Bio 2010 - Microbiology » Summer 2022 » Lab Exam 1

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Question #1
Choose the term below, that BEST completes the following statement. As magnification increases, the depth-of-field:
A.   decreases
B.   there is no relationship between these two
C.   increases
D.   stays the same
Question #2
Choose the term below, that BEST completes the following statement. As magnification increases, the resolution:
A.   decreases
B.   increases
C.   there is no relationship between these two
D.   stays the same
Question #3
Once inoculated with bacteria, how should agar Petri plates be incubated?
A.   upside down
B.   right side up
C.   sideways
D.   more than one of the above is correct
E.   none of these
Question #4
Which of the following best describes contamination?
A.   presence of oxygen
B.   oxygen free
C.   more than one of choices are correct
D.   presence of carbon dioxide
E.   presence of microorganisms
Question #5
Which of the following terms regarding the presence of microorganisms means "everywhere"?
A.   sporadic
B.   ubiquitous
C.   tropical
D.   claryvoyant
Question #6
Which of the following is the correct temperature for incubating bacteria taken from a human small intestine?
A.   20oC
B.   37oC
C.   25oC
D.   10oC
Question #7
Which of the following is the Gram's primary stain?
A.   Safranin
B.   Crystal violet
C.   Carbolfuchsin
D.   Loeffler's methylene blue
E.   Acetone- Alcohol
Question #8
Which of the following is the Gram's trapping agent?
A.   Safranin
B.   Acetone- Alcohol
C.   Loeffler's methylene blue
D.   Crystal violet
E.   Iodine
Question #9
Which of the following is the most probable genus and species of bacteria indicated by the letter “A”?
A.   Mycobacterium smegmatis
B.   Escherichia coli
C.   Staphylococcus epidermidis
D.   Bacillus subtilis
E.   Neisseria sicca
Question #10
Which compound do Ziehl-Neelsen acid-fast positive stained cells have in their cell walls?
A.   peptydoglycan
B.   crystal violet
C.   lactic acid
D.   mycolic acid
Question #11
Which of the following is the substrate found in Skim Milk Agar?
A.   gelatin
B.   casein
C.   triglyceride
D.   starch
Question #12
Which of the following is the Ziehl-Neelsen acid fast primary stain?
A.   acid alcohol
B.   crystal violet
C.   carbolfuchsin
D.   safranin
E.   none of these
Question #13
From the above image, what is the name of the green cells?
A.   vegetative cells
B.   cysts
C.   endospores
D.   spores
Question #14
Which of the following is the Shaeffer-Fulton endospore bleach or destain?
A.   safranin
B.   none of these
C.   crystal violet
D.   acid alcohol
E.   carbolfuchsin
Question #15
Which of the bacteria genus and species below produce pigmented colonies when plated on agar?
A.   Escherichia coli
B.   Bacillus subtilis
C.   Serratia marcescens
D.   none of these
E.   Staphylococcus aureus
Question #16
From our microbial control lab experiments, which of the following bacteria genus and species should have displayed the highest thermal death point?
A.   Neisseria sicca
B.   Escherichia coli
C.   Bacillus subtilis
D.   Staphylococcus epidermids
Question #17
Which of the following exoenzymes does the Spirit Blue Agar test for?
A.   Caseinase
B.   Gelatinase
C.   Amylase
D.   None of these
E.   Lipase
Question #18
Which of the following is a positive Starch Agar final product?
A.   glucose
B.   amino acids
C.   nucleotides
D.   fructose
E.   fatty acids
Question #19
Which of the following is the BEST reason to flame your flame loop between each Streak-for-Isolation sector?
A.   it reduces the number of bacteria as we move them from sector I to sector IV
B.   it increases the number of bacteria as we move them from sector I to sector IV
C.   it is good practice for other lab related techniques
D.   It carves a path in the agar that promotes isolation

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