Bio 2010 - Microbiology » Summer 2023 » Lab Exam 1
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Question #1
Which of the following choices is the BEST definition of resolution?
A.
the ability to focus above or below a stained specimen
B.
the ability to distinguish two small objects as separate
C.
the distance between the end of the microscope objective lens and the specimen slide
D.
the image that one sees when looking through the oculars
Question #2
You and your partner were talking during your aseptic transfer activity. You accidentally touch your hand with the flame loop while waiting for it to cool down. Fortunately, it the loop was cool to the touch and you did not burn yourself. What should you do in order to avoid contaminating the sterile media tube in your right hand?
A.
nothing, bacteria cannot grow on the metal loop after it cools down
B.
nothing, I showered that morning, my hand should be sterile
C.
get a different flame loop
D.
start over by re-flaming the loop
Question #3
Which of the following best describes sterile media?
A.
none of these are correct
B.
media free from living organisms
C.
media free from nutrients
D.
media with low pH
Question #4
Which of the following terms regarding the presence of microorganisms means "everywhere"?
A.
sporadic
B.
ubiquitous
C.
tropical
D.
claryvoyant
Question #5
Which of the following is the correct temperature for incubating bacteria taken from a human small intestine?
A.
37oC
B.
20oC
C.
10oC
D.
25oC
Question #6
Which of the following is the Gram's secondary stain?
A.
Carbolfuchsin
B.
Loeffler's methylene blue
C.
Safranin
D.
Crystal violet
E.
Acetone- Alcohol
Question #7
Which of the following is the Gram's primary stain?
A.
Loeffler's methylene blue
B.
Crystal violet
C.
Safranin
D.
Acetone- Alcohol
E.
Carbolfuchsin
Question #8
Which of the following is the best definition of contamination?
A.
site of infection
B.
the presence of pathogens
C.
the presence of bacteria
D.
an accidental spill
Question #9
Which compound do Ziehl-Neelsen acid-fast positive stained cells have in their cell walls?
A.
crystal violet
B.
peptydoglycan
C.
lactic acid
D.
mycolic acid
Question #10
From our microbial control lab, which of the following conditions would you expect the highest percentage of bacterial cell death? Cells exposed to UV radiation in:
A.
Petri plates with plastic lids.
B.
Petri plates covered with paper.
C.
Petri plates with glass lids.
D.
Open Petri plates.
E.
Petri plates covered with aluminum foil.
Question #11
From our exoenzyme lab results, which of the following bacteria genus and species was lipase positive?
A.
Mycobacterium smegmatis
B.
Neisseria sicca
C.
Staphylococcus aureus
D.
Escherichia coli
E.
Pseudomonas fluorescens
Question #12
Which of the following is the substrate found in Skim Milk Agar?
A.
gelatin
B.
triglyceride
C.
casein
D.
starch
Question #13
Which of the following is the Ziehl-Neelsen acid fast trapping agent or mordant?
A.
crystal violet
B.
none of these are correct
C.
acid alcohol
D.
malachite green
E.
safranin
Question #14
Which of the following is the Ziehl-Neelsen acid fast bleach or destain stain?
A.
safranin
B.
crystal violet
C.
malachite green
D.
acid alcohol
Question #15
Which of the following is the Shaeffer-Fulton endospore secondary stain?
A.
safranin
B.
crystal violet
C.
acid alcohol
D.
carbolfuchsin
Question #16
Using the provided graph, provide the term for the bacteria population that appeared in the basin water at the peak labeled "A"?
A.
residents
B.
spores
C.
virulents
D.
transients
Question #17
Which of the bacteria genus and species below produce pigmented colonies when plated on agar?
A.
Staphylococcus aureus
B.
Serratia marcescens
C.
Bacillus subtilis
D.
Escherichia coli
Question #18
From our microbial control lab experiments, which of the following bacteria genus and species should have displayed the highest thermal death point?
A.
Neisseria sicca
B.
Escherichia coli
C.
Bacillus subtilis
D.
Staphylococcus epidermids
Question #19
Which of the following is a positive Starch Agar final product?
A.
fructose
B.
nucleotides
C.
amino acids
D.
fatty acids
E.
glucose
Question #20
All of the following bacteria genus and species were gelatinase positive EXCEPT?
A.
Serratia marcescens
B.
Escherichia coli
C.
Bacillus subtilis
D.
Pseudomonas fluorescens
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