Bio 2010 - Microbiology » Summer 2022 » Lab Exam 1

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Question #1
Choose the term below, that BEST completes the following statement. As magnification increases, the depth-of-field:
A.   there is no relationship between these two
B.   decreases
C.   stays the same
D.   increases
Question #2
Choose the term below, that BEST completes the following statement. As magnification increases, the resolution:
A.   there is no relationship between these two
B.   increases
C.   stays the same
D.   decreases
Question #3
Once inoculated with bacteria, how should agar Petri plates be incubated?
A.   sideways
B.   right side up
C.   none of these
D.   more than one of the above is correct
E.   upside down
Question #4
Which of the following best describes contamination?
A.   presence of carbon dioxide
B.   presence of microorganisms
C.   presence of oxygen
D.   oxygen free
E.   more than one of choices are correct
Question #5
Which of the following terms regarding the presence of microorganisms means "everywhere"?
A.   tropical
B.   ubiquitous
C.   claryvoyant
D.   sporadic
Question #6
Which of the following is the correct temperature for incubating bacteria taken from a human small intestine?
A.   25oC
B.   37oC
C.   10oC
D.   20oC
Question #7
Which of the following is the Gram's primary stain?
A.   Loeffler's methylene blue
B.   Carbolfuchsin
C.   Crystal violet
D.   Acetone- Alcohol
E.   Safranin
Question #8
Which of the following is the Gram's trapping agent?
A.   Crystal violet
B.   Iodine
C.   Acetone- Alcohol
D.   Safranin
E.   Loeffler's methylene blue
Question #9
Which of the following is the most probable genus and species of bacteria indicated by the letter “A”?
A.   Neisseria sicca
B.   Bacillus subtilis
C.   Mycobacterium smegmatis
D.   Escherichia coli
E.   Staphylococcus epidermidis
Question #10
Which compound do Ziehl-Neelsen acid-fast positive stained cells have in their cell walls?
A.   lactic acid
B.   crystal violet
C.   peptydoglycan
D.   mycolic acid
Question #11
Which of the following is the substrate found in Skim Milk Agar?
A.   casein
B.   gelatin
C.   triglyceride
D.   starch
Question #12
Which of the following is the Ziehl-Neelsen acid fast primary stain?
A.   acid alcohol
B.   none of these
C.   carbolfuchsin
D.   crystal violet
E.   safranin
Question #13
From the above image, what is the name of the green cells?
A.   spores
B.   vegetative cells
C.   endospores
D.   cysts
Question #14
Which of the following is the Shaeffer-Fulton endospore bleach or destain?
A.   carbolfuchsin
B.   crystal violet
C.   none of these
D.   acid alcohol
E.   safranin
Question #15
Which of the bacteria genus and species below produce pigmented colonies when plated on agar?
A.   Serratia marcescens
B.   Staphylococcus aureus
C.   none of these
D.   Escherichia coli
E.   Bacillus subtilis
Question #16
From our microbial control lab experiments, which of the following bacteria genus and species should have displayed the highest thermal death point?
A.   Staphylococcus epidermids
B.   Escherichia coli
C.   Bacillus subtilis
D.   Neisseria sicca
Question #17
Which of the following exoenzymes does the Spirit Blue Agar test for?
A.   Amylase
B.   Caseinase
C.   None of these
D.   Gelatinase
E.   Lipase
Question #18
Which of the following is a positive Starch Agar final product?
A.   glucose
B.   fatty acids
C.   amino acids
D.   nucleotides
E.   fructose
Question #19
Which of the following is the BEST reason to flame your flame loop between each Streak-for-Isolation sector?
A.   it is good practice for other lab related techniques
B.   it reduces the number of bacteria as we move them from sector I to sector IV
C.   It carves a path in the agar that promotes isolation
D.   it increases the number of bacteria as we move them from sector I to sector IV

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