Biology 380 - Cell Biology » Summer 2022 » Quiz 1, Ch 1 and 8

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Question #1
Which of the following methods would be the most suitable to assess the relative purity of a protein in a sample you have prepared?
A.   Ion-exchange chromatography
B.   In situ hybridization
C.   Gel-filtration chromatography
D.   Gel electrophoresis
E.   Genomic library
Question #2
Place the following steps in the correct order for cloning a single gene using bacteria. A. Mix the linear plasmid with the DNA fragment to be cloned B. Cut plasmid with restriction enzyme C. Purify recombinant plasmd from bacteria D. Add DNA ligase to mixture E. Introduce recombinant plasmid into bacterial cell
A.   D, A, E, C, B
B.   B, A, D, E, C
C.   C, A, B, D, E
D.   B, D, A, C, E
E.   C, B, A, E, D
Question #3
All cells share the following features except:
A.   All cells require ATP
B.   All cells translate RNA into protein the same way
C.   All cells replicate their hereditary information by templated polymerization
D.   All cells use proteins as catalysts
E.   All cells store their hereditary information in DNA
F.   All cells are enclosed in a cell wall

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