Bio 2010 - Microbiology » Spring 2023 » Gram Stain Technique Quiz

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Question #1
You forgot to use the acetone-alcohol in your Gram staining. Which of the following would be a possible consequence?
A.   Gram positive will turn purple
B.   Both Gram positive and Gram Negative will appear pink
C.   Gram negative will not be stained
D.   Both gram positive and Gram negative will appear purple
Question #2
You and your group partner were engaged in a conversation while performing your Gram stain. You realized you might have applied acetone alcohol on your smear for 1 minute. What would you do?
A.   Add crystal violet before adding safranin, which will fix the problem
B.   You did the right thing, continue with the next step
C.   Mix safranin with crystal violet
D.   Start all over again because your Gram-positive cells may have lost its primary stain
Question #3
Which of the following is the Gram's destain or bleaching reagent?
A.   Crystal violet
B.   Loeffler's methylene blue
C.   Safranin
D.   Carbolfuchsin
E.   Acetone- Alcohol

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