Microbiology 020 - General Microbiology » Spring 2022 » Lab Quiz 1
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Question #1
Which of the following is used as a counterstain in a Gram stain?
A.
Methylene blue
B.
Crystal violet
C.
Iodine
D.
Nigrosin
E.
Congo Red
F.
Safranin
Question #2
Which of the following is used as a primary stain in an endospore stain stain?
A.
Methylene blue
B.
Crystal violet
C.
Maneval’s
D.
Iodine
E.
Carbolfuchsin
F.
Malachite green
Question #3
In a Gram stain, Gram positive cells usually retain the following stain:
A.
Malachite green
B.
Congo red
C.
Carbolfuchsin
D.
Iodine
E.
Safranin
F.
Crystal violet
Question #4
In a broth with bacterial growth, a layer of film floating on the top of the broth surface is called:
A.
Pigment
B.
Motility
C.
Salinity
D.
Turbidity
E.
Sediment
F.
Pellicle
Question #5
In a Gram stain, Gram negative cells usually retain the following stain:
A.
Iodine
B.
Maneval’s
C.
Safranin
D.
Crystal violet
E.
Congo red
F.
Methylene blue
Question #6
Which of the following is used a primary stain in an acid fast stain?
A.
Saffranin
B.
Carbolfuchsin
C.
Malachite green
D.
Iodine
E.
Crystal violet
F.
Congo red
Question #7
Acid-fast cells usually appear the following color after staining:
A.
Orange
B.
Clear or colorless
C.
Red or reddish-purple
D.
Black or blackish-blue
E.
Green or greenish-blue
F.
Yellow
Question #8
Bacterial endospores usually appear the following color after an endospore stain:
A.
White
B.
Black
C.
Green
D.
Clear or colorless
E.
Red
F.
Brown
Question #9
If you got a chemical in your eyes in the lab, the best course of action would be:
A.
Wipe your eyes with a cloth or paper towel
B.
Apply eye drops to each affected eye
C.
Use a fire extinguisher
D.
Call 911 and wait for help to arrive
E.
Blink repeatedly until eyes stop burning
F.
Remove your contact lenses (if applicable) and rinse your eyes in eye wash for at least 15 minutes
Question #10
Which of the following staining methods requires steaming a slide for 5 or more minutes?
A.
Simple stain
B.
Both Endospore and Ziehl Neelsen acid-fast stains
C.
Flagellar stain
D.
Gram stain
E.
Negative stains
F.
None of these stains
Question #11
When using a fire extinguisher, one should aim at the following part of a fire:
A.
Outer periphery
B.
Middle
C.
Base
D.
Above the fire
E.
None of these choices
F.
Top
Question #12
In a capsule stain, a bacterial capsule appears the following color:
A.
Pink
B.
Brown
C.
Green
D.
Red or reddish-purple
E.
Purple
F.
Clear
Question #13
The “cloudy” appearance of a broth with bacterial growth is referred to as:
A.
Turbidity
B.
Endospore
C.
Pellicle
D.
Vortex
E.
Proglottid
F.
Pigment
Question #14
The main difference between making a smear with a liquid culture and a solid culture is that you have to add 2-3 loopfuls of [] ____________________ when making a smear from a solid culture.
A.
iodine
B.
water
C.
chlorine
D.
agar
Question #15
One method that can be used to check for motility, using a regular slide and cover slip, is called a __________ [] mount.
A.
wet
B.
turbid
C.
direct
D.
dry
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