Bio 2010 - Microbiology » Summer 2023 » Streak-For-Isolation Technique

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Question #1
After each streak on the plate with the inoculating loop, what is done to the loop?
A.   discarded
B.   wiped with disinfectant
C.   flamed until loop glows
D.   re-introduced to stock culture
Question #2
What does the streak technique enable us to achieve?
A.   visualization of virus plaques
B.   isolation of bacterial colonies
C.   development of antibacterial microbes
D.   direct spread and dilution of bacteria from a single colony
Question #3
Where should you label your Petri plate with information about the culture?
A.   on the inside of the lid.
B.   on the top of the lid.
C.   on the bottom of the plate.
D.   on the side of the bottom (agar-containing) portion of the plate.
Question #4
A student in our class makes their first Streak-for-Isolation plate and has it returned to them after incubation. Looking at it, you notice that there is bacterial growth in the first two quadrants/sectors, but not in the last two quadrants/sectors. Which of the following is the most likely explanation for this observation?
A.   they did not incubate the agar plate at the ideal temperature
B.   they forgot to flame their loop before making the next sector/quadrant
C.   they did not incubate the agar plate long enough
D.   they did not make contact in the second sector/quadrant while making the third sector/quadrant.
Question #5
After making and incubating your Streak-for-Isolation plate a student notices that they have confluent growth in EVERY sector/quadrant. Which of the following most likely explains what happened?
A.   the tryptic soy agar plate was contaminated prior to making the streak
B.   they left the top of the Petri open too long during making the streak
C.   the bacteria spread through each sector during incubation
D.   they returned the flame loop to the broth between every sector/quadrant

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