California State University, Fullerton » Biology » Biology 251 - Genetics » Spring 2021

Question #1

PCR is a DNA polymerization reaction in a tube. Unlike replication, PCR uses a ______________________.

A. an origin of replication.

B. an RNA primer.

C. helicase.

D. a DNA primer.

E. DNA gyrase.

Question #2

In the first round of PCR, the new DNA molecules generated are refered to as:

A. amplicons.

B. PCR products.

C. short products.

D. replicons.

E. long products.

Question #3

The double-stranded PCR products that are defined at their ends by the primers appear in round ________ and double every cycle thereafter.

A. 3

B. 1

C. 4

D. 2

Question #4

The enzyme used in PCR is a thermostable DNA polymerase called:

A. the Klenow fragment.

B. Taq polymerase.

C. DNA polymerase III.

D. DNA polymerase delta.

E. reverse transcriptase.

Question #5

The ________ step in PCR functions similarly to the origin of replication and initiator proteins in DNA replication.

A. extension

B. annealing

C. denaturation

D. elongation