California State University, Fullerton » Biology » Biology 251 - Genetics » Spring 2021

Question #1

PCR is a DNA polymerization reaction in a tube. Unlike replication, PCR uses a ______________________.

A. a DNA primer.

B. DNA gyrase.

C. an origin of replication.

D. an RNA primer.

E. helicase.

Question #2

In the first round of PCR, the new DNA molecules generated are refered to as:

A. short products.

B. long products.

C. PCR products.

D. replicons.

E. amplicons.

Question #3

The double-stranded PCR products that are defined at their ends by the primers appear in round ________ and double every cycle thereafter.

A. 4

B. 2

C. 1

D. 3

Question #4

The enzyme used in PCR is a thermostable DNA polymerase called:

A. Taq polymerase.

B. DNA polymerase III.

C. the Klenow fragment.

D. DNA polymerase delta.

E. reverse transcriptase.

Question #5

The ________ step in PCR functions similarly to the origin of replication and initiator proteins in DNA replication.

A. extension

B. elongation

C. denaturation

D. annealing