Bio 2010 - Microbiology » Spring 2023 » Lab Exam 1
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Question #1
What is the purpose of using oil on the oil immersion lens?
A.
to lubricate the sample slide and the objective
B.
to increase contrast of the specimen
C.
to clean the microscope stage elevator
D.
to prevent light refraction away from the objective lens
Question #2
Which of the following choices is the BEST definition of depth-of-field?
A.
the image that one sees when looking through the oculars
B.
the distance between the end of the microscope objective lens and the specimen slide
C.
the ability to focus above or below a stained specimen
D.
the ability to distinguish two small objects as separate
Question #3
How should you sterilize materials that will make contact with your culture media?
A.
more than one of these are correct
B.
by using the Bunsen burner and flaming them
C.
you don't need to sterilize materials that will make contact with your experiment
D.
wash them in the sink
E.
none of these
F.
wiping it down with a tissue
Question #4
What is the name of the instrument that we MUST use during the aseptic microorganism transfer during our lab activities?
A.
flame wand
B.
flame loop
C.
pipettor
D.
finger
Question #5
Suppose you obtained your tube with melted agar, brought it back to your desk, and subsequently forgot about the tube. When you remember, you look and discover the agar in the tube has solidified. Your lab partner says, just put it in a beaker of hot water, it will melt again. You try this with no success. The agar did not melt. Which of the following best explains why the agar did not melt?
A.
cooling the agar it destroyed it
B.
the water temperature was not hot enough
C.
none of these
D.
the water temperature was not cool enough
E.
more than one of the above is correct
F.
agar cannot be re-melted
Question #6
Which of the following terms regarding the presence of microorganisms means "everywhere"?
A.
claryvoyant
B.
ubiquitous
C.
tropical
D.
sporadic
Question #7
Which of the following is the correct temperature for incubating bacteria taken from a human small intestine?
A.
37oC
B.
10oC
C.
20oC
D.
25oC
Question #8
Which of the following is the Gram's destain or bleaching reagent?
A.
Loeffler's methylene blue
B.
Carbolfuchsin
C.
Crystal violet
D.
Safranin
E.
Acetone- Alcohol
Question #9
Which of the following is the Gram's secondary stain?
A.
Carbolfuchsin
B.
Safranin
C.
Loeffler's methylene blue
D.
Crystal violet
E.
Acetone- Alcohol
Question #10
Which of the following is the best definition of contamination?
A.
an accidental spill
B.
site of infection
C.
the presence of bacteria
D.
the presence of pathogens
Question #11
Which compound do Ziehl-Neelsen acid-fast positive stained cells have in their cell walls?
A.
crystal violet
B.
peptydoglycan
C.
lactic acid
D.
mycolic acid
Question #12
From our microbial control lab, which of the following conditions would you expect the highest percentage of bacterial cell death? Cells exposed to UV radiation in:
A.
Petri plates with glass lids.
B.
Petri plates with plastic lids.
C.
Open Petri plates.
D.
Petri plates covered with aluminum foil.
E.
Petri plates covered with paper.
Question #13
From our exoenzyme lab results, which of the following bacteria genus and species was lipase positive?
A.
Mycobacterium smegmatis
B.
Staphylococcus aureus
C.
Neisseria sicca
D.
Pseudomonas fluorescens
E.
Escherichia coli
Question #14
Which of the following is the substrate found in Skim Milk Agar?
A.
casein
B.
triglyceride
C.
gelatin
D.
starch
Question #15
Which of the following is the Ziehl-Neelsen acid fast primary stain?
A.
carbolfuchsin
B.
crystal violet
C.
safranin
D.
none of these
E.
acid alcohol
Question #16
Which of the following is the Ziehl-Neelsen acid fast secondary stain?
A.
none of these
B.
malachite green
C.
crystal violet
D.
safranin
E.
Loefflerâ s methylene blue
Question #17
Suppose we conduct a hand washing experiment. From our 0.2 ml sample. From this sample we counted 220 cells. How many colony forming units (CFU’s) are there per Liter?
A.
1.1 X E6
B.
1.1 X E3
C.
1.1 X E5
D.
1.1 X E4
E.
1.1 X E2
Question #18
Which of the following is the Shaeffer-Fulton endospore bleach or destain?
A.
safranin
B.
carbolfuchsin
C.
acid alcohol
D.
crystal violet
E.
none of these
Question #19
From our microbial control lab experiments, which of the following disinfectant/antiseptic is classified as a Quaternary Ammonium Compound (Quat)?
A.
alcohol
B.
zephiran chloride
C.
bleach
D.
lysol
Question #20
From our microbial control lab experiments, which of the following bacteria genus and species should have displayed the highest thermal death point?
A.
Neisseria sicca
B.
Escherichia coli
C.
Bacillus subtilis
D.
Staphylococcus epidermids
Question #21
Which of the following exoenzymes does the Spirit Blue Agar test for?
A.
Gelatinase
B.
Amylase
C.
Lipase
D.
Caseinase
Question #22
A.
III
B.
II
C.
I
D.
IV
Question #23
Which of the following is a positive Starch Agar final product?
A.
nucleotides
B.
fructose
C.
glucose
D.
amino acids
Question #24
Which of the following is the BEST reason to flame your flame loop between each Streak-for-Isolation sector?
A.
It carves a path in the agar that promotes isolation
B.
it increases the number of bacteria as we move them from sector I to sector IV
C.
it is good practice for other lab related techniques
D.
it reduces the number of bacteria as we move them from sector I to sector IV
Question #25
All of the following bacteria genus and species were gelatinase positive EXCEPT?
A.
Pseudomonas fluorescens
B.
Bacillus subtilis
C.
Escherichia coli
D.
Serratia marcescens
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