Bio 2010 - Microbiology » Summer 2023 » Streak-For-Isolation Technique

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Question #1
After each streak on the plate with the inoculating loop, what is done to the loop?
A.   re-introduced to stock culture
B.   wiped with disinfectant
C.   discarded
D.   flamed until loop glows
Question #2
What does the streak technique enable us to achieve?
A.   direct spread and dilution of bacteria from a single colony
B.   isolation of bacterial colonies
C.   development of antibacterial microbes
D.   visualization of virus plaques
Question #3
Where should you label your Petri plate with information about the culture?
A.   on the side of the bottom (agar-containing) portion of the plate.
B.   on the inside of the lid.
C.   on the bottom of the plate.
D.   on the top of the lid.
Question #4
A student in our class makes their first Streak-for-Isolation plate and has it returned to them after incubation. Looking at it, you notice that there is bacterial growth in the first two quadrants/sectors, but not in the last two quadrants/sectors. Which of the following is the most likely explanation for this observation?
A.   they did not incubate the agar plate long enough
B.   they did not incubate the agar plate at the ideal temperature
C.   they did not make contact in the second sector/quadrant while making the third sector/quadrant.
D.   they forgot to flame their loop before making the next sector/quadrant
Question #5
After making and incubating your Streak-for-Isolation plate a student notices that they have confluent growth in EVERY sector/quadrant. Which of the following most likely explains what happened?
A.   the tryptic soy agar plate was contaminated prior to making the streak
B.   they left the top of the Petri open too long during making the streak
C.   they returned the flame loop to the broth between every sector/quadrant
D.   the bacteria spread through each sector during incubation

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