Bio 2010 - Microbiology » Summer 2022 » Lab Exam 1

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Question #1
Choose the term below, that BEST completes the following statement. As magnification increases, the depth-of-field:
A.   stays the same
B.   increases
C.   there is no relationship between these two
D.   decreases
Question #2
Choose the term below, that BEST completes the following statement. As magnification increases, the resolution:
A.   decreases
B.   there is no relationship between these two
C.   increases
D.   stays the same
Question #3
Once inoculated with bacteria, how should agar Petri plates be incubated?
A.   more than one of the above is correct
B.   upside down
C.   right side up
D.   none of these
E.   sideways
Question #4
Which of the following best describes contamination?
A.   presence of oxygen
B.   presence of carbon dioxide
C.   presence of microorganisms
D.   oxygen free
E.   more than one of choices are correct
Question #5
Which of the following terms regarding the presence of microorganisms means "everywhere"?
A.   sporadic
B.   tropical
C.   claryvoyant
D.   ubiquitous
Question #6
Which of the following is the correct temperature for incubating bacteria taken from a human small intestine?
A.   20oC
B.   37oC
C.   10oC
D.   25oC
Question #7
Which of the following is the Gram's primary stain?
A.   Loeffler's methylene blue
B.   Safranin
C.   Acetone- Alcohol
D.   Crystal violet
E.   Carbolfuchsin
Question #8
Which of the following is the Gram's trapping agent?
A.   Iodine
B.   Loeffler's methylene blue
C.   Crystal violet
D.   Safranin
E.   Acetone- Alcohol
Question #9
Which of the following is the most probable genus and species of bacteria indicated by the letter “A”?
A.   Neisseria sicca
B.   Escherichia coli
C.   Mycobacterium smegmatis
D.   Bacillus subtilis
E.   Staphylococcus epidermidis
Question #10
Which compound do Ziehl-Neelsen acid-fast positive stained cells have in their cell walls?
A.   crystal violet
B.   peptydoglycan
C.   mycolic acid
D.   lactic acid
Question #11
Which of the following is the substrate found in Skim Milk Agar?
A.   triglyceride
B.   casein
C.   gelatin
D.   starch
Question #12
Which of the following is the Ziehl-Neelsen acid fast primary stain?
A.   none of these
B.   safranin
C.   crystal violet
D.   carbolfuchsin
E.   acid alcohol
Question #13
From the above image, what is the name of the green cells?
A.   spores
B.   vegetative cells
C.   cysts
D.   endospores
Question #14
Which of the following is the Shaeffer-Fulton endospore bleach or destain?
A.   crystal violet
B.   safranin
C.   acid alcohol
D.   carbolfuchsin
E.   none of these
Question #15
Which of the bacteria genus and species below produce pigmented colonies when plated on agar?
A.   Bacillus subtilis
B.   none of these
C.   Escherichia coli
D.   Serratia marcescens
E.   Staphylococcus aureus
Question #16
From our microbial control lab experiments, which of the following bacteria genus and species should have displayed the highest thermal death point?
A.   Neisseria sicca
B.   Staphylococcus epidermids
C.   Escherichia coli
D.   Bacillus subtilis
Question #17
Which of the following exoenzymes does the Spirit Blue Agar test for?
A.   None of these
B.   Caseinase
C.   Amylase
D.   Lipase
E.   Gelatinase
Question #18
Which of the following is a positive Starch Agar final product?
A.   glucose
B.   nucleotides
C.   fructose
D.   fatty acids
E.   amino acids
Question #19
Which of the following is the BEST reason to flame your flame loop between each Streak-for-Isolation sector?
A.   it is good practice for other lab related techniques
B.   it reduces the number of bacteria as we move them from sector I to sector IV
C.   it increases the number of bacteria as we move them from sector I to sector IV
D.   It carves a path in the agar that promotes isolation

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