Bio 2010 - Microbiology » Summer 2022 » Aseptic Transfer Technique

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Question #1
What is the goal of aseptic transfer technique?
A.   to allow contamination
B.   to prevent contamination
C.   to reduce contamination
D.   more than one of the above is correct
E.   none of these
Question #2
Why is it important to flame the test tubes before and after transferring bacteria into fresh, sterile culture media?
A.   prevent contaminating the second culture tube
B.   more than one of the above is correct
C.   to seal the plastic lid on the tube
D.   clean the tube for use in the future
E.   none of these
Question #3
Which of the following best describes contamination?
A.   more than one of the above is correct
B.   none of these
C.   oxygen free
D.   presence of oxygen
E.   presence of carbon dioxide
F.   presence of microorganisms
Question #4
You and your partner were talking during your aseptic transfer activity. You cannot remember if you flamed your flame loop before you opened your first test tube. What should you do in order to avoid contamination?
A.   nothing, bacteria cannot grow on the metal loop
B.   flame it again just to be sure.
C.   nothing, the last person flamed it
D.   get a different flame loop

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