California State University, Northridge » Biology » Biology 476 - Topics in Stem Cell Research » Fall 2022 » Pluripotent stem cell characterization methods

Question #1

Characterization is

A. an initial process done at the beginning to check authenticity of the cell line

B. done at several stages during the development of a biological product

Question #2

If cells are positive for both CD44 and TRA-1-60,

A. They are unsuitable for iPSCs

B. They would be suitable for iPSCs

Question #3

Expression of alpha feto protein indicates

A. ectoderm

B. endoderm

C. mesoderm

Question #4

Flowcytometry is used for

A. qualitative analysis of surface marker expression

B. quantitative analysis of surface marker expression

Question #5

The hPSC Score card

A. not as accurate as in vivo teratoma assays and quantifies the differentiation potential of human pluripotent stem cells

B. not as accurate as in vivo teratoma assays and is based on qPCR

C. is based on qPCR and quantifies the differentiation potential of human pluripotent stem cells

Question #6

The gold standard method for detecting aneuploidy is

A. In vivo teratoma assay

B. G-banded karyotyping

C. Comet assay