Microbiology 020 - General Microbiology » Spring 2022 » Lab Quiz 1
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Question #1
Which of the following is used as a counterstain in a Gram stain?
A.
Congo Red
B.
Crystal violet
C.
Iodine
D.
Safranin
E.
Nigrosin
F.
Methylene blue
Question #2
Which of the following is used as a primary stain in an endospore stain stain?
A.
Carbolfuchsin
B.
Iodine
C.
Maneval’s
D.
Crystal violet
E.
Methylene blue
F.
Malachite green
Question #3
In a Gram stain, Gram positive cells usually retain the following stain:
A.
Carbolfuchsin
B.
Malachite green
C.
Congo red
D.
Safranin
E.
Iodine
F.
Crystal violet
Question #4
In a broth with bacterial growth, a layer of film floating on the top of the broth surface is called:
A.
Sediment
B.
Salinity
C.
Pigment
D.
Motility
E.
Pellicle
F.
Turbidity
Question #5
In a Gram stain, Gram negative cells usually retain the following stain:
A.
Methylene blue
B.
Iodine
C.
Congo red
D.
Crystal violet
E.
Maneval’s
F.
Safranin
Question #6
Which of the following is used a primary stain in an acid fast stain?
A.
Saffranin
B.
Crystal violet
C.
Malachite green
D.
Carbolfuchsin
E.
Iodine
F.
Congo red
Question #7
Acid-fast cells usually appear the following color after staining:
A.
Clear or colorless
B.
Red or reddish-purple
C.
Green or greenish-blue
D.
Yellow
E.
Black or blackish-blue
F.
Orange
Question #8
Bacterial endospores usually appear the following color after an endospore stain:
A.
Red
B.
Clear or colorless
C.
White
D.
Black
E.
Green
F.
Brown
Question #9
If you got a chemical in your eyes in the lab, the best course of action would be:
A.
Call 911 and wait for help to arrive
B.
Apply eye drops to each affected eye
C.
Use a fire extinguisher
D.
Remove your contact lenses (if applicable) and rinse your eyes in eye wash for at least 15 minutes
E.
Blink repeatedly until eyes stop burning
F.
Wipe your eyes with a cloth or paper towel
Question #10
Which of the following staining methods requires steaming a slide for 5 or more minutes?
A.
Simple stain
B.
Gram stain
C.
Both Endospore and Ziehl Neelsen acid-fast stains
D.
Negative stains
E.
Flagellar stain
F.
None of these stains
Question #11
When using a fire extinguisher, one should aim at the following part of a fire:
A.
None of these choices
B.
Outer periphery
C.
Base
D.
Above the fire
E.
Middle
F.
Top
Question #12
In a capsule stain, a bacterial capsule appears the following color:
A.
Purple
B.
Pink
C.
Green
D.
Brown
E.
Red or reddish-purple
F.
Clear
Question #13
The “cloudy” appearance of a broth with bacterial growth is referred to as:
A.
Endospore
B.
Turbidity
C.
Pellicle
D.
Vortex
E.
Pigment
F.
Proglottid
Question #14
The main difference between making a smear with a liquid culture and a solid culture is that you have to add 2-3 loopfuls of [] ____________________ when making a smear from a solid culture.
A.
water
B.
chlorine
C.
iodine
D.
agar
Question #15
One method that can be used to check for motility, using a regular slide and cover slip, is called a __________ [] mount.
A.
turbid
B.
wet
C.
direct
D.
dry
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