Microbiology 020 - General Microbiology » Spring 2022 » Lab Quiz 1
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Question #1
Which of the following is used as a counterstain in a Gram stain?
A.
Methylene blue
B.
Congo Red
C.
Nigrosin
D.
Iodine
E.
Safranin
F.
Crystal violet
Question #2
Which of the following is used as a primary stain in an endospore stain stain?
A.
Carbolfuchsin
B.
Methylene blue
C.
Crystal violet
D.
Iodine
E.
Malachite green
F.
Maneval’s
Question #3
In a Gram stain, Gram positive cells usually retain the following stain:
A.
Malachite green
B.
Safranin
C.
Iodine
D.
Crystal violet
E.
Congo red
F.
Carbolfuchsin
Question #4
In a broth with bacterial growth, a layer of film floating on the top of the broth surface is called:
A.
Pigment
B.
Turbidity
C.
Pellicle
D.
Motility
E.
Sediment
F.
Salinity
Question #5
In a Gram stain, Gram negative cells usually retain the following stain:
A.
Methylene blue
B.
Safranin
C.
Maneval’s
D.
Congo red
E.
Iodine
F.
Crystal violet
Question #6
Which of the following is used a primary stain in an acid fast stain?
A.
Malachite green
B.
Congo red
C.
Carbolfuchsin
D.
Iodine
E.
Crystal violet
F.
Saffranin
Question #7
Acid-fast cells usually appear the following color after staining:
A.
Yellow
B.
Green or greenish-blue
C.
Red or reddish-purple
D.
Clear or colorless
E.
Orange
F.
Black or blackish-blue
Question #8
Bacterial endospores usually appear the following color after an endospore stain:
A.
Brown
B.
Red
C.
White
D.
Clear or colorless
E.
Green
F.
Black
Question #9
If you got a chemical in your eyes in the lab, the best course of action would be:
A.
Blink repeatedly until eyes stop burning
B.
Call 911 and wait for help to arrive
C.
Apply eye drops to each affected eye
D.
Remove your contact lenses (if applicable) and rinse your eyes in eye wash for at least 15 minutes
E.
Wipe your eyes with a cloth or paper towel
F.
Use a fire extinguisher
Question #10
Which of the following staining methods requires steaming a slide for 5 or more minutes?
A.
Both Endospore and Ziehl Neelsen acid-fast stains
B.
Flagellar stain
C.
Negative stains
D.
Gram stain
E.
None of these stains
F.
Simple stain
Question #11
When using a fire extinguisher, one should aim at the following part of a fire:
A.
Top
B.
Middle
C.
Base
D.
None of these choices
E.
Above the fire
F.
Outer periphery
Question #12
In a capsule stain, a bacterial capsule appears the following color:
A.
Green
B.
Brown
C.
Red or reddish-purple
D.
Purple
E.
Pink
F.
Clear
Question #13
The “cloudy” appearance of a broth with bacterial growth is referred to as:
A.
Turbidity
B.
Pigment
C.
Endospore
D.
Pellicle
E.
Proglottid
F.
Vortex
Question #14
The main difference between making a smear with a liquid culture and a solid culture is that you have to add 2-3 loopfuls of [] ____________________ when making a smear from a solid culture.
A.
water
B.
iodine
C.
chlorine
D.
agar
Question #15
One method that can be used to check for motility, using a regular slide and cover slip, is called a __________ [] mount.
A.
dry
B.
turbid
C.
wet
D.
direct
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