Bio 2010 - Microbiology » Summer 2022 » Aseptic Transfer Technique

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Question #1
What is the goal of aseptic transfer technique?
A.   to allow contamination
B.   to reduce contamination
C.   more than one of the above is correct
D.   to prevent contamination
E.   none of these
Question #2
Why is it important to flame the test tubes before and after transferring bacteria into fresh, sterile culture media?
A.   clean the tube for use in the future
B.   to seal the plastic lid on the tube
C.   more than one of the above is correct
D.   prevent contaminating the second culture tube
E.   none of these
Question #3
Which of the following best describes contamination?
A.   none of these
B.   presence of oxygen
C.   oxygen free
D.   more than one of the above is correct
E.   presence of carbon dioxide
F.   presence of microorganisms
Question #4
You and your partner were talking during your aseptic transfer activity. You cannot remember if you flamed your flame loop before you opened your first test tube. What should you do in order to avoid contamination?
A.   flame it again just to be sure.
B.   nothing, bacteria cannot grow on the metal loop
C.   get a different flame loop
D.   nothing, the last person flamed it

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