Cal State LA » Biology » Biology 1100 - Principles of Biology » Fall 2019

Question #1

The _____ pieces of DNA will run fastest on an agarose gel.

A. none of these

B. bigger

C. smaller

Question #2

You will be using ethidium bromide to ____.

A. solidify the agarose

B. move DNA down the gel

C. cause mutations

D. see the DNA

Question #3

If you don't use loading dye, what is likely to happen?

A. samples won't be visible in UV light

B. samples float out of the wells

C. samples run to slowly

D. samples run too fast

Question #4

What is the purpose of the restriction digestion?

A. all of these

B. repair the backbone of amplified DNA

C. Cut the DNA at specific sites to reveal genotype

D. none of these

E. Allow the DNA to migrate in the gel

Question #5

Running a ladder/size marker is important to ______.

A. make the gel solidify

B. make the gel run

C. have known sizes for comparison

D. keep the gel running straight