Microbiology 020 - General Microbiology » Spring 2022 » Chapter 9 Quiz Growth.
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Question #1
Match the microbial growth requirements or preferences with the best description. Facultative halophiles
A.
Grow best at basic pH
B.
Tolerate, but do not require, high salt concentrations
C.
Grow best at temperatures below 15 Celsius, will die above 20 Celsius
D.
Grow at temperature above 85 Celsius
Question #2
Match the microbial growth requirements or preferences with the best description. Alkalinophiles
A.
Grow best at basic pH
B.
Responsible for most of the spoilage of refrigerated foods
C.
Grow under high atmospheric pressure
D.
Grow between 20 and 40 Celsius
Question #3
Match the microbial growth requirements or preferences with the best description. True psychrophiles
A.
Require high salt concentration
B.
Responsible for most of the spoilage of refrigerated foods
C.
Grow best at temperatures below 15 Celsius, will die above 20 Celsius
D.
Grow between 20 and 40 Celsius
Question #4
Match the microbial growth requirements or preferences with the best description. Hyperthermophiles
A.
Grow best at basic pH
B.
Grow at temperature above 85 Celsius
C.
Grow best at temperatures below 15 Celsius, will die above 20 Celsius
D.
Tolerate, but do not require, high salt concentrations
Question #5
Match the microbial growth requirements or preferences with the best description. Barophiles
A.
Responsible for most of the spoilage of refrigerated foods
B.
Require high salt concentration
C.
Grow between 20 and 40 Celsius
D.
Grow under high atmospheric pressure
Question #6
Match the microbial growth requirements or preferences with the best description. Mesophiles
A.
Grow best at basic pH
B.
Tolerate, but do not require, high salt concentrations
C.
Grow between 20 and 40 Celsius
D.
Grow best at temperatures below 15 Celsius, will die above 20 Celsius
Question #7
Match the microbial growth requirements or preferences with the best description. Psychrotrophs
A.
Tolerate, but do not require, high salt concentrations
B.
Require high salt concentration
C.
Grow best at basic pH
D.
Responsible for most of the spoilage of refrigerated foods
Question #8
Match the microbial growth requirements or preferences with the best description. Halophiles
A.
Grow best at temperatures below 15 Celsius, will die above 20 Celsius
B.
Grow between 20 and 40 Celsius
C.
Responsible for most of the spoilage of refrigerated foods
D.
Require high salt concentration
Question #9
Match the type of media with the best description: Defined media
A.
Provide anaerobic conditions for microbial growth
B.
Exact chemical composition is known
C.
Inhibits the growth of unwanted microbes and encourages the growth of other microbes
D.
Allows biologists to distinguish some microbes from others
Question #10
Match the type of media with the best description: Differential media
A.
Allows biologists to distinguish some microbes from others
B.
Inhibits the growth of unwanted microbes and encourages the growth of other microbes
C.
Provide anaerobic conditions for microbial growth
D.
Encourages or favors the growth of certain microbes found in low numbers
Question #11
Match the type of media with the best description: Selective media
A.
Provide anaerobic conditions for microbial growth
B.
Exact chemical composition is known
C.
Encourages or favors the growth of certain microbes found in low numbers
D.
Inhibits the growth of unwanted microbes and encourages the growth of other microbes
Question #12
Match the type of media with the best description: Reducing media
A.
Provide anaerobic conditions for microbial growth
B.
Exact chemical composition is known
C.
Encourages or favors the growth of certain microbes found in low numbers
D.
Allows biologists to distinguish some microbes from others
Question #13
Match the type of media with the best description: Enrichment media
A.
Encourages or favors the growth of certain microbes found in low numbers
B.
Inhibits the growth of unwanted microbes and encourages the growth of other microbes
C.
Provide anaerobic conditions for microbial growth
D.
Allows biologists to distinguish some microbes from others
Question #14
Match the phase or stage of microbial growth with the best description: Log phase
A.
Population is most susceptible to radiation and antimicrobial drugs
B.
The number of cells produced is equal to the number of cells that die.
C.
Cells are adjusting to their new environment
D.
Cells die at a faster rate than they are produced
Question #15
Match the phase or stage of microbial growth with the best description: Lag phase
A.
Cells die at a faster rate than they are produced
B.
Population is most susceptible to radiation and antimicrobial drugs
C.
The number of cells produced is equal to the number of cells that die.
D.
Cells are adjusting to their new environment
Question #16
Match the phase or stage of microbial growth with the best description: Decline phase
A.
Population is most susceptible to radiation and antimicrobial drugs
B.
The number of cells produced is equal to the number of cells that die.
C.
Cells die at a faster rate than they are produced
D.
Cells are adjusting to their new environment
Question #17
Match the phase or stage of microbial growth with the best description: Stationary phase
A.
Population is most susceptible to radiation and antimicrobial drugs
B.
The number of cells produced is equal to the number of cells that die.
C.
Cells are adjusting to their new environment
D.
Cells die at a faster rate than they are produced
Question #18
Which of the following is an indirect method to determine microbial growth?
A.
Turbidity
B.
Plate count
C.
Microscopic count
D.
Most probable number (MPN)
E.
All of these choices
F.
None of these choices
Question #19
Match the organism to the oxygen requirement: Obligate aerobes
A.
