Bio 2010 - Microbiology » Summer 2023 » Lab Exam 1
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Question #1
Which of the following choices is the BEST definition of resolution?
A.
the image that one sees when looking through the oculars
B.
the ability to distinguish two small objects as separate
C.
the distance between the end of the microscope objective lens and the specimen slide
D.
the ability to focus above or below a stained specimen
Question #2
You and your partner were talking during your aseptic transfer activity. You accidentally touch your hand with the flame loop while waiting for it to cool down. Fortunately, it the loop was cool to the touch and you did not burn yourself. What should you do in order to avoid contaminating the sterile media tube in your right hand?
A.
start over by re-flaming the loop
B.
get a different flame loop
C.
nothing, bacteria cannot grow on the metal loop after it cools down
D.
nothing, I showered that morning, my hand should be sterile
Question #3
Which of the following best describes sterile media?
A.
media free from nutrients
B.
none of these are correct
C.
media with low pH
D.
media free from living organisms
Question #4
Which of the following terms regarding the presence of microorganisms means "everywhere"?
A.
ubiquitous
B.
tropical
C.
sporadic
D.
claryvoyant
Question #5
Which of the following is the correct temperature for incubating bacteria taken from a human small intestine?
A.
25oC
B.
10oC
C.
37oC
D.
20oC
Question #6
Which of the following is the Gram's secondary stain?
A.
Loeffler's methylene blue
B.
Crystal violet
C.
Safranin
D.
Carbolfuchsin
E.
Acetone- Alcohol
Question #7
Which of the following is the Gram's primary stain?
A.
Loeffler's methylene blue
B.
Carbolfuchsin
C.
Crystal violet
D.
Acetone- Alcohol
E.
Safranin
Question #8
Which of the following is the best definition of contamination?
A.
an accidental spill
B.
the presence of bacteria
C.
site of infection
D.
the presence of pathogens
Question #9
Which compound do Ziehl-Neelsen acid-fast positive stained cells have in their cell walls?
A.
crystal violet
B.
peptydoglycan
C.
mycolic acid
D.
lactic acid
Question #10
From our microbial control lab, which of the following conditions would you expect the highest percentage of bacterial cell death? Cells exposed to UV radiation in:
A.
Petri plates with glass lids.
B.
Petri plates covered with aluminum foil.
C.
Petri plates covered with paper.
D.
Open Petri plates.
E.
Petri plates with plastic lids.
Question #11
From our exoenzyme lab results, which of the following bacteria genus and species was lipase positive?
A.
Pseudomonas fluorescens
B.
Neisseria sicca
C.
Staphylococcus aureus
D.
Escherichia coli
E.
Mycobacterium smegmatis
Question #12
Which of the following is the substrate found in Skim Milk Agar?
A.
casein
B.
starch
C.
triglyceride
D.
gelatin
Question #13
Which of the following is the Ziehl-Neelsen acid fast trapping agent or mordant?
A.
malachite green
B.
safranin
C.
crystal violet
D.
acid alcohol
E.
none of these are correct
Question #14
Which of the following is the Ziehl-Neelsen acid fast bleach or destain stain?
A.
crystal violet
B.
safranin
C.
acid alcohol
D.
malachite green
Question #15
Which of the following is the Shaeffer-Fulton endospore secondary stain?
A.
carbolfuchsin
B.
crystal violet
C.
safranin
D.
acid alcohol
Question #16
Using the provided graph, provide the term for the bacteria population that appeared in the basin water at the peak labeled "A"?
A.
transients
B.
residents
C.
virulents
D.
spores
Question #17
Which of the bacteria genus and species below produce pigmented colonies when plated on agar?
A.
Escherichia coli
B.
Serratia marcescens
C.
Bacillus subtilis
D.
Staphylococcus aureus
Question #18
From our microbial control lab experiments, which of the following bacteria genus and species should have displayed the highest thermal death point?
A.
Bacillus subtilis
B.
Staphylococcus epidermids
C.
Escherichia coli
D.
Neisseria sicca
Question #19
Which of the following is a positive Starch Agar final product?
A.
fructose
B.
nucleotides
C.
glucose
D.
amino acids
E.
fatty acids
Question #20
All of the following bacteria genus and species were gelatinase positive EXCEPT?
A.
Bacillus subtilis
B.
Escherichia coli
C.
Serratia marcescens
D.
Pseudomonas fluorescens
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