Bio 2010 - Microbiology » Spring 2023 » Lab Exam 1
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Question #1
What is the purpose of using oil on the oil immersion lens?
A.
to prevent light refraction away from the objective lens
B.
to increase contrast of the specimen
C.
to clean the microscope stage elevator
D.
to lubricate the sample slide and the objective
Question #2
Which of the following choices is the BEST definition of depth-of-field?
A.
the ability to focus above or below a stained specimen
B.
the distance between the end of the microscope objective lens and the specimen slide
C.
the image that one sees when looking through the oculars
D.
the ability to distinguish two small objects as separate
Question #3
How should you sterilize materials that will make contact with your culture media?
A.
more than one of these are correct
B.
you don't need to sterilize materials that will make contact with your experiment
C.
wiping it down with a tissue
D.
by using the Bunsen burner and flaming them
E.
wash them in the sink
F.
none of these
Question #4
What is the name of the instrument that we MUST use during the aseptic microorganism transfer during our lab activities?
A.
finger
B.
pipettor
C.
flame loop
D.
flame wand
Question #5
Suppose you obtained your tube with melted agar, brought it back to your desk, and subsequently forgot about the tube. When you remember, you look and discover the agar in the tube has solidified. Your lab partner says, just put it in a beaker of hot water, it will melt again. You try this with no success. The agar did not melt. Which of the following best explains why the agar did not melt?
A.
the water temperature was not hot enough
B.
cooling the agar it destroyed it
C.
agar cannot be re-melted
D.
more than one of the above is correct
E.
none of these
F.
the water temperature was not cool enough
Question #6
Which of the following terms regarding the presence of microorganisms means "everywhere"?
A.
sporadic
B.
ubiquitous
C.
claryvoyant
D.
tropical
Question #7
Which of the following is the correct temperature for incubating bacteria taken from a human small intestine?
A.
20oC
B.
25oC
C.
37oC
D.
10oC
Question #8
Which of the following is the Gram's destain or bleaching reagent?
A.
Acetone- Alcohol
B.
Loeffler's methylene blue
C.
Crystal violet
D.
Carbolfuchsin
E.
Safranin
Question #9
Which of the following is the Gram's secondary stain?
A.
Crystal violet
B.
Carbolfuchsin
C.
Acetone- Alcohol
D.
Safranin
E.
Loeffler's methylene blue
Question #10
Which of the following is the best definition of contamination?
A.
the presence of bacteria
B.
the presence of pathogens
C.
an accidental spill
D.
site of infection
Question #11
Which compound do Ziehl-Neelsen acid-fast positive stained cells have in their cell walls?
A.
crystal violet
B.
peptydoglycan
C.
lactic acid
D.
mycolic acid
Question #12
From our microbial control lab, which of the following conditions would you expect the highest percentage of bacterial cell death? Cells exposed to UV radiation in:
A.
Petri plates with glass lids.
B.
Open Petri plates.
C.
Petri plates covered with paper.
D.
Petri plates covered with aluminum foil.
E.
Petri plates with plastic lids.
Question #13
From our exoenzyme lab results, which of the following bacteria genus and species was lipase positive?
A.
Neisseria sicca
B.
Escherichia coli
C.
Pseudomonas fluorescens
D.
Staphylococcus aureus
E.
Mycobacterium smegmatis
Question #14
Which of the following is the substrate found in Skim Milk Agar?
A.
gelatin
B.
starch
C.
casein
D.
triglyceride
Question #15
Which of the following is the Ziehl-Neelsen acid fast primary stain?
A.
none of these
B.
safranin
C.
crystal violet
D.
carbolfuchsin
E.
acid alcohol
Question #16
Which of the following is the Ziehl-Neelsen acid fast secondary stain?
A.
none of these
B.
malachite green
C.
Loefflerâ s methylene blue
D.
safranin
E.
crystal violet
Question #17
Suppose we conduct a hand washing experiment. From our 0.2 ml sample. From this sample we counted 220 cells. How many colony forming units (CFU’s) are there per Liter?
A.
1.1 X E5
B.
1.1 X E2
C.
1.1 X E6
D.
1.1 X E3
E.
1.1 X E4
Question #18
Which of the following is the Shaeffer-Fulton endospore bleach or destain?
A.
crystal violet
B.
acid alcohol
C.
safranin
D.
carbolfuchsin
E.
none of these
Question #19
From our microbial control lab experiments, which of the following disinfectant/antiseptic is classified as a Quaternary Ammonium Compound (Quat)?
A.
alcohol
B.
zephiran chloride
C.
lysol
D.
bleach
Question #20
From our microbial control lab experiments, which of the following bacteria genus and species should have displayed the highest thermal death point?
A.
Escherichia coli
B.
Neisseria sicca
C.
Staphylococcus epidermids
D.
Bacillus subtilis
Question #21
Which of the following exoenzymes does the Spirit Blue Agar test for?
A.
Lipase
B.
Amylase
C.
Gelatinase
D.
Caseinase
Question #22
Which of the following sectors on a plate prepared for streaking for isolation should have isolated colony growth?
A.
I
B.
III
C.
II
D.
IV
Question #23
Which of the following is a positive Starch Agar final product?
A.
glucose
B.
fructose
C.
amino acids
D.
nucleotides
Question #24
Which of the following is the BEST reason to flame your flame loop between each Streak-for-Isolation sector?
A.
it reduces the number of bacteria as we move them from sector I to sector IV
B.
It carves a path in the agar that promotes isolation
C.
it is good practice for other lab related techniques
D.
it increases the number of bacteria as we move them from sector I to sector IV
Question #25
All of the following bacteria genus and species were gelatinase positive EXCEPT?
A.
Bacillus subtilis
B.
Serratia marcescens
C.
Pseudomonas fluorescens
D.
Escherichia coli
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