Bio 2010 - Microbiology » Summer 2022 » Lab Exam 1

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Question #1
Choose the term below, that BEST completes the following statement. As magnification increases, the depth-of-field:
A.   decreases
B.   increases
C.   there is no relationship between these two
D.   stays the same
Question #2
Choose the term below, that BEST completes the following statement. As magnification increases, the resolution:
A.   stays the same
B.   decreases
C.   increases
D.   there is no relationship between these two
Question #3
Once inoculated with bacteria, how should agar Petri plates be incubated?
A.   upside down
B.   more than one of the above is correct
C.   right side up
D.   none of these
E.   sideways
Question #4
Which of the following best describes contamination?
A.   presence of carbon dioxide
B.   presence of microorganisms
C.   oxygen free
D.   presence of oxygen
E.   more than one of choices are correct
Question #5
Which of the following terms regarding the presence of microorganisms means "everywhere"?
A.   ubiquitous
B.   tropical
C.   sporadic
D.   claryvoyant
Question #6
Which of the following is the correct temperature for incubating bacteria taken from a human small intestine?
A.   10oC
B.   37oC
C.   25oC
D.   20oC
Question #7
Which of the following is the Gram's primary stain?
A.   Safranin
B.   Acetone- Alcohol
C.   Loeffler's methylene blue
D.   Crystal violet
E.   Carbolfuchsin
Question #8
Which of the following is the Gram's trapping agent?
A.   Loeffler's methylene blue
B.   Crystal violet
C.   Safranin
D.   Iodine
E.   Acetone- Alcohol
Question #9
Which of the following is the most probable genus and species of bacteria indicated by the letter “A”?
A.   Mycobacterium smegmatis
B.   Staphylococcus epidermidis
C.   Neisseria sicca
D.   Bacillus subtilis
E.   Escherichia coli
Question #10
Which compound do Ziehl-Neelsen acid-fast positive stained cells have in their cell walls?
A.   mycolic acid
B.   peptydoglycan
C.   crystal violet
D.   lactic acid
Question #11
Which of the following is the substrate found in Skim Milk Agar?
A.   triglyceride
B.   starch
C.   gelatin
D.   casein
Question #12
Which of the following is the Ziehl-Neelsen acid fast primary stain?
A.   none of these
B.   safranin
C.   carbolfuchsin
D.   acid alcohol
E.   crystal violet
Question #13
From the above image, what is the name of the green cells?
A.   endospores
B.   spores
C.   vegetative cells
D.   cysts
Question #14
Which of the following is the Shaeffer-Fulton endospore bleach or destain?
A.   none of these
B.   crystal violet
C.   safranin
D.   carbolfuchsin
E.   acid alcohol
Question #15
Which of the bacteria genus and species below produce pigmented colonies when plated on agar?
A.   Serratia marcescens
B.   Staphylococcus aureus
C.   Escherichia coli
D.   Bacillus subtilis
E.   none of these
Question #16
From our microbial control lab experiments, which of the following bacteria genus and species should have displayed the highest thermal death point?
A.   Neisseria sicca
B.   Staphylococcus epidermids
C.   Escherichia coli
D.   Bacillus subtilis
Question #17
Which of the following exoenzymes does the Spirit Blue Agar test for?
A.   Gelatinase
B.   Amylase
C.   None of these
D.   Caseinase
E.   Lipase
Question #18
Which of the following is a positive Starch Agar final product?
A.   glucose
B.   fatty acids
C.   fructose
D.   amino acids
E.   nucleotides
Question #19
Which of the following is the BEST reason to flame your flame loop between each Streak-for-Isolation sector?
A.   it reduces the number of bacteria as we move them from sector I to sector IV
B.   it is good practice for other lab related techniques
C.   It carves a path in the agar that promotes isolation
D.   it increases the number of bacteria as we move them from sector I to sector IV

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