to purify DNA.
C.   Restrictionenzymes are used to sequence long stretches of DNA.
D.   Restrictionenzymes are used to cut DNA at specific locations.
E.   Restriction enzymesare used to join different segments of DNA.

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