Biology 380 - Cell Biology » Summer 2022 » Quiz 1, Ch 1 and 8

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Question #1
Which of the following methods would be the most suitable to assess the relative purity of a protein in a sample you have prepared?
A.   In situ hybridization
B.   Gel-filtration chromatography
C.   Ion-exchange chromatography
D.   Genomic library
E.   Gel electrophoresis
Question #2
Place the following steps in the correct order for cloning a single gene using bacteria. A. Mix the linear plasmid with the DNA fragment to be cloned B. Cut plasmid with restriction enzyme C. Purify recombinant plasmd from bacteria D. Add DNA ligase to mixture E. Introduce recombinant plasmid into bacterial cell
A.   C, B, A, E, D
B.   C, A, B, D, E
C.   B, D, A, C, E
D.   B, A, D, E, C
E.   D, A, E, C, B
Question #3
All cells share the following features except:
A.   All cells store their hereditary information in DNA
B.   All cells require ATP
C.   All cells translate RNA into protein the same way
D.   All cells are enclosed in a cell wall
E.   All cells use proteins as catalysts
F.   All cells replicate their hereditary information by templated polymerization

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