Bio 2010 - Microbiology » Spring 2023 » Lab Quiz 2

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Question #1
Acid-fast cells resist the acid destain due to the presence of __________ in their cell envelope.
A.   peptidoglycan
B.   lipopolysaccharides
C.   phospholipids
D.   mycolic acids
Question #2
__________ is the primary stain used in the endospore stain.
A.   crystal violet
B.   carbolfushin
C.   safranin
D.   malachite green
Question #3
Following the Gram stain, G+ and G- cells stain differently based on
A.   differences in the structure of the cell envelope
B.   differences in the cytoplasm between the two cell types
C.   differences in the composition of the plasma membrane
D.   differences in the chemical composition of peptidoglycan
Question #4
Following the endospore stain, vegetative (living cells) stain
A.   green
B.   pink or red
C.   purple
D.   blue
Question #5
Place the steps of the Gram stain in the correct order: 1. 95% acetone alcohol 2. crystal violet 3. safranin 4. iodine
A.   1-3-2-4
B.   2-4-1-3
C.   4-3-2-1
D.   2-1-4-3
E.   1-2-3-4
Question #6
The primary stain used in the Gram stain is
A.   safranin
B.   iodine
C.   acetone-alcohol
D.   crystal violet
Question #7
The secondary stain used in the acid-fast stain
A.   methylene blue
B.   carbolfushin
C.   crystal violet
D.   safranin
Question #8
Which of the following does not use a destain (decolorizing) agent?
A.   acid-fast stain
B.   endospore stain
C.   Gram stain
Question #9
Which of the following types of stains utilizes a mordant to prevent the release of the primary stain?
A.   acid-fast stain
B.   endospore stain
C.   simple stain
D.   Gram stain
Question #10
Which of these bacterial genera are endospore-formers? 1. Clostridium 2. Staphylococcus 3. Mycobacterium 4. Bacillus
A.   1 and 4
B.   3 and 4
C.   1 and 2
D.   2 and 3

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