Biology 251 - Genetics » Spring 2021 » Topic 9.1 Quiz
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Question #1
PCR is a DNA polymerization reaction in a tube. Unlike replication, PCR uses a ______________________.
A.
a DNA primer.
B.
an RNA primer.
C.
helicase.
D.
an origin of replication.
E.
DNA gyrase.
Question #2
In the first round of PCR, the new DNA molecules generated are refered to as:
A.
amplicons.
B.
replicons.
C.
short products.
D.
PCR products.
E.
long products.
Question #3
The double-stranded PCR products that are defined at their ends by the primers appear in round ________ and double every cycle thereafter.
A.
2
B.
3
C.
4
D.
1
Question #4
The enzyme used in PCR is a thermostable DNA polymerase called:
A.
the Klenow fragment.
B.
Taq polymerase.
C.
reverse transcriptase.
D.
DNA polymerase delta.
E.
DNA polymerase III.
Question #5
The ________ step in PCR functions similarly to the origin of replication and initiator proteins in DNA replication.
A.
denaturation
B.
elongation
C.
extension
D.
annealing
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