Bio 2010 - Microbiology » Summer 2022 » Aseptic Transfer Technique

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Question #1
What is the goal of aseptic transfer technique?
A.   to prevent contamination
B.   none of these
C.   more than one of the above is correct
D.   to allow contamination
E.   to reduce contamination
Question #2
Why is it important to flame the test tubes before and after transferring bacteria into fresh, sterile culture media?
A.   more than one of the above is correct
B.   none of these
C.   prevent contaminating the second culture tube
D.   to seal the plastic lid on the tube
E.   clean the tube for use in the future
Question #3
Which of the following best describes contamination?
A.   more than one of the above is correct
B.   presence of oxygen
C.   presence of carbon dioxide
D.   presence of microorganisms
E.   none of these
F.   oxygen free
Question #4
You and your partner were talking during your aseptic transfer activity. You cannot remember if you flamed your flame loop before you opened your first test tube. What should you do in order to avoid contamination?
A.   nothing, the last person flamed it
B.   get a different flame loop
C.   nothing, bacteria cannot grow on the metal loop
D.   flame it again just to be sure.

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