Cannot grow without oxygen
B.
Need low amount of oxygen, poisoned by high oxygen levels in air
C.
Does not need oxygen, but uses it and grows better when available
D.
Cannot grow in the presence of oxygen
Question #20
Match the organism to the oxygen requirement: Obligate anaerobes
A.
Cannot grow in the presence of oxygen
B.
Need low amount of oxygen, poisoned by high oxygen levels in air
C.
Does not need oxygen, but uses it and grows better when available
D.
Does not use oxygen, but not harmed by it
Question #21
Match the organism to the oxygen requirement: Microaerophiles
A.
Cannot grow without oxygen
B.
Need low amount of oxygen, poisoned by high oxygen levels in air
C.
Does not need oxygen, but uses it and grows better when available
D.
Does not use oxygen, but not harmed by it
Question #22
Match the organism to the oxygen requirement: Facultative anaerobe
A.
Does not need oxygen, but uses it and grows better when available
B.
Need low amount of oxygen, poisoned by high oxygen levels in air
C.
Cannot grow in the presence of oxygen
D.
Cannot grow without oxygen
Question #23
Match the organism to the oxygen requirement: Aerotolerant anaerobe
A.
Need low amount of oxygen, poisoned by high oxygen levels in air
B.
Cannot grow in the presence of oxygen
C.
Does not need oxygen, but uses it and grows better when available
D.
Does not use oxygen, but not harmed by it
Question #24
Which of the following enzymes breaks down hydrogen peroxide into water and oxygen?
A.
Lipase
B.
Protease
C.
Oxygenase
D.
Peroxidase
E.
Catalase
F.
Lactase
Question #25
When a bacterial cell is placed in a hypertonic solution it undergoes _____ .
A.
osmosis
B.
regeneration
C.
degeneration
D.
plasmolysis
Question #26
When an animal cell is placed in pure water it may undergo osmotic _____ , but bacterial cells are protected from this by the presence of their _____ _____ .
A.
lyses, plastic membrane
B.
phase, osmotic cell
C.
lyses, cell wall
D.
cycle, fatty cells
Question #27
In general, _____ solutions are usually the most damaging to bacterial cells.
A.
fermenting
B.
hypertonic
C.
endoplasmic
D.
glycolysis
Question #28
The most commonly used solidifying agent in microbiology is _____ , which is a polysaccharide derived from _____ algae.
A.
agar, blue
B.
agar, red
C.
salt, red
D.
resin, red
Question #29
This solidifying agent cannot be degraded by most _____ .
A.
virus
B.
catalase
C.
solvent
D.
bacteria
Question #30
It melts above 95 degrees Celsius, but does not solidify until it reaches _____ Celsius.
A.
100
B.
40
C.
60
D.
90
Question #31
Culture media must be sterile, contain appropriate _____ , and once inoculated needs to be incubated at the proper _____ .
A.
nutrient, condition
B.
mass, temperature
C.
weight, location
Question #32
The most commonly used method of isolation in microbiology is the _____ plate.
A.
catalase
B.
technique
C.
streak
Question #33
The goal of this technique is to obtain isolated _____ .
A.
colonies
B.
bacteria
C.
streak
Question #34
The time required for a bacterial cell to divide or for its population to _____ is called the _____ time.
A.
mutliply, degeneration
B.
solidify, turbidity
C.
double, generation
Question #35
Match the type of microbial oxygen requirement with the expected growth pattern on a semisolid agar deep: Obligate aerobe
A.
Growth in the middle section of tube
B.
Growth on bottom of tube
C.
Growth mostly on top of tube
D.
Grows throughout tube, but more growth on top
Question #36
Match the type of microbial oxygen requirement with the expected growth pattern on a semisolid agar deep: Obligate anaerobe
A.
Growth in the middle section of tube
B.
Growth throughout the tube
C.
Grows throughout tube, but more growth on top
D.
Growth on bottom of tube
Question #37
Match the type of microbial oxygen requirement with the expected growth pattern on a semisolid agar deep: Facultative anaerobe
A.
Growth mostly on top of tube
B.
Growth throughout the tube
C.
Grows throughout tube, but more growth on top
D.
Growth in the middle section of tube
Question #38
Match the type of microbial oxygen requirement with the expected growth pattern on a semisolid agar deep: Microaerophile
A.
Growth throughout the tube
B.
Growth in the middle section of tube
C.
Growth on bottom of tube
D.
Growth mostly on top of tube
Question #39
Match the type of microbial oxygen requirement with the expected growth pattern on a semisolid agar deep: Aerotolerant anaerobe
A.
Growth throughout the tube
B.
Grows throughout tube, but more growth on top
C.
Growth in the middle section of tube
D.
Growth on bottom of tube
Question #40
Which of the following are obligate anaerobes?
A.
Lactobacillus
B.
Campylobacter
C.
Escherichia
D.
Staphylococcus
E.
Clostridium
F.
Saccharomyces yeast
Question #41
Which of the following is a direct, statistical method commonly used to measure with 95% probability a range of the number of bacteria in a liquid culture?
A.
Turbidity
B.
Dry weight
C.
Metabolic activity
D.
Direct Microscopic Count
E.
Most Probable Number
F.
Filtration
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