Bio 2010 - Microbiology » Summer 2023 » Lab Quiz 2

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Question #1
Acid-fast cells resist the acid destain due to the presence of __________ in their cell envelope.
A.   phospholipids
B.   mycolic acids
C.   lipopolysaccharides
D.   peptidoglycan
Question #2
__________ is the primary stain used in the endospore stain.
A.   carbolfushin
B.   malachite green
C.   crystal violet
D.   safranin
Question #3
Following the endospore stain, vegetative (living cells) stain
A.   green
B.   pink or red
C.   purple
D.   blue
Question #4
Place the steps of the Gram stain in the correct order: 1. 95% acetone alcohol 2. crystal violet 3. safranin 4. iodine
A.   1-3-2-4
B.   1-2-3-4
C.   2-4-1-3
D.   2-1-4-3
E.   4-3-2-1
Question #5
The primary stain used in the Gram stain is
A.   safranin
B.   iodine
C.   acetone-alcohol
D.   crystal violet
Question #6
The secondary stain used in the acid-fast stain
A.   methylene blue
B.   carbolfushin
C.   safranin
D.   crystal violet
Question #7
Which of the following does not use a destain (decolorizing) agent?
A.   Gram stain
B.   endospore stain
C.   acid-fast stain
Question #8
Which of these bacterial genera are endospore-formers? 1. Clostridium 2. Staphylococcus 3. Mycobacterium 4. Bacillus
A.   1 and 4
B.   2 and 3
C.   1 and 2
D.   3 and 4

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