Biology 251 - Genetics » Spring 2021 » Topic 9.2 Quiz

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Question #1
Below is a double-stranded DNA sequence. Which part of the sequence is palindromic? 5' - ATGCTGGATCCTACC -3' 3'- TACGACCTAGGATGG -5'
A.   5'- CCTACC -3'
B.   5'- GGATCC - 3'
C.   5'- ATGCTG - 3'
D.   5'- ATCCTA - 3'
E.   5'- CTGGAT - 3'
Question #2
In the bacterial restriction system, a restriction endonuclease will cut _________ DNA.
A.   methylated
B.   unmethylated
Question #3
For cut DNA end represented below, what is the best description from the list? 5' - GATCC----------3' 3'- G----------5'
A.   4 base sticky end
B.   4 base, 3' overhang
C.   cohesive end
D.   4 base, 5' overhang
Question #4
DNA ligase can join any two DNAs with blunt ends, but not all DNAs that have overhangs.
A.   False
B.   True
Question #5
Plasmids sometimes include the feature of ___________ that is not one of the three essential features of a cloning vector, but facilitates identification of clones containing the desired insert.
A.   LacZ+
B.   a selectable marker
C.   ori
D.   a polylinker
E.   a multicloning site

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