Prince George Community College » Biology » Bio 2010 - Microbiology » Summer 2022

Question #1

__________ is the primary stain used in the endospore stain.

A. carbolfushin

B. safranin

C. malachite green

D. crystal violet

Question #2

Place the steps of the Gram stain in the correct order: 1. 95% acetone alcohol 2. crystal violet 3. safranin 4. iodine

A. 2-4-1-3

B. 4-3-2-1

C. 2-1-4-3

D. 1-3-2-4

E. 1-2-3-4

Question #3

The primary stain used in the Gram stain is

A. iodine

B. acetone-alcohol

C. crystal violet

D. safranin

Question #4

The secondary stain used in the acid-fast stain

A. crystal violet

B. carbolfushin

C. safranin

D. methylene blue

Question #5

Which of the following does not use a destain (decolorizing) agent?

A. acid-fast stain

B. Gram stain

C. endospore stain

Question #6

Which of the following types of stains utilizes a mordant to prevent the release of the primary stain?

A. Gram stain

B. acid-fast stain

C. endospore stain

D. simple stain

Question #7

Which of these bacterial genera are endospore-formers? 1. Clostridium 2. Staphylococcus 3. Mycobacterium 4. Bacillus

A. 2 and 3

B. 3 and 4

C. 1 and 2

D. 1 and 4