Bio 2010 - Microbiology » Spring 2023 » Streak-For-Isolation Technique

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Question #1
Imagine that you forgot to flame the loop before streaking the inoculum from the first sector/quadrant into the second sector/quadrant. What is the most likely consequence of this error?
A.   contamination of the Petri plate culture.
B.   contamination of the broth culture.
C.   too little bacterial growth outside the first sector/quadrant.
D.   too much bacterial growth outside the first sector/quadrant.
Question #2
Which of the following observations would suggest that a plate was inoculated with a pure culture?
A.   bacterial growth is apparent along the streaks connecting each quadrant.
B.   bacterial growth is apparent in all four quadrants.
C.   isolated colonies have uniform color and same size.
D.   isolated colonies are all white in color, but some colonies are noticeably larger than others.
Question #3
Which of the following sectors/quadrants of our Streak-for-Isolation plate should have CONFLUENT bacterial growth?
A.   I
B.   III
C.   0
D.   II
Question #4
Which of the following sectors/quadrants of our Streak-for-Isolation plate should have ISOLATED COLONIES growing in it?
A.   III
B.   0
C.   II
D.   I
Question #5
After each streak on the plate with the inoculating loop, what is done to the loop?
A.   re-introduced to stock culture
B.   flamed until loop glows
C.   discarded
D.   wiped with disinfectant

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