Bio 2010 - Microbiology » Summer 2022 » Lab Exam 1

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Question #1
Choose the term below, that BEST completes the following statement. As magnification increases, the depth-of-field:
A.   decreases
B.   stays the same
C.   there is no relationship between these two
D.   increases
Question #2
Choose the term below, that BEST completes the following statement. As magnification increases, the resolution:
A.   stays the same
B.   there is no relationship between these two
C.   increases
D.   decreases
Question #3
Once inoculated with bacteria, how should agar Petri plates be incubated?
A.   right side up
B.   none of these
C.   upside down
D.   sideways
E.   more than one of the above is correct
Question #4
Which of the following best describes contamination?
A.   presence of carbon dioxide
B.   more than one of choices are correct
C.   presence of oxygen
D.   presence of microorganisms
E.   oxygen free
Question #5
Which of the following terms regarding the presence of microorganisms means "everywhere"?
A.   ubiquitous
B.   sporadic
C.   tropical
D.   claryvoyant
Question #6
Which of the following is the correct temperature for incubating bacteria taken from a human small intestine?
A.   25oC
B.   10oC
C.   20oC
D.   37oC
Question #7
Which of the following is the Gram's primary stain?
A.   Loeffler's methylene blue
B.   Safranin
C.   Acetone- Alcohol
D.   Carbolfuchsin
E.   Crystal violet
Question #8
Which of the following is the Gram's trapping agent?
A.   Safranin
B.   Crystal violet
C.   Loeffler's methylene blue
D.   Iodine
E.   Acetone- Alcohol
Question #9
Which of the following is the most probable genus and species of bacteria indicated by the letter “A”?
A.   Neisseria sicca
B.   Mycobacterium smegmatis
C.   Escherichia coli
D.   Bacillus subtilis
E.   Staphylococcus epidermidis
Question #10
Which compound do Ziehl-Neelsen acid-fast positive stained cells have in their cell walls?
A.   lactic acid
B.   peptydoglycan
C.   crystal violet
D.   mycolic acid
Question #11
Which of the following is the substrate found in Skim Milk Agar?
A.   triglyceride
B.   gelatin
C.   casein
D.   starch
Question #12
Which of the following is the Ziehl-Neelsen acid fast primary stain?
A.   carbolfuchsin
B.   safranin
C.   acid alcohol
D.   none of these
E.   crystal violet
Question #13
From the above image, what is the name of the green cells?
A.   vegetative cells
B.   spores
C.   cysts
D.   endospores
Question #14
Which of the following is the Shaeffer-Fulton endospore bleach or destain?
A.   acid alcohol
B.   carbolfuchsin
C.   none of these
D.   crystal violet
E.   safranin
Question #15
Which of the bacteria genus and species below produce pigmented colonies when plated on agar?
A.   none of these
B.   Serratia marcescens
C.   Escherichia coli
D.   Bacillus subtilis
E.   Staphylococcus aureus
Question #16
From our microbial control lab experiments, which of the following bacteria genus and species should have displayed the highest thermal death point?
A.   Neisseria sicca
B.   Escherichia coli
C.   Staphylococcus epidermids
D.   Bacillus subtilis
Question #17
Which of the following exoenzymes does the Spirit Blue Agar test for?
A.   Gelatinase
B.   Lipase
C.   Caseinase
D.   Amylase
E.   None of these
Question #18
Which of the following is a positive Starch Agar final product?
A.   glucose
B.   nucleotides
C.   fatty acids
D.   amino acids
E.   fructose
Question #19
Which of the following is the BEST reason to flame your flame loop between each Streak-for-Isolation sector?
A.   It carves a path in the agar that promotes isolation
B.   it reduces the number of bacteria as we move them from sector I to sector IV
C.   it is good practice for other lab related techniques
D.   it increases the number of bacteria as we move them from sector I to sector IV

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