Bio 2010 - Microbiology » Summer 2023 » Lab Exam 1
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Question #1
Which of the following choices is the BEST definition of resolution?
A.
the ability to distinguish two small objects as separate
B.
the distance between the end of the microscope objective lens and the specimen slide
C.
the ability to focus above or below a stained specimen
D.
the image that one sees when looking through the oculars
Question #2
You and your partner were talking during your aseptic transfer activity. You accidentally touch your hand with the flame loop while waiting for it to cool down. Fortunately, it the loop was cool to the touch and you did not burn yourself. What should you do in order to avoid contaminating the sterile media tube in your right hand?
A.
nothing, bacteria cannot grow on the metal loop after it cools down
B.
nothing, I showered that morning, my hand should be sterile
C.
get a different flame loop
D.
start over by re-flaming the loop
Question #3
Which of the following best describes sterile media?
A.
media free from living organisms
B.
media with low pH
C.
media free from nutrients
D.
none of these are correct
Question #4
Which of the following terms regarding the presence of microorganisms means "everywhere"?
A.
claryvoyant
B.
tropical
C.
ubiquitous
D.
sporadic
Question #5
Which of the following is the correct temperature for incubating bacteria taken from a human small intestine?
A.
25oC
B.
20oC
C.
37oC
D.
10oC
Question #6
Which of the following is the Gram's secondary stain?
A.
Acetone- Alcohol
B.
Loeffler's methylene blue
C.
Crystal violet
D.
Safranin
E.
Carbolfuchsin
Question #7
Which of the following is the Gram's primary stain?
A.
Crystal violet
B.
Safranin
C.
Loeffler's methylene blue
D.
Carbolfuchsin
E.
Acetone- Alcohol
Question #8
Which of the following is the best definition of contamination?
A.
the presence of bacteria
B.
the presence of pathogens
C.
site of infection
D.
an accidental spill
Question #9
Which compound do Ziehl-Neelsen acid-fast positive stained cells have in their cell walls?
A.
mycolic acid
B.
peptydoglycan
C.
lactic acid
D.
crystal violet
Question #10
From our microbial control lab, which of the following conditions would you expect the highest percentage of bacterial cell death? Cells exposed to UV radiation in:
A.
Open Petri plates.
B.
Petri plates with glass lids.
C.
Petri plates covered with paper.
D.
Petri plates covered with aluminum foil.
E.
Petri plates with plastic lids.
Question #11
From our exoenzyme lab results, which of the following bacteria genus and species was lipase positive?
A.
Neisseria sicca
B.
Staphylococcus aureus
C.
Mycobacterium smegmatis
D.
Escherichia coli
E.
Pseudomonas fluorescens
Question #12
Which of the following is the substrate found in Skim Milk Agar?
A.
gelatin
B.
casein
C.
triglyceride
D.
starch
Question #13
Which of the following is the Ziehl-Neelsen acid fast trapping agent or mordant?
A.
safranin
B.
none of these are correct
C.
malachite green
D.
acid alcohol
E.
crystal violet
Question #14
Which of the following is the Ziehl-Neelsen acid fast bleach or destain stain?
A.
crystal violet
B.
malachite green
C.
acid alcohol
D.
safranin
Question #15
Which of the following is the Shaeffer-Fulton endospore secondary stain?
A.
safranin
B.
acid alcohol
C.
carbolfuchsin
D.
crystal violet
Question #16
Using the provided graph, provide the term for the bacteria population that appeared in the basin water at the peak labeled "A"?
A.
residents
B.
transients
C.
virulents
D.
spores
Question #17
Which of the bacteria genus and species below produce pigmented colonies when plated on agar?
A.
Serratia marcescens
B.
Bacillus subtilis
C.
Staphylococcus aureus
D.
Escherichia coli
Question #18
From our microbial control lab experiments, which of the following bacteria genus and species should have displayed the highest thermal death point?
A.
Escherichia coli
B.
Bacillus subtilis
C.
Neisseria sicca
D.
Staphylococcus epidermids
Question #19
Which of the following is a positive Starch Agar final product?
A.
fatty acids
B.
amino acids
C.
glucose
D.
nucleotides
E.
fructose
Question #20
All of the following bacteria genus and species were gelatinase positive EXCEPT?
A.
Pseudomonas fluorescens
B.
Serratia marcescens
C.
Bacillus subtilis
D.
Escherichia coli
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