Bio 2010 - Microbiology » Spring 2023 » Lab Exam 1
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Question #1
What is the purpose of using oil on the oil immersion lens?
A.
to increase contrast of the specimen
B.
to prevent light refraction away from the objective lens
C.
to clean the microscope stage elevator
D.
to lubricate the sample slide and the objective
Question #2
Which of the following choices is the BEST definition of depth-of-field?
A.
the ability to distinguish two small objects as separate
B.
the image that one sees when looking through the oculars
C.
the ability to focus above or below a stained specimen
D.
the distance between the end of the microscope objective lens and the specimen slide
Question #3
How should you sterilize materials that will make contact with your culture media?
A.
by using the Bunsen burner and flaming them
B.
more than one of these are correct
C.
none of these
D.
wiping it down with a tissue
E.
you don't need to sterilize materials that will make contact with your experiment
F.
wash them in the sink
Question #4
What is the name of the instrument that we MUST use during the aseptic microorganism transfer during our lab activities?
A.
finger
B.
flame loop
C.
flame wand
D.
pipettor
Question #5
Suppose you obtained your tube with melted agar, brought it back to your desk, and subsequently forgot about the tube. When you remember, you look and discover the agar in the tube has solidified. Your lab partner says, just put it in a beaker of hot water, it will melt again. You try this with no success. The agar did not melt. Which of the following best explains why the agar did not melt?
A.
the water temperature was not hot enough
B.
cooling the agar it destroyed it
C.
more than one of the above is correct
D.
agar cannot be re-melted
E.
none of these
F.
the water temperature was not cool enough
Question #6
Which of the following terms regarding the presence of microorganisms means "everywhere"?
A.
tropical
B.
ubiquitous
C.
sporadic
D.
claryvoyant
Question #7
Which of the following is the correct temperature for incubating bacteria taken from a human small intestine?
A.
10oC
B.
20oC
C.
25oC
D.
37oC
Question #8
Which of the following is the Gram's destain or bleaching reagent?
A.
Acetone- Alcohol
B.
Carbolfuchsin
C.
Safranin
D.
Loeffler's methylene blue
E.
Crystal violet
Question #9
Which of the following is the Gram's secondary stain?
A.
Safranin
B.
Acetone- Alcohol
C.
Carbolfuchsin
D.
Loeffler's methylene blue
E.
Crystal violet
Question #10
Which of the following is the best definition of contamination?
A.
the presence of bacteria
B.
the presence of pathogens
C.
site of infection
D.
an accidental spill
Question #11
Which compound do Ziehl-Neelsen acid-fast positive stained cells have in their cell walls?
A.
lactic acid
B.
mycolic acid
C.
peptydoglycan
D.
crystal violet
Question #12
From our microbial control lab, which of the following conditions would you expect the highest percentage of bacterial cell death? Cells exposed to UV radiation in:
A.
Petri plates with glass lids.
B.
Petri plates covered with aluminum foil.
C.
Open Petri plates.
D.
Petri plates covered with paper.
E.
Petri plates with plastic lids.
Question #13
From our exoenzyme lab results, which of the following bacteria genus and species was lipase positive?
A.
Escherichia coli
B.
Neisseria sicca
C.
Pseudomonas fluorescens
D.
Staphylococcus aureus
E.
Mycobacterium smegmatis
Question #14
Which of the following is the substrate found in Skim Milk Agar?
A.
starch
B.
triglyceride
C.
gelatin
D.
casein
Question #15
Which of the following is the Ziehl-Neelsen acid fast primary stain?
A.
acid alcohol
B.
crystal violet
C.
safranin
D.
none of these
E.
carbolfuchsin
Question #16
Which of the following is the Ziehl-Neelsen acid fast secondary stain?
A.
malachite green
B.
Loefflerâ s methylene blue
C.
safranin
D.
none of these
E.
crystal violet
Question #17
Suppose we conduct a hand washing experiment. From our 0.2 ml sample. From this sample we counted 220 cells. How many colony forming units (CFU’s) are there per Liter?
A.
1.1 X E2
B.
1.1 X E5
C.
1.1 X E4
D.
1.1 X E6
E.
1.1 X E3
Question #18
Which of the following is the Shaeffer-Fulton endospore bleach or destain?
A.
safranin
B.
carbolfuchsin
C.
none of these
D.
crystal violet
E.
acid alcohol
Question #19
From our microbial control lab experiments, which of the following disinfectant/antiseptic is classified as a Quaternary Ammonium Compound (Quat)?
A.
zephiran chloride
B.
lysol
C.
bleach
D.
alcohol
Question #20
From our microbial control lab experiments, which of the following bacteria genus and species should have displayed the highest thermal death point?
A.
Neisseria sicca
B.
Escherichia coli
C.
Bacillus subtilis
D.
Staphylococcus epidermids
Question #21
Which of the following exoenzymes does the Spirit Blue Agar test for?
A.
Gelatinase
B.
Amylase
C.
Lipase
D.
Caseinase
Question #22
A.
II
B.
I
C.
III
D.
IV
Question #23
Which of the following is a positive Starch Agar final product?
A.
fructose
B.
glucose
C.
amino acids
D.
nucleotides
Question #24
Which of the following is the BEST reason to flame your flame loop between each Streak-for-Isolation sector?
A.
it is good practice for other lab related techniques
B.
it increases the number of bacteria as we move them from sector I to sector IV
C.
it reduces the number of bacteria as we move them from sector I to sector IV
D.
It carves a path in the agar that promotes isolation
Question #25
All of the following bacteria genus and species were gelatinase positive EXCEPT?
A.
Serratia marcescens
B.
Pseudomonas fluorescens
C.
Escherichia coli
D.
Bacillus subtilis
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