Bio 2010 - Microbiology » Spring 2023 » Lab Quiz 2

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Question #1
Acid-fast cells resist the acid destain due to the presence of __________ in their cell envelope.
A.   phospholipids
B.   lipopolysaccharides
C.   mycolic acids
D.   peptidoglycan
Question #2
__________ is the primary stain used in the endospore stain.
A.   malachite green
B.   carbolfushin
C.   safranin
D.   crystal violet
Question #3
Following the Gram stain, G+ and G- cells stain differently based on
A.   differences in the structure of the cell envelope
B.   differences in the chemical composition of peptidoglycan
C.   differences in the composition of the plasma membrane
D.   differences in the cytoplasm between the two cell types
Question #4
Following the endospore stain, vegetative (living cells) stain
A.   pink or red
B.   blue
C.   purple
D.   green
Question #5
Place the steps of the Gram stain in the correct order: 1. 95% acetone alcohol 2. crystal violet 3. safranin 4. iodine
A.   2-4-1-3
B.   1-2-3-4
C.   2-1-4-3
D.   1-3-2-4
E.   4-3-2-1
Question #6
The primary stain used in the Gram stain is
A.   acetone-alcohol
B.   iodine
C.   safranin
D.   crystal violet
Question #7
The secondary stain used in the acid-fast stain
A.   methylene blue
B.   safranin
C.   carbolfushin
D.   crystal violet
Question #8
Which of the following does not use a destain (decolorizing) agent?
A.   acid-fast stain
B.   endospore stain
C.   Gram stain
Question #9
Which of the following types of stains utilizes a mordant to prevent the release of the primary stain?
A.   Gram stain
B.   simple stain
C.   acid-fast stain
D.   endospore stain
Question #10
Which of these bacterial genera are endospore-formers? 1. Clostridium 2. Staphylococcus 3. Mycobacterium 4. Bacillus
A.   2 and 3
B.   1 and 2
C.   1 and 4
D.   3 and 4

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