Microbiology 020 - General Microbiology » Spring 2022 » Lab Quiz 1
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Question #1
Which of the following is used as a counterstain in a Gram stain?
A.
Congo Red
B.
Iodine
C.
Safranin
D.
Nigrosin
E.
Crystal violet
F.
Methylene blue
Question #2
Which of the following is used as a primary stain in an endospore stain stain?
A.
Maneval’s
B.
Crystal violet
C.
Malachite green
D.
Iodine
E.
Carbolfuchsin
F.
Methylene blue
Question #3
In a Gram stain, Gram positive cells usually retain the following stain:
A.
Crystal violet
B.
Malachite green
C.
Safranin
D.
Iodine
E.
Carbolfuchsin
F.
Congo red
Question #4
In a broth with bacterial growth, a layer of film floating on the top of the broth surface is called:
A.
Sediment
B.
Salinity
C.
Pellicle
D.
Motility
E.
Turbidity
F.
Pigment
Question #5
In a Gram stain, Gram negative cells usually retain the following stain:
A.
Iodine
B.
Methylene blue
C.
Congo red
D.
Safranin
E.
Crystal violet
F.
Maneval’s
Question #6
Which of the following is used a primary stain in an acid fast stain?
A.
Crystal violet
B.
Iodine
C.
Congo red
D.
Malachite green
E.
Saffranin
F.
Carbolfuchsin
Question #7
Acid-fast cells usually appear the following color after staining:
A.
Clear or colorless
B.
Black or blackish-blue
C.
Red or reddish-purple
D.
Orange
E.
Green or greenish-blue
F.
Yellow
Question #8
Bacterial endospores usually appear the following color after an endospore stain:
A.
Black
B.
Brown
C.
Red
D.
Clear or colorless
E.
White
F.
Green
Question #9
If you got a chemical in your eyes in the lab, the best course of action would be:
A.
Use a fire extinguisher
B.
Wipe your eyes with a cloth or paper towel
C.
Blink repeatedly until eyes stop burning
D.
Apply eye drops to each affected eye
E.
Remove your contact lenses (if applicable) and rinse your eyes in eye wash for at least 15 minutes
F.
Call 911 and wait for help to arrive
Question #10
Which of the following staining methods requires steaming a slide for 5 or more minutes?
A.
Flagellar stain
B.
Gram stain
C.
Simple stain
D.
None of these stains
E.
Both Endospore and Ziehl Neelsen acid-fast stains
F.
Negative stains
Question #11
When using a fire extinguisher, one should aim at the following part of a fire:
A.
Above the fire
B.
Base
C.
Outer periphery
D.
Middle
E.
Top
F.
None of these choices
Question #12
In a capsule stain, a bacterial capsule appears the following color:
A.
Clear
B.
Red or reddish-purple
C.
Brown
D.
Purple
E.
Green
F.
Pink
Question #13
The “cloudy” appearance of a broth with bacterial growth is referred to as:
A.
Turbidity
B.
Pigment
C.
Vortex
D.
Proglottid
E.
Pellicle
F.
Endospore
Question #14
The main difference between making a smear with a liquid culture and a solid culture is that you have to add 2-3 loopfuls of [] ____________________ when making a smear from a solid culture.
A.
iodine
B.
chlorine
C.
agar
D.
water
Question #15
One method that can be used to check for motility, using a regular slide and cover slip, is called a __________ [] mount.
A.
turbid
B.
dry
C.
direct
D.
wet
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