California State University, Fullerton » Biology » Biology 251 - Genetics » Spring 2021

Question #1

PCR is a DNA polymerization reaction in a tube. Unlike replication, PCR uses a ______________________.

A. DNA gyrase.

B. a DNA primer.

C. an RNA primer.

D. helicase.

E. an origin of replication.

Question #2

In the first round of PCR, the new DNA molecules generated are refered to as:

A. PCR products.

B. long products.

C. amplicons.

D. short products.

E. replicons.

Question #3

The double-stranded PCR products that are defined at their ends by the primers appear in round ________ and double every cycle thereafter.

A. 1

B. 2

C. 3

D. 4

Question #4

The enzyme used in PCR is a thermostable DNA polymerase called:

A. the Klenow fragment.

B. Taq polymerase.

C. DNA polymerase delta.

D. DNA polymerase III.

E. reverse transcriptase.

Question #5

The ________ step in PCR functions similarly to the origin of replication and initiator proteins in DNA replication.

A. annealing

B. denaturation

C. elongation

D. extension