Bio 2010 - Microbiology » Spring 2023 » Streak-For-Isolation Technique

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Question #1
Imagine that you forgot to flame the loop before streaking the inoculum from the first sector/quadrant into the second sector/quadrant. What is the most likely consequence of this error?
A.   too much bacterial growth outside the first sector/quadrant.
B.   contamination of the broth culture.
C.   too little bacterial growth outside the first sector/quadrant.
D.   contamination of the Petri plate culture.
Question #2
Which of the following observations would suggest that a plate was inoculated with a pure culture?
A.   bacterial growth is apparent along the streaks connecting each quadrant.
B.   isolated colonies are all white in color, but some colonies are noticeably larger than others.
C.   isolated colonies have uniform color and same size.
D.   bacterial growth is apparent in all four quadrants.
Question #3
Which of the following sectors/quadrants of our Streak-for-Isolation plate should have CONFLUENT bacterial growth?
A.   II
B.   I
C.   III
D.   0
Question #4
Which of the following sectors/quadrants of our Streak-for-Isolation plate should have ISOLATED COLONIES growing in it?
A.   I
B.   III
C.   II
D.   0
Question #5
After each streak on the plate with the inoculating loop, what is done to the loop?
A.   discarded
B.   flamed until loop glows
C.   wiped with disinfectant
D.   re-introduced to stock culture

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