Bio 2010 - Microbiology » Summer 2022 » Lab Exam 1

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Question #1
Choose the term below, that BEST completes the following statement. As magnification increases, the depth-of-field:
A.   decreases
B.   stays the same
C.   increases
D.   there is no relationship between these two
Question #2
Choose the term below, that BEST completes the following statement. As magnification increases, the resolution:
A.   increases
B.   there is no relationship between these two
C.   decreases
D.   stays the same
Question #3
Once inoculated with bacteria, how should agar Petri plates be incubated?
A.   right side up
B.   more than one of the above is correct
C.   none of these
D.   upside down
E.   sideways
Question #4
Which of the following best describes contamination?
A.   oxygen free
B.   more than one of choices are correct
C.   presence of microorganisms
D.   presence of oxygen
E.   presence of carbon dioxide
Question #5
Which of the following terms regarding the presence of microorganisms means "everywhere"?
A.   sporadic
B.   claryvoyant
C.   tropical
D.   ubiquitous
Question #6
Which of the following is the correct temperature for incubating bacteria taken from a human small intestine?
A.   10oC
B.   37oC
C.   25oC
D.   20oC
Question #7
Which of the following is the Gram's primary stain?
A.   Carbolfuchsin
B.   Crystal violet
C.   Acetone- Alcohol
D.   Safranin
E.   Loeffler's methylene blue
Question #8
Which of the following is the Gram's trapping agent?
A.   Safranin
B.   Acetone- Alcohol
C.   Crystal violet
D.   Iodine
E.   Loeffler's methylene blue
Question #9
Which of the following is the most probable genus and species of bacteria indicated by the letter “A”?
A.   Mycobacterium smegmatis
B.   Bacillus subtilis
C.   Escherichia coli
D.   Staphylococcus epidermidis
E.   Neisseria sicca
Question #10
Which compound do Ziehl-Neelsen acid-fast positive stained cells have in their cell walls?
A.   crystal violet
B.   mycolic acid
C.   lactic acid
D.   peptydoglycan
Question #11
Which of the following is the substrate found in Skim Milk Agar?
A.   starch
B.   triglyceride
C.   casein
D.   gelatin
Question #12
Which of the following is the Ziehl-Neelsen acid fast primary stain?
A.   carbolfuchsin
B.   acid alcohol
C.   safranin
D.   none of these
E.   crystal violet
Question #13
From the above image, what is the name of the green cells?
A.   spores
B.   endospores
C.   cysts
D.   vegetative cells
Question #14
Which of the following is the Shaeffer-Fulton endospore bleach or destain?
A.   none of these
B.   crystal violet
C.   safranin
D.   carbolfuchsin
E.   acid alcohol
Question #15
Which of the bacteria genus and species below produce pigmented colonies when plated on agar?
A.   Escherichia coli
B.   Staphylococcus aureus
C.   Bacillus subtilis
D.   none of these
E.   Serratia marcescens
Question #16
From our microbial control lab experiments, which of the following bacteria genus and species should have displayed the highest thermal death point?
A.   Bacillus subtilis
B.   Neisseria sicca
C.   Escherichia coli
D.   Staphylococcus epidermids
Question #17
Which of the following exoenzymes does the Spirit Blue Agar test for?
A.   Lipase
B.   Gelatinase
C.   None of these
D.   Caseinase
E.   Amylase
Question #18
Which of the following is a positive Starch Agar final product?
A.   nucleotides
B.   amino acids
C.   fructose
D.   glucose
E.   fatty acids
Question #19
Which of the following is the BEST reason to flame your flame loop between each Streak-for-Isolation sector?
A.   It carves a path in the agar that promotes isolation
B.   it is good practice for other lab related techniques
C.   it increases the number of bacteria as we move them from sector I to sector IV
D.   it reduces the number of bacteria as we move them from sector I to sector IV

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