Bio 2010 - Microbiology » Spring 2023 » Lab Exam 1
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Question #1
What is the purpose of using oil on the oil immersion lens?
A.
to lubricate the sample slide and the objective
B.
to clean the microscope stage elevator
C.
to prevent light refraction away from the objective lens
D.
to increase contrast of the specimen
Question #2
Which of the following choices is the BEST definition of depth-of-field?
A.
the ability to focus above or below a stained specimen
B.
the image that one sees when looking through the oculars
C.
the ability to distinguish two small objects as separate
D.
the distance between the end of the microscope objective lens and the specimen slide
Question #3
How should you sterilize materials that will make contact with your culture media?
A.
wash them in the sink
B.
by using the Bunsen burner and flaming them
C.
wiping it down with a tissue
D.
you don't need to sterilize materials that will make contact with your experiment
E.
more than one of these are correct
F.
none of these
Question #4
What is the name of the instrument that we MUST use during the aseptic microorganism transfer during our lab activities?
A.
finger
B.
pipettor
C.
flame wand
D.
flame loop
Question #5
Suppose you obtained your tube with melted agar, brought it back to your desk, and subsequently forgot about the tube. When you remember, you look and discover the agar in the tube has solidified. Your lab partner says, just put it in a beaker of hot water, it will melt again. You try this with no success. The agar did not melt. Which of the following best explains why the agar did not melt?
A.
more than one of the above is correct
B.
none of these
C.
the water temperature was not hot enough
D.
agar cannot be re-melted
E.
cooling the agar it destroyed it
F.
the water temperature was not cool enough
Question #6
Which of the following terms regarding the presence of microorganisms means "everywhere"?
A.
tropical
B.
sporadic
C.
ubiquitous
D.
claryvoyant
Question #7
Which of the following is the correct temperature for incubating bacteria taken from a human small intestine?
A.
10oC
B.
20oC
C.
37oC
D.
25oC
Question #8
Which of the following is the Gram's destain or bleaching reagent?
A.
Carbolfuchsin
B.
Loeffler's methylene blue
C.
Acetone- Alcohol
D.
Crystal violet
E.
Safranin
Question #9
Which of the following is the Gram's secondary stain?
A.
Acetone- Alcohol
B.
Carbolfuchsin
C.
Loeffler's methylene blue
D.
Crystal violet
E.
Safranin
Question #10
Which of the following is the best definition of contamination?
A.
the presence of bacteria
B.
site of infection
C.
an accidental spill
D.
the presence of pathogens
Question #11
Which compound do Ziehl-Neelsen acid-fast positive stained cells have in their cell walls?
A.
peptydoglycan
B.
lactic acid
C.
crystal violet
D.
mycolic acid
Question #12
From our microbial control lab, which of the following conditions would you expect the highest percentage of bacterial cell death? Cells exposed to UV radiation in:
A.
Petri plates with glass lids.
B.
Petri plates covered with aluminum foil.
C.
Petri plates covered with paper.
D.
Open Petri plates.
E.
Petri plates with plastic lids.
Question #13
From our exoenzyme lab results, which of the following bacteria genus and species was lipase positive?
A.
Pseudomonas fluorescens
B.
Mycobacterium smegmatis
C.
Staphylococcus aureus
D.
Neisseria sicca
E.
Escherichia coli
Question #14
Which of the following is the substrate found in Skim Milk Agar?
A.
gelatin
B.
starch
C.
triglyceride
D.
casein
Question #15
Which of the following is the Ziehl-Neelsen acid fast primary stain?
A.
acid alcohol
B.
carbolfuchsin
C.
crystal violet
D.
safranin
E.
none of these
Question #16
Which of the following is the Ziehl-Neelsen acid fast secondary stain?
A.
malachite green
B.
crystal violet
C.
Loefflerâ s methylene blue
D.
safranin
E.
none of these
Question #17
Suppose we conduct a hand washing experiment. From our 0.2 ml sample. From this sample we counted 220 cells. How many colony forming units (CFU’s) are there per Liter?
A.
1.1 X E3
B.
1.1 X E5
C.
1.1 X E4
D.
1.1 X E2
E.
1.1 X E6
Question #18
Which of the following is the Shaeffer-Fulton endospore bleach or destain?
A.
safranin
B.
none of these
C.
crystal violet
D.
acid alcohol
E.
carbolfuchsin
Question #19
From our microbial control lab experiments, which of the following disinfectant/antiseptic is classified as a Quaternary Ammonium Compound (Quat)?
A.
lysol
B.
alcohol
C.
zephiran chloride
D.
bleach
Question #20
From our microbial control lab experiments, which of the following bacteria genus and species should have displayed the highest thermal death point?
A.
Neisseria sicca
B.
Escherichia coli
C.
Staphylococcus epidermids
D.
Bacillus subtilis
Question #21
Which of the following exoenzymes does the Spirit Blue Agar test for?
A.
Amylase
B.
Caseinase
C.
Lipase
D.
Gelatinase
Question #22
Which of the following sectors on a plate prepared for streaking for isolation should have isolated colony growth?
A.
II
B.
I
C.
IV
D.
III
Question #23
Which of the following is a positive Starch Agar final product?
A.
fructose
B.
glucose
C.
nucleotides
D.
amino acids
Question #24
Which of the following is the BEST reason to flame your flame loop between each Streak-for-Isolation sector?
A.
It carves a path in the agar that promotes isolation
B.
it is good practice for other lab related techniques
C.
it reduces the number of bacteria as we move them from sector I to sector IV
D.
it increases the number of bacteria as we move them from sector I to sector IV
Question #25
All of the following bacteria genus and species were gelatinase positive EXCEPT?
A.
Bacillus subtilis
B.
Pseudomonas fluorescens
C.
Escherichia coli
D.
Serratia marcescens
